Determination of some estrogens by flow injection analysis with acidic potassium permanganate–formaldehyde chemiluminescence detection

Liao, Sulan; Wu, Xiaoping; Xie, Zenghong

doi:10.1016/j.aca.2005.01.005

Cited by 53 publications

(28 citation statements)

References 25 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The mechanism of enhancement by formaldehyde and related compounds has, as yet, not been fully elucidated. Various research groups have postulated that these species are oxidized to form excited-state carbon dioxide 24,25 or singlet oxygen; 26,27 some have even suggested that these enhancers increase the rate of analyte oxidation. 28 This study reports on a flow injection chemiluminescence (FI-CL) system for the determination of retinol and tocopherol, based on their enhancement effect on the KMnO4-HCHO CL reaction in an acidic medium.…”

Section: Introductionmentioning

confidence: 99%

Flow-Injection Determination of Retinol and Tocopherol in Pharmaceuticals with Acidic Potassium Permanganate Chemiluminescence

et al. 2009

View full text Add to dashboard Cite

Section: Introductionmentioning

confidence: 99%

Flow-Injection Determination of Retinol and Tocopherol in Pharmaceuticals with Acidic Potassium Permanganate Chemiluminescence

et al. 2009

View full text Add to dashboard Cite

“…25 Obviously, the established ELISA method is more sensitive than the existing chromatographic methods, the detection limits of which for DES were 0.1-0.2 ng/mL for HPLC, 3 0.1 ng/mL for GC-MS 6 and 1.08 ng/mL for CL. 9 Compared with the existing chromatographic methods, ELISA using MTPs tends to be high specific, simple, inexpensive with a large quantity of sample analyses done capably. Furthermore, potentiality to be developed into portable test kits makes ELISA a promising tool to detect DES at low concentration levels.…”

Section: Sensitivity and Specificity Of Anti-des Pabsmentioning

confidence: 99%

“…Urine and faeces of treated animals and feed of animals were also detected to achieve early detection of contravention of the regulations. The instrumental methods such as high-performance liquid chromatography (HPLC), 2,3 gas chromatography (GC), 4 GC-mass spectrometry (GC-MS), 5,6 colorimetric assay 7 and chemiluminescence (CL) 8,9 have been used to detect DES residue, but they are laborious, expensive, timeconsuming and need effort for sample preparation before analysis. These conventional methods are not suitable to screen samples routinely.…”

Section: Introductionmentioning

confidence: 99%

Development of an Indirect Competitive ELISA Based on Polyclonal Antibody for the Detection of Diethylstilbestrol in Water Samples

Wang

Ling

et al. 2007

Chin. J. Chem.

View full text Add to dashboard Cite

An indirect competitive enzyme-linked immunosorbent assay (icELISA) based on polyclonal antibody for the estrogen diethylstilbestrol (DES) was developed. With this aim, two different haptens mono-O-3-carboxypropyldiethylstilbestrol (DES-CP) and mono-O-carboxymethyldiethylstilbestrol (DES-CM) with carboxylic group that preserve the molecular structure character of diethylstilbestrol were synthesized. The haptens were conjugated with the carrier proteins bovine serum albumin (BSA) by mixed-anhydride method for immunogen and conjugated with ovalbumin (OVA) by active ester method for coating antigen. Polyclonal antibodies for diethylstilbestrol were raised by immunizing mice with immune antigen DES-CP-BSA. Under optimized system, the lowest limit of detection (LLD) of diethylstilbestrol was 0.01 ng/mL, and IC 50 ＝1.02 ng/mL. Its analogs were tested and no obvious cross-reactivity was found to anti-diethylstilbestrol antibody. DES-fortified water samples were determined by simple dilution to diminish the matrix effect. The comparison between the amount of DES estimated by ELISA and the amount added indicates good agreement for all water samples tested, with mean recovery values ranging from 86% to 120.2%.

show abstract

“…Acidic potassium permanganate is one of the most important oxidants applied in CL reactions and a great number of investigations have been published (25)(26)(27)(28)(29)(30)(31)(32).…”

Section: Introductionmentioning

confidence: 99%

Development of a sensitive flow‐injection–chemiluminescence detection method for the determination of laevodopa

et al. 2008

View full text Add to dashboard Cite

A simple chemiluminometric method using flow injection has been developed for the determination of levodopa, based on its sensitizing effect on the weak chemiluminescence (CL) reaction between Na(2)SO(3) and acidic KMnO4. Under optimum experimental conditions, the CL intensity was linearly related to the concentration of levodopa from 3.4 x 10(-8) to 2.4 x 10(-5) mol/L and the detection limit was 1.1 x 10(-8) mol/L (s:n = 3). The relative standard deviation (RSD) of the proposed method calculated from 20 replicate injection of 3 x 10(-7) mol/L levodopa was 3.3%. The correlation coefficient was 0.997. The method was successfully applied to the determination of levodopa in commercial pharmaceutical formulations and spiked urine samples.

show abstract

Determination of some estrogens by flow injection analysis with acidic potassium permanganate–formaldehyde chemiluminescence detection

Cited by 53 publications

References 25 publications

Flow-Injection Determination of Retinol and Tocopherol in Pharmaceuticals with Acidic Potassium Permanganate Chemiluminescence

Flow-Injection Determination of Retinol and Tocopherol in Pharmaceuticals with Acidic Potassium Permanganate Chemiluminescence

Development of an Indirect Competitive ELISA Based on Polyclonal Antibody for the Detection of Diethylstilbestrol in Water Samples

Development of a sensitive flow‐injection–chemiluminescence detection method for the determination of laevodopa

Contact Info

Product

Resources

About