Development of bioabsorbable polylactide membrane with controllable hydrophilicity for adjustment of cell behaviours

Abstract

Cell functions can be mediated through their interactions with the microenvironments, which highly depend on the surface state of the substrate. However, how to finely adjust the surface of biomaterials is still very challenging. In this study, poly(d,l-lactide) (PDLLA) with high molecular weight was synthesized via ring opening polymerization, which was hot-pressed into PDLLA membrane. In order to modify the hydrophobicity of the membrane (a limiting factor for its biomedical application), an amphiphilic monomethoxyl poly(ethylene glycol)-b-poly(d,l-lactide) (PEG-PDLLA) was selected to improve its surface hydrophilicity through a simple self-assembly approach. It was found that the contact angles of the modified membrane can be well controlled by variation of PEG-PDLLA concentrations. In vitro cell biological study indicates that optimized cell adhesion can be achieved on the modified membrane with a contact angle of around 50° via its self-assembly with an ethanol/water solution of PEG-PDLA (35 mg ml−1). The surface modification of the membrane also changed its biodegradation property in the process of its incubation period up to 240 days. The surface modification method may afford an effective way for adjustment of the surface (interface) of membrane (scaffolds) of different biomaterials, beyond polylactide.

Electrospun metformin-loaded polycaprolactone/chitosan nanofibrous membranes as promoting guided bone regeneration membranes: Preparation and characterization of fibers, drug release, and osteogenic activity in vitro

“…Studies have shown that membranes with a WCA ranging from 40° to 60° may be appropriate for cell adhesion, 49 and optimized cell adhesion can be achieved on the modified membrane with a contact angle of around 50°. 50,51 In our study, the results of cell proliferation and morphology indicated that the obtained C-PCL/CS/MET membranes with a WCA of 44.25 ± 1.37° would be more suitable for BMSCs growth.…”

Incorporating redox-sensitive nanogels into bioabsorbable nanofibrous membrane to acquire ROS-balance capacity for skin regeneration

“…1 a). PLLA offered a chemical shift at δ = 1.56 ppm corresponding to methyl group on the polymer chain, and a chemical shift at δ = 5.16 ppm related to the methylene group on the polymer framework, indicating a successful synthesis of PLLA polymer [ 32 ]. Gel penetration chromatography (GPC) is a common method for molecular weight measurement of polymers.…”

“…The surface characteristics of materials, including surface hydrophilicity and charges, have a significant impact on cell behaviors [ 39 , 40 ]. In this case, the hydrophobicity of PLLA polymer greatly limits its biomedical application [ 32 ]. The hydrophilicity of PLLA and PNA-doped nanofibrous membranes was evaluated using a water contact angle test at room temperature.…”

“…As shown in Fig. S2 , the incorporation of PNA nanogels accelerated the degradation speed of the membrane, probably because of the higher hydrophilicity of the PNA modified membranes which enhanced their water adsorption ability and erosion capacity [ 32 ] ( Fig. 3 d).…”

Effects of polyethylene glycol content on the properties of a silk fibroin/nano-hydroxyapatite/polyethylene glycol electrospun scaffold

“…Again, cell adhesion and proliferation were found to t into the scaffold of PEG-1, but not that of PEG-2. It has been suggested that scaffolds are conducive to cell adhesion when the contact angle ranges from 40 to 60 , 34,35 and the water contact angle of the PEG-2 group was outside that range.…”