Development of a rapid and simple HPLC-UV method for determination of gallic acid in Schinopsis brasiliensis

Abstract

Revista Brasileira de Farmacognosia 25 (2015) 208-211 w w w . s b f g n o s i a . o r g . b r / r e v i s t a a b s t r a c tThe aim of this work was to develop and validate an analytical method for the identification of the chemical marker of Schinopsis brasiliensis Engl., Anacardiaceae. It would determine the total polyphenols and flavonoid content by spectrophotometric methodology in the dried extract of plant. The chromatographic profiles of S. brasiliensis were determined using HPLC-UV. The liquid chromatography method was conducted on a Phenomenex Gemini NX C18 column (250 × 4.6 mm, 5 m). The mobile phase consisted of 0.05% orthophosphoric acid: methanol. The flow rate was 1 ml/min and effluents were monitored at 271 nm. The retention time for gallic acid was 8.5 min. The described method has the advantage of being both rapid and easy. Hence it can be applied for routine quality control analysis of herbal preparation containing S. brasiliensis.

Isolation of antioxidant phenolics from Schinopsis brasiliensis based on a preliminary LC-MS profiling

“…Previous phytochemical investigations on S. brasiliensis leaves reported the presence of tannins and polyphenols, such as methyl gallate, gallic acid, ellagic acid derivatives (Fernandes et al, 2015), and flavonoids (Cardoso et al, 2015). Phytochemical investigation of the MeOH extract of S. brasiliensis stems showed the presence of a biflavonoid and a chalcone (Cardoso et al, 2015), while from S. brasiliensis bark an alkyl phenol along with a steroid derivative were isolated (Cardoso et al, 2005).…”

“…is a plant belonging to the Anacardiaceae family, known as "baraúna", "br auna" or "quebracho", and characterized by arboreal habitat, with dimensions close to 15 m high. It can be found in the Brazilian semi-arid regions and it is a native species of the Caatinga and Atlantic forest (Fernandes et al, 2015). This plant is considered by the "First National Report for the Convention on Biological Diversity" (CBD) as an endangered species, due to the extensive utilisation of its wood in general construction (Cardoso et al, 2005).…”

Quantification of Andrographolide Isolated from <i>Andrographis paniculata</i> Nees Obtained from Traditional Market in Yogyakarta Using Validated HPLC

“…it was determined that the method performs within acceptable limits between 80% and 120%. The accuracy test through recovery of the matrix is crucial to the development of methodologies for herbal products [24]. The results and the average mean of recovery data for each level of marker were within the accepted range as shown in Table 3.…”

Development and validation of an analytical method for quantification of total flavonoids in Alternanthera brasiliana by ultraviolet-visible absorption spectrophotometry

“…The following parameters were evaluated: selectivity, linearity (working range), precision (repeatability, intermediate precision and reproducibility), limit of detection (LOD), limit of quantification (LOQ), accuracy and robustness. All analyzes were performed in triplicate and the reliability of the parameters was verified by the relative standard deviation (RSD%) (Fernandes et al, 2015;Hollands et al, 2017). Only the procedure with complexation was validated considering that the procedure without complexation does not satisfactorily quantify the total flavonoids in A. brasiliana extract.…”