Development of an achiral supercritical fluid chromatography method with ultraviolet absorbance and mass spectrometric detection for impurity profiling of drug candidates. Part II. Selection of an orthogonal set of stationary phases

Lemasson, Elise; Bertin, Sophie; Hennig, Philippe; Boiteux, Hélène; Lesellier, Éric; West, Caroline

doi:10.1016/j.chroma.2015.07.035

Cited by 33 publications

(7 citation statements)

References 22 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…To ensure the authenticity of the materials utilized within this study, the 31 synthesized samples were structurally characterized by 1 H-NMR, 13 C{ 1 H}-NMR, GC-MS and ATR-FTIR and the purity of all samples was confirmed to be >99.5% (by NMR) in all cases. Selectivity in SFC can be controlled by changing the mobile phase composition, temperature and pressure [23][24][25], however, the stationary phase was reported to be the most influential parameter in affecting chromatographic selectivity [26][27][28]. Hence, a generic gradient SFC condition that is typically used in screening activities was employed [26,27,29] to assess the chromatographic retention and separation of 1 -31 on a range of disparate SFC stationary phases.…”

Section: Resultsmentioning

confidence: 99%

Guilty by dissociation: Part B: evaluation of Supercritical Fluid Chromatography (SFC-UV) for the analysis of regioisomeric diphenidine-derived Novel Psychoactive Substances (NPS)

Cochrane

Field

Hulme

et al. 2022

Journal of Pharmaceutical and Biomedical Analysis

View full text Add to dashboard Cite

Section: Resultsmentioning

confidence: 99%

Guilty by dissociation: Part B: evaluation of Supercritical Fluid Chromatography (SFC-UV) for the analysis of regioisomeric diphenidine-derived Novel Psychoactive Substances (NPS)

Cochrane

Field

Hulme

et al. 2022

Journal of Pharmaceutical and Biomedical Analysis

View full text Add to dashboard Cite

“…A possible explanation might be changes in column selectivity due to a divergent surface silanol activity, e.g. caused by polar endcapping . The addition of diethylamine as well as 50% of acetonitrile in methanol was advantageous because the resolution of 4 and 5 could be significantly improved (Fig.…”

Section: Resultsmentioning

confidence: 99%

Fast and improved separation of major coumarins inAmmi visnaga(L.) Lam. by supercritical fluid chromatography

2016

View full text Add to dashboard Cite

The first supercritical fluid chromatography method for the determination of five major coumarins (dihydrosamidin, visnadin, samidin, khellin, and visnagin) in Ammi visnaga fruits is described. Their baseline separation was possible in less than 5 min by using a UPC HSS C SB column with 1.8 μm particle size and a mobile phase comprising CO , methanol, acetonitrile, and diethylamine. The type of stationary phase used was of particular relevance because, except for the selected one, the others did not resolve the two structural isomers dihydrosamidin and visnadin. Method validation confirmed that the procedure is linear (R ≥ 0.9996) in a concentration range from 6 to 480 μg/mL, it is accurate (recovery rates: 97.2-103.6%) and precise (intraday deviation ≤ 6.6%, intraday deviation ≤ 1.7%); injecting 1 μL of standard solution, the determined limit of detection was below 1.9 μg/mL for all compounds. The analysis of different A. visnaga samples revealed their similar compositions, and khellin (0.75-1.01%) and visnagin (0.18-0.46%) were the dominant coumarins. Visnadin and dihydrosamidin, the individual quantification of which is described for the first time, were present at concentrations below 0.14%.

show abstract

“…As discussed earlier, even under SFC conditions, SPP columns exhibit increased separation power over TPP, producing faster separations with higher efficiencies. Some applications were published, including drug discovery (Lemasson et al, 2015;West et al, 2018) and metabolomics (Desfontaine et al, 2018).…”

Section: Spp For Achiral Separations In Sfcmentioning

confidence: 99%

Fundamental to achieving fast separations with high efficiency: A review of chromatography with superficially porous particles

Luo

DeStefano

Langlois

et al. 2021

Biomedical Chromatography

View full text Add to dashboard Cite

Types of particles have been fundamental to LC separation technology for many years. Originally, LC columns were packed with large-diameter (>100 μm) calcium carbonate, silica gel, or alumina particles that prohibited fast mobile-phase speeds because of the slow diffusion of sample molecules inside deep pores. During the birth of HPLC in the 1960s, superficially porous particles (SPP, ≥30 μm) were developed as the first high-speed stationary-phase support structures commercialized, which permitted faster mobile-phase flowrates due to the fast movement of sample molecules in/out of the thin shells. These initial SPPs were displaced by smaller totally porous particles (TPP) in the mid-1970s. But SPP history repeated when UHPLC emerged in the 2000s. Stationary-phase support structures made from sub-3-μm SPPs were introduced to chromatographers in 2006. The initial purpose of this modern SPP was to enable chromatographers to achieve fast separations with high efficiency using conventional HPLCs. Later, the introduction of sub-2-μm SPPs with UHPLC instruments pushed the separation speed and efficiency to a very fast zone.This review aims at providing readers a comprehensive and up-to-date view on the advantages of SPP materials over TPPs historically and theoretically from the material science angle.

show abstract

Development of an achiral supercritical fluid chromatography method with ultraviolet absorbance and mass spectrometric detection for impurity profiling of drug candidates. Part II. Selection of an orthogonal set of stationary phases

Cited by 33 publications

References 22 publications

Guilty by dissociation: Part B: evaluation of Supercritical Fluid Chromatography (SFC-UV) for the analysis of regioisomeric diphenidine-derived Novel Psychoactive Substances (NPS)

Guilty by dissociation: Part B: evaluation of Supercritical Fluid Chromatography (SFC-UV) for the analysis of regioisomeric diphenidine-derived Novel Psychoactive Substances (NPS)

Fast and improved separation of major coumarins inAmmi visnaga(L.) Lam. by supercritical fluid chromatography

Fundamental to achieving fast separations with high efficiency: A review of chromatography with superficially porous particles

Contact Info

Product

Resources

About