Determination of vitamin B12 in multivitamin tablets by multimode high-performance liquid chromatography

Wongyai, Surapote

doi:10.1016/s0021-9673(99)01200-5

Cited by 48 publications

(20 citation statements)

References 7 publications

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“…30 Many of the studies developed for the quantification of vitamins in various matrices also include extraction procedures, and the solvents used differ according to the type of vitamin (fat-soluble or water-soluble). For the separate extraction of fat-and water-soluble vitamins in various matrices, solvents such as methanol:water, 31 methanol, 32 ethanol:orthophosphoric acid:hexane, 33 and ethanol:water 34,35 have been used. In addition, for the extraction of all of the fat-and water-soluble vitamins, a mixture of TFA:methanol 18 and a column technique with SPE extraction 24 have been used.…”

Section: Optimization Of the Extraction Methodsmentioning

confidence: 99%

Simultaneous and accurate determination of water- and fat-soluble vitamins in multivitamin tablets by using an RP-HPLC method

Küçükkolbaşı¹,

Bilber²,

Ayyıldız³

et al. 2013

Quím. Nova

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Recebido em 14/10/12; aceito em 22/2/13; publicado na web em 24/5/13In the present study, a reversed-phase high-performance liquid chromatographic (RP-HPLC) procedure was developed and validated for the simultaneous determination of seven water-soluble vitamins (thiamine, riboflavin, niacin, cyanocobalamin, ascorbic acid, folic acid, and p-aminobenzoic acid) and four fat-soluble vitamins (retinol acetate, cholecalciferol, α-tocopherol, and phytonadione) in multivitamin tablets. The linearity of the method was excellent (R 2 > 0.999) over the concentration range of 10-500 ng mL −1. The statistical evaluation of the method was carried out by performing the intra-and inter-day precision. The accuracy of the method was tested by measuring the average recovery; values ranged between 87.4% and 98.5% and were acceptable quantitative results that corresponded with the label claims.

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Section: Optimization Of the Extraction Methodsmentioning

confidence: 99%

Simultaneous and accurate determination of water- and fat-soluble vitamins in multivitamin tablets by using an RP-HPLC method

Küçükkolbaşı¹,

Bilber²,

Ayyıldız³

et al. 2013

Quím. Nova

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“…The mobile phase of the HPLC system delivered at a flow rate of 0. 8 Detection was performed with a photodiode array detector monitoring the eluent at 280 nm; however, quantitation was performed at maximum wavelength for each vitamin as follows: 270 nm for thiamine, 265 nm for riboflavin, 260 nm for nicotinic acid, 256 nm for nicotinamide, 257 nm for pyridoxine, 230 nm for cyanocobalamin, and 280 nm for folic acid. Identification of resolved peaks in real samples has been executed by comparing their spectra with those derived from standard solutions.…”

Section: Chromatographic Conditionsmentioning

confidence: 99%

Development of a validated HPLC method for the determination of B‐complex vitamins in pharmaceuticals and biological fluids after solid phase extraction

Chatzimichalakis

Samanidou

Verpoorte

et al. 2004

J of Separation Science

109

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An HPLC method was developed for the simultaneous determination of seven water-soluble vitamins, viz. thiamine, riboflavin, nicotinic acid, nicotinamide, pyridoxine, cyanocobalamin, and folic acid, in multivitamin pharmaceutical formulations and biological fluids (blood serum and urine). Separation was achieved at ambient temperature on a Phenomenex Luna C18 (150 x 4.6 mm) analytical column. Gradient elution was performed starting at a 99:1 A:B v/v composition, where A: 0.05 M CH3COONH4/CH3OH (99/1) and B: H2O/CH3OH (50/50), at a flow rate of 0.8 mL/min. After a 4-min isocratic elution the composition was changed to 100% of B in 18 min and elution continued isocratically for 8 min. Detection was performed with a photodiode array detector at 280 nm. Each vitamin was quantitatively determined at its maximum wavelength. Spectral comparison was used for peak identification in real samples. Detection limits were in the range of 1.6-3.4 ng, per 20-microL injection, while linearity held up to 25 ng/microL. Accuracy, intra-day repeatability (n = 6), and inter-day precision (n = 7) were found to be satisfactory. Theobromine (2 ng/microL) was used as internal standard. Sample preparation of biological fluids was performed by SPE on Supelclean LC-18 cartridges with methanol-water 85/15 v/v as eluent. Extraction recoveries from biological matrices ranged from 84.6% to 103.0%.

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“…New vitamin B 1 -selective electrodes with polyvinylchloride membrane were developed that contain ion associates of vitamin B 1 with inorganic and organic counter-ions BiI 4 À and brilliant yellow as electrode-active substances. The proposed electrodes exhibit long lifetime, good stability, sensitivity, precision, accuracy and selectivity.…”

Section: Discussionmentioning

confidence: 99%

“…A significant share is contributed by optical [24,25] and other types of sensors [26,27]. In this paper we present new forms of ion associates (IAs) of vitamin B 1 with an inorganic anion BiI 4 À and an organic anion brilliant yellow BY 2À ( Figure 1) that were successfully used as electrode-active substances (EASs) for the preparation of PVC-based potentiometric sensors for the determination of vitamin B 1 .…”

Section: Introductionmentioning

confidence: 99%

Design of a Vitamin B₁‐Selective Electrode Based on an Ion‐Pair and Its Application to Pharmaceutical Analysis

et al. 2010

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Vitamin B 1 -selective electrodes with PVC membrane were developed that contain ion associates of vitamin B 1 with an inorganic anion, BiI 4 À , and an organic anion, brilliant yellow, as electrode-active substances. The linearity ranges of the electrode function are 1.0 10 À5 -1.0 10 À2 and 1.0 10 À4 -1.0 10 À2 M, the electrode function slopes are 33.0 AE 1.0 and 33.1 AE 1.1 mV decade À1 , the detection limits are 5.5 10 À6 and 8.3 10 À5 M for BiI 4 À and brilliant yellow respectively. The working range of pH is 5-12. The efficiency of the use of electrodes for the vitamin B 1 content control in multivitamin pharmaceutical preparations was shown by direct potentiometry and potentiometric titration methods.

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Determination of vitamin B12 in multivitamin tablets by multimode high-performance liquid chromatography

Cited by 48 publications

References 7 publications

Simultaneous and accurate determination of water- and fat-soluble vitamins in multivitamin tablets by using an RP-HPLC method

Simultaneous and accurate determination of water- and fat-soluble vitamins in multivitamin tablets by using an RP-HPLC method

Development of a validated HPLC method for the determination of B‐complex vitamins in pharmaceuticals and biological fluids after solid phase extraction

Design of a Vitamin B₁‐Selective Electrode Based on an Ion‐Pair and Its Application to Pharmaceutical Analysis

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Determination of vitamin B12 in multivitamin tablets by multimode high-performance liquid chromatography

Cited by 48 publications

References 7 publications

Simultaneous and accurate determination of water- and fat-soluble vitamins in multivitamin tablets by using an RP-HPLC method

Simultaneous and accurate determination of water- and fat-soluble vitamins in multivitamin tablets by using an RP-HPLC method

Development of a validated HPLC method for the determination of B‐complex vitamins in pharmaceuticals and biological fluids after solid phase extraction

Design of a Vitamin B1‐Selective Electrode Based on an Ion‐Pair and Its Application to Pharmaceutical Analysis

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Design of a Vitamin B₁‐Selective Electrode Based on an Ion‐Pair and Its Application to Pharmaceutical Analysis