An important element in ensuring the epizootic well-being of the poultry industry is disinfection. Modern poultry farming requires a large number of effective disinfectants. It is known that the resistance of microorganisms to the effects of disinfectants is based on a genotypic mechanism. The nature of the formation of resistance to disinfectants and antiseptics is different than antibiotics. With regard to disinfectants, resistance is formed more slowly and the proportion of resistant strains in the population of microorganisms may not be high for a long time. This is due to different mechanisms of formation of resistance to antibiotics and disinfectants, in the first case – plasmid mechanism, in the second – chromosomal. However, increasing the resistance to the active substance in disinfectants can be widespread, so it is necessary to periodically rotate disinfectants. The goal of the work – to investigate the parameters of acute toxicity of the disinfectant biocide “Zodizin”. The studies were conducted in the laboratory of Veterinary Pharmacy and the Vivarium of Sumy National Agrarian University. The drug “Zodizine” contains: polyhexamethyleneguanidine hydrochloride – 21.0 %, alkylldimethylbenzylammonium chloride – 3.0 %. For toxicological examination of the disinfectant, healthy white male rats and white female rats weighing 200 ± 10 g 1.5 years of age were used. In the study of acute toxicity of animals observed daily, noted the general condition of the animals, features of their behavior. Studies have found that the toxic effect of the disinfectant “Zodizin” clinically manifested almost equally in both males and females. The average lethal dose for the rat female was 1000.0 ± 35.0 mg/kg body weight, males 1033.0 ± 34.3 mg/kg. Therefore, according to the classification of substances by toxicity, the drug by intragastric administration can be attributed to low-toxic substances. Observations on animals revealed that 1–3 hours after oral administration of the drug in a subtoxic dose in laboratory animals, shortness of breath and inhibition of the central nervous system were noted. Most of them died during the first day. Subsequent observations of the surviving animals indicated that their motor response was suppressed over the next 24–72 hours. Conclusions and prospects for further research: 1. It was found that the average lethal dose of the drug “Zodizin” with oral administration to rats-females was 1000.0 ± 35.0 mg/kg body weight, males – 1033.0 ± 34.3 mg/kg. 2. Experimental studies have proved that the disinfectant “Zodizin” according to GOST 12.1.007-76, belongs to the IV class of danger, that is, to the low-dangerous compounds, and according to GOST 12.1.07 – to the III class of hazard of substances and can be used for disinfection premises where animals and poultry are kept. Further, the sporoсide and corrosion properties of the “Zoodizin” biocide will be studied.

The key to successful work of a modern poultry industry and the safety of its products for consumers is high-quality disinfection. Modern disinfectants used in poultry farms are different in composition, concentration of active substances, means of application and other properties. Most of them are represented by imported products. Their replacement in the domestic market with effective, safe and economical drugs is extremely relevant. It should be highly effective means, possessing a wide spectrum of virilicidal, bactericidal, sporocidal and fungicidal action. The goal of the work. The purpose of our research was to evaluate the effectiveness of the complex disinfection in the production conditions of the Bi-Dez and DezSan products. Materials and methods of research. Production testing of the disinfection of poultry facilities and equipment using the Bi-dez and DezSan schemes was carried out at one of the poultry farms of the Sumy region. At the first stage, the water supply system was treated by filling the piping line with a 0.1% solution of Bi-dez with an exposure of 1 hour. At the second stage, moisture was disinfected with 0.8% solution DezSan from the rate of 0.2-0.3 liters of solution per 1 m 2 , exposure time -2-3 hours. Then, in the room, the fine dispersed aerosol of this biocide was sprayed into a 10% solution (1 liter of preparation per 9 liters of water) -aerosol disinfection by fogging at a rate of 5 ml of solution per 1 m 3 of space, exposure time -at least 3 hours. Results of research and discussion. Evaluating the effectiveness of disinfection of the water supply system with the Bi-dez compound, found that the total microbial contamination of the water before treatment was 235 CFU/ml. In addition to other bacteria, it was isolated from the intestinal bacillus. After the rehabilitation, the microbial water pollution decreased to 4 CFU/ml. Sanitary-indicative microorganisms (E. coli, salmonella and staphylococci) were not detected in the system faults. The total amount of microorganisms in the air after disinfection with the drug DezSan decreased by 2.6 times (12 thousand mc/m 3 ) compared with the baseline bacterial background (29 thousand mc/m 3 ), and the intestinal rod after treatment from the selected in the poultry house air samples were not allocated. Conclusions and prospects for further research. Reducing the microbial background and the death of opportunistic microorganisms in the premises and the system of drinking poultry farms contribute to the veterinary well-being of poultry farms. In the future, it is planned to conduct a study of the drug DezSan, identified it irritating, skinresorptive, cumulative and other properties.

The article presents data on the study of corrosion activity and foaming properties of biocide "DezSan". One of the main characteristics of a biocide is its corrosive activity. The use of a biocide in a poultry house involves getting it on metal, rubber, plastic surfaces, and the like. The biocide should provide minimal aggressive effect on the surface data. In the industrial poultry industry, the most frequently used equipment with surfaces made of stainless steel, aluminum and galvanized iron. Also, when choosing a biocide, pay attention to its foaming properties. They should not exceed 40%, as this ensures a quality disinfection. One of the key points is the index of foam stability, which significantly affects the time of contact with the biocide and provides effective decontamination of the surfaces. The goal of the work. To determine the corrosion activity and foaming properties of the new domestic biocide "DezSan". Materials and methods of research. In their experiments, a disinfection tool for the production of the scientific and production company "Brovаfarma" (Ukraine) "DesSan" was used. Relative corrosion activity of various concentrations of biocide "DezSan" was determined in comparison with the standard -1.5% solution of sodium hydroxide. To determine the foaming properties of biocide "DezSan" used the method of Ross-Miles (1983). Results of research and discussion. The biocide "DezSan" in all experimented concentrations (0.25-1.5%) has a minor corrosion effect on aluminum, stainless and galvanized steel, compared with the standard (1.5% NaOH solution). The biocide "DezSan" does not cause deformation when exposed to metal samples. "DezSan" in various concentrations has a foaming property of 13.1 to 37.3%, which increased directly in proportion to increasing the concentration of biocides. The foaming capacity of "DezSan" did not exceed 40%, and the foam stability at these concentrations varied within 0,08-0,19. Conclusions and prospects for further research. Thus, the biocide "DezSan" has low corrosion properties in relation to aluminum, stainless steel and galvanized steel in comparison with the standard (1.5% solution of NaOH). The biocide "DezSan" does not cause deformation when exposed to metal samples. The foaming capacity was not more than 40%, and the foam resistance was up to 0.19. These indicators allow the wide use of biocide "DezSan" in the poultry industry. In the future, it is planned to conduct a study of the biocid " DezSan", identified it irritating, skin-resorptive, cumulative and other properties.

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The rational organization and implementation of effective disinfection activities plays an important role in the complex of measures for the prevention of infections. The development and introduction of new disinfectants into production is an urgent issue of modern poultry farming. When developing a disinfectant, it is important to determine the cumulative effect of the drug. The purpose of the study was to determine the cumulative and skin-resorptive action of the disinfectant “Zoоdizin”. For the toxicological study of the drug used healthy white male rats and white female rats weighing 200 ± 10 g 1.5 years of age. To study the toxicity of the drug “Zoоdizin” when applied to the skin used the method of immersion of the tails of rats in a test tube with the test substance. The tail was injected 2/3 into a regular tube with a 5 % solution of the drug “Zoоdizin”. The tube was closed with a cork ring whose diameter was slightly larger than the tail diameter. For 15 days, the tubes were placed daily in a water bath at 28–30 °C for 2 hours. Control animals tails were immersed in distilled water. To establish the local action of the drug “Zoodizin” on the mucous membranes of the study drug was introduced into the conjunctival sac of the right eye of the rabbit at a dose of 50 mg, and in the left eye was buried saline in a volume of 0,05 cm3. When studying the cumulative effect of Zoоdizin, no significant changes in the biochemical parameters in the serum of rats were observed. In the study of possible irritant or damaging effect on the skin and the development of contact non-allergic dermatitis found that a single application of disinfectant “Zoоdizin” on the unaffected skin of the back of white rats in the maximum significant recommended concentration of working solutions (2 %) did not cause signs. The single effect of the drug on the intact areas of the skin did not cause skin irritation, but it can be stated that prolonged daily epicutaneous exposure of high concentration (5 %) of the solution of the drug “Zoоdizin”, which is 2.5 times higher than the maximum recommended concentration, caused a general resorption. When assessing the cumulative properties, it was taken into account that the total dose administered to rats was Zodizin 42000 mg/kg body weight and did not result in animal death. It did not allow to calculate the cumulative coefficients for the “lethal effect”. A single effect of the product on the intact areas of the skin did not cause skin irritation, but it can be stated that prolonged daily epicutaneous exposure of a high concentration (5 %) of the Zodizin solution, which is 2.5 times the maximum recommended concentration, caused a general resorption. In the future, it is planned to study the virulidal properties of the biocide “Zoоdizin”.