Development and validation of kinetic and atomic absorption spectrophotometric methods for the determination of salbutamol sulfate

Abstract

Two sensitive kinetic and atomic absorption spectrophotometric (AAS) methodswhere developed for the determination of salbutamol sulfate (SLS) in its dosage forms. The kinetic method was based on the bromination reaction of the drug by bromine generated in-situ by the reaction of bromate with bromide in acidic medium.The reaction was followed spectrophotometrically by measuring the decrease of bromine color at 380 nm. The reaction was carefully studied and optimized. Under optimum conditions, the stoichiometry and the order of the reaction were determined.The initial rate and fixed time methods were utilized for the determination of salbutamol sulfate concentrations. The AAS method was depended on the oxidation of iron (II) with excess bromine from the bromination reaction of the drug. A new method for separation iron (III) was used. Then iron (II) in aqueous layer was aspirated into air-acetylene flame and determined by AAS. The linear ranges for the proposed methods were 2.0-10.0 and 0.2-2.0 µgmL -1 with detection limit of 0.30 and 0.012 µgmL -1 for kinetic and AAS, respectively. The proposed methods were validated; the mean recovery ranges from 98.0 to 102.0% with RSD < 2.1%. Common excipients did not interfere the measurements of SLS. The methods were successfully applied to determined SLS in dosage forms; there was no significant difference between the proposed methods and official one.

Determination of Salbutamol Sulfate in pharmaceutical formulation with differential pulse voltammetry using poly(Benzofuran-2-Boronic acid) modified platinum electrode

“…For these potential side effects, detection of the SBS in body fluids and urine is very important. Spectrophotometric techniques [4][5][6][7][8], gas chromatography-mass spectrometry [9,10], high performance liquid chromatography [11,12], liquid chromatography-mass spectrometry [13][14][15], capillary electrophoresis [16][17][18][19][20] and chemiluminescence [21,22] are used for the determination of salbutamol in different samples. These techniques need costly equipment, competent personnel and require complex sample pretreatment procedure.…”