Determination of Phenolic Compounds inArtemisia capillaris

Sheu, Shuenn‐Jyi; Tan, Ya-Wei

doi:10.1002/(sici)1521-4168(19990401)22:4<222::aid-jhrc222>3.0.co;2-6

Cited by 7 publications

(2 citation statements)

References 8 publications

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“…Since nine of the ten markers substances to be analyzed are phenols, we drew on our previous experience in separating the Chinese herb formula Cimicifuga Combination [12] wherein a mobile phase consisting of acidic phosphate buffer, acetonitrile, and methanol was used. After a series of modifications of the phosphate concentration, pH value, organic solvent composition, and column type, an ideal separation condition was obtained.…”

Section: Resultsmentioning

confidence: 99%

Determination of Phenolic Compounds in Artemisia capillaris

Sheu

Tan

1999

J. High Resol. Chromatogr.

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Section: Resultsmentioning

confidence: 99%

Determination of Phenolic Compounds in Artemisia capillaris

Sheu

Tan

1999

J. High Resol. Chromatogr.

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“…Shao-yao-tang has four ingredients (Scutellariae radix, Rhubarb, Angelicae radix, and Glycyrrhizae radix) together with I-tzutang (Cimicifuga Combination) [26]. A Cosmosil 5C 18 -AR, 5 lm, 25 cm 6 4.6 mm i.d.…”

Section: Effect Of Column Usedmentioning

confidence: 99%

Liquid Chromatographic Determination of the Constituents in Shao-yao-tang and Related Chinese Herbal Preparations

Sheu¹,

1998

J. High Resol. Chromatogr.

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A high-performance liquid chromatographic method for the separation of twenty one constituents of Shao-yao-tang including six flavonoids, six alkaloids, three anthraquinones, three carboxylic acids, one monoterpene, one saponin, and one aldehyde was developed. Detection at 275 nm with a linear gradient elution system consisting of tetrabutylammonium bromide (TBA) and acetic acid solution was found to be suitable for determination of nineteen marker substances in an unpretreated Shao-yao-tang extract within 75 min. This method can also be used to analyze 11 -14 components in a number of Chinese herbal preparations such as San-huang-hsieh-hsin-tang, Chingchieh-lien-chiao-tang, San-tsung-kuei-chien-tang, Chai-hu-ching-kantang, and Ching-yin-li-ke-tang.

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Simultaneous determination of p-hydroxyacetophenone, chlorogenic acid, and caffeic acid in Herba Artemisiae Scopariae by capillary electrophoresis with electrochemical detection

Yao

Chen

2007

Anal Bioanal Chem

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Capillary electrophoresis with electrochemical detection has been employed for the determination of p-hydroxyacetophenone, chlorogenic acid, and caffeic acid in Herba Artemisiae Scopariae (the dried sprout of Artemisia scoparia Waldst. et Kit.). The effects of several important factors, such as the concentration and the acidity of the running buffer, separation voltage, injection time, and detection potential, were investigated to acquire the optimum conditions. The detection electrode was a 300-microm-diameter carbon disc electrode at a working potential of +0.90 V (relative to the saturated calomel electrode). The three analytes can be well separated within 11 min in a 40-cm-long fused-silica capillary at a separation voltage of 15 kV in 50 mM borate buffer (pH 9.2). The relation between peak current and analyte concentration was linear over about 3 orders of magnitude, with detection limits (signal-to-noise ratio of 3) of 0.31, 0.39, and 0.50 microM for p-hydroxyacetophenone, chlorogenic acid, and caffeic acid, respectively. The proposed method has been successfully applied to monitor the three bioactive constituents in real plant samples and to differentiate between different herbal drugs with satisfactory assay results.

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Determination of Phenolic Compounds inArtemisia capillaris

Cited by 7 publications

References 8 publications

Determination of Phenolic Compounds in Artemisia capillaris

Determination of Phenolic Compounds in Artemisia capillaris

Liquid Chromatographic Determination of the Constituents in Shao-yao-tang and Related Chinese Herbal Preparations

Simultaneous determination of p-hydroxyacetophenone, chlorogenic acid, and caffeic acid in Herba Artemisiae Scopariae by capillary electrophoresis with electrochemical detection

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