Determination of the cumulative and skin-resorptive action of the Zoodizin disinfectant

Abstract

The rational organization and implementation of effective disinfection activities plays an important role in the complex of measures for the prevention of infections. The development and introduction of new disinfectants into production is an urgent issue of modern poultry farming. When developing a disinfectant, it is important to determine the cumulative effect of the drug. The purpose of the study was to determine the cumulative and skin-resorptive action of the disinfectant “Zoоdizin”. For the toxicological study of the drug used healthy white male rats and white female rats weighing 200 ± 10 g 1.5 years of age. To study the toxicity of the drug “Zoоdizin” when applied to the skin used the method of immersion of the tails of rats in a test tube with the test substance. The tail was injected 2/3 into a regular tube with a 5 % solution of the drug “Zoоdizin”. The tube was closed with a cork ring whose diameter was slightly larger than the tail diameter. For 15 days, the tubes were placed daily in a water bath at 28–30 °C for 2 hours. Control animals tails were immersed in distilled water. To establish the local action of the drug “Zoodizin” on the mucous membranes of the study drug was introduced into the conjunctival sac of the right eye of the rabbit at a dose of 50 mg, and in the left eye was buried saline in a volume of 0,05 cm3. When studying the cumulative effect of Zoоdizin, no significant changes in the biochemical parameters in the serum of rats were observed. In the study of possible irritant or damaging effect on the skin and the development of contact non-allergic dermatitis found that a single application of disinfectant “Zoоdizin” on the unaffected skin of the back of white rats in the maximum significant recommended concentration of working solutions (2 %) did not cause signs. The single effect of the drug on the intact areas of the skin did not cause skin irritation, but it can be stated that prolonged daily epicutaneous exposure of high concentration (5 %) of the solution of the drug “Zoоdizin”, which is 2.5 times higher than the maximum recommended concentration, caused a general resorption. When assessing the cumulative properties, it was taken into account that the total dose administered to rats was Zodizin 42000 mg/kg body weight and did not result in animal death. It did not allow to calculate the cumulative coefficients for the “lethal effect”. A single effect of the product on the intact areas of the skin did not cause skin irritation, but it can be stated that prolonged daily epicutaneous exposure of a high concentration (5 %) of the Zodizin solution, which is 2.5 times the maximum recommended concentration, caused a general resorption. In the future, it is planned to study the virulidal properties of the biocide “Zoоdizin”.

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“…Зрілі віріони круглої форми, мають подвійну оболонку, щільну серцевину та бокові тільця. За своєю морфологією вірус ідентичний з вірусом віспи (Nevolko et al, 2017;Nechyporenko et al, 2020).…”