Determination of trace metallothioneins at nanogram levels with Eosin Y by resonance light scattering method

Xiao, Xilin; Xue, Jiaying; Ding, Dexin; Liao, Lifu; Meng, Xialing; Liu, Liangzhuan; Zhou, Bin; He, Bo

doi:10.1080/03067319.2015.1048436

Cited by 4 publications

(2 citation statements)

References 39 publications

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“…Chromatographic methods for MT determination include size exclusion chromatography (Wolf et al, 2009), ion exchange (Infante et al, 2006), and two-dimensional high-performance liquid chromatography (HPLC) coupled with ICP-MS (Miyayama et al, 2007). Resonance light scattering (RLS) was applied using Eosin Y as a dye (Xiao et al, 2015) or phenantroline-Cu chelate as a probe (Xiao et al, 2014) and revealed a cost effective and rapid technique for the determination of MTs at nanomolar levels.…”

Section: Current Analytical Techniques For the Determination Of Mts And Pcsmentioning

confidence: 99%

Microalgal Metallothioneins and Phytochelatins and Their Potential Use in Bioremediation

et al. 2020

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The persistence of heavy metals (HMs) in the environment causes adverse effects to all living organisms; HMs accumulate along the food chain affecting different levels of biological organizations, from cells to tissues. HMs enter cells through transporter proteins and can bind to enzymes and nucleic acids interfering with their functioning. Strategies used by microalgae to minimize HM toxicity include the biosynthesis of metal-binding peptides that chelate metal cations inhibiting their activity. Metalbinding peptides include genetically encoded metallothioneins (MTs) and enzymatically produced phytochelatins (PCs). A number of techniques, including genetic engineering, focus on increasing the biosynthesis of MTs and PCs in microalgae. The present review reports the current knowledge on microalgal MTs and PCs and describes the state of art of their use for HM bioremediation and other putative biotechnological applications, also emphasizing on techniques aimed at increasing the cellular concentrations of MTs and PCs. In spite of the broad metabolic and chemical diversity of microalgae that are currently receiving increasing attention by biotechnological research, knowledge on MTs and PCs from these organisms is still limited to date.

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Section: Current Analytical Techniques For the Determination Of Mts And Pcsmentioning

confidence: 99%

Microalgal Metallothioneins and Phytochelatins and Their Potential Use in Bioremediation

et al. 2020

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“…The linear concentration ranges were 0.01-0.5 and 0.014-2.5 μg ml −1 . A ternary complex was formed between metallothioneins (Xiao et al 2015), eosin Y and sodium dodecyl benzene sulfate in Britton-Robinson buffer (pH 3.9). The RRS of the complex was measured at 366 nm and the method had a linear concentration range of 0.04-14.0 μg ml −1 .…”

Section: Resonance Rayleigh Scatteringmentioning

confidence: 99%

Application of a xanthene dye, eosin Y, as spectroscopic probe in chemical and pharmaceutical analysis; a review

Derayea

Nagy

2018

Reviews in Analytical Chemistry

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Abstract Eosin Y (EY) is an acidic xanthene dye which is mainly used in food stuff and biological staining. Various analytical methods have been reported for the utility of this dye in the quantitative determination of several pharmaceutical compounds, heavy metals in addition to some surfactants and proteins. Most of the applied methods were based on the formation of association complexes between eosin Y and the target analytes in buffered aqueous solutions. The present article represents a comprehensive review for the use of eosin Y as a probe in analytical chemistry.

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Interaction quantitative modeling of mixed surfactants for synergistic solubilization by resonance light scattering

Xiang

Zhen

You

et al. 2021

Environ Sci Pollut Res

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Determination of trace metallothioneins at nanogram levels with Eosin Y by resonance light scattering method

Cited by 4 publications

References 39 publications

Microalgal Metallothioneins and Phytochelatins and Their Potential Use in Bioremediation

Microalgal Metallothioneins and Phytochelatins and Their Potential Use in Bioremediation

Application of a xanthene dye, eosin Y, as spectroscopic probe in chemical and pharmaceutical analysis; a review

Interaction quantitative modeling of mixed surfactants for synergistic solubilization by resonance light scattering

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