Determination of p -tyrosol and salidroside in three samples of Rhodiola crenulata and one of Rhodiola kirilowii by capillary zone electrophoresis

Abstract

A capillary zone electrophoresis (CZE) method has been developed for simultaneous assay of two bioactive components (p-tyrosol and salidroside) in Rhodiola crenulata and Rhodiola kirilowii for the first time. Those analytes were successfully separated within 15 min using 50 mmol L(-1) (pH 9.62) borate containing 30% methanol as running buffer. Regression equations revealed linear relationships (correlation coefficients 0.9998-0.9999) between peak area and concentration of the two analytes. The relative standard deviations ( RSD) of the migration times and the peak areas of two constituents ranged from 0.51 to 0.57% and from 0.65 to 1.17%, respectively, intra-day, and from 4.91 to 6.93% and from 3.51 to 5.33%, respectively, inter-day. The recoveries of two constituents ranged from 96.24 to 103.15%.

Optimization of CZE for analysis of phytochemical bioactive compounds

“…It has been found that pH had significant effect on the effective mobility of p-tyrosol and salidroside. The good separation was achieved only at pH 8.93-10.13 [48]. Therefore, optimization of pH is necessary for CZE analysis of phytochemical bioactive compounds to get good resolution with short analysis time.…”

“…Generally, at equal molarities, oligoprotic buffers like citrate will give substantially higher currents than monoprotic species such as acetate. Therefore, type and concentration of the buffer for CZE should be optimized to obtain a good resolution of the interested compounds in a satisfactory time [40,42,43,48,58,63,[87][88][89][90][91][92][93][94][95][96][97][98].…”

“…For CZE analysis of phytochemical components, different sample extraction methods, including reflux [35][36][37][38], Soxhlet extraction [39][40][41][42][43][44][45], and ultrasonic extraction [46][47][48][49][50][51][52][53][54][55][56][57][58] with different solvents, were used. In addition, some conventional methods such as shaking [59][60][61], stirring [62][63][64], marinating [65,66] and leaching [67]) were also used (Supplementary Table 1).…”

“…Therefore, organic solvents including methanol and ethanol were mainly used for Soxhelt extraction, reflux extraction or ultrasonication of analytes in raw materials. Then organic solvent was removed, and the residue was dissolved with sample buffer or organic solvent to prepare the sample solution for CZE analysis [36,37,45,48,52,55,57,61,65]. Actually, methanol extract of anthraquinones was directly diluted with buffer for CZE analysis [69].…”

Novel method of detection of phenylpropanoids of Rhodiola roots species

“…Furthermore, the medicinal properties of Rhodiola genus are comparable to those of of Ginseng and Acanthopanax. The advantage of golden root (Rhodiola rosea) is the fact that no side effects are observed [2,3]. Among the all species of Rhodiola 20 has been used in the traditional medicine of Asia: R. rosea, R. alterna, R. brevipetiolata, R. crenulata, R. kirilowii, R. quadrifida, R. sachalinensis and R. sacra [4].…”