Determination of Total Antioxidant Capacity in Plasma by Cyclic Voltammetry. Two Case Reports

Abstract

The cyclic voltammetry (CV) was used for the measurement of the plasma total antioxidant capacity from two types of patients. The first one consisted of 29 volunteers (men aged 18-21 years) who were administered placebo or silymarin at a dose of 858 mg/day. After two months of silymarine administration, CV revealed a statistically significant increase in total antioxidant capacity compared to placebo. No statistically significant changes in TBARS, SH-groups, creatininin, urea, and uric acid concentrations were found. The second group under study comprised 49 patients with chronic renal disease during dialysis therapy. After dialysis, CV revealed a decrease of total antioxidant capacity in the plasma, which was equivalent to a decrease in creatinine, urea and uric acid. CV was performed using a system consisting of a working glassy carbon electrode, an auxiliary platinum electrode, and a reference saturated calomel electrode; a linear change of voltage of 200 mV/s was applied. CV is a simple and relatively reliable method for assessment of body antioxidant status. It is also time and cost effective.

The effect of silymarin (Silybum marianum) on human skin fibroblasts in anin vitrowound healing model

“…Antioxidant activity of silymarin was previously demonstrated in vitro and in vivo. Most of these reported antioxidant activities were based on cell-free biochemical tests (Psotová et al, 2001;Asghar & Masood, 2008;Turgut et al, 2008;KÖksal et al, 2009).…”

Antocianinas, ácido ascórbico, polifenoles totales y actividad antioxidante, en la cáscara de camu-camu (Myrciaria dubia (H.B.K) McVaugh)

“…Este radical, es utilizado ampliamente para medir la actividad antioxidante en el plasma sanguíneo, y fluidos biológicos (RE et al, 1999;PSOTOVÁ et al, 2001), así como para los carotenoides y compuestos fenólicos (PELLEGRINI et al, 1999), adicionalmente se puede medir el potencial antioxidante de muestras de drogas (CALBIOCHEM, 2000).…”

The Role of Oxidative Stress and Inflammatory Mediators in Benign Paroxysmal Positional Vertigo

“…Vitamins C, vitamin E, albumin, bilirubin, and uric acid are the main antioxidants contributing to the TAC levels of serum. TAC gives important data about the total status of antioxidation events in the body [5] . Paraoxonase (PON) is an enzyme that hydrolyzes paraoxon, a toxic metabolite of organophosphate molecules.…”