Development of black rot resistant interspecific hybrids between Brassica oleracea L. cultivars and Brassica accession A 19182, using embryo rescue

Tonguç, Muhammet; Griffiths, Phillip D.

doi:10.1023/b:euph.0000032733.47031.5f

Cited by 43 publications

(30 citation statements)

References 18 publications

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“…The best option for organic production would be the use of resistance. Incomplete resistance is found in B. oleracea, but more complete forms of resistance have been identified in B. napus and B. carinata [93]. Early attempts to introduce resistance from B. carinata into B. oleracea were made using somatic hybridization, and recently in vitro embryo culture was used to introgress resistance [93].…”

Section: Resistance To Major Seed-borne Diseasesmentioning

confidence: 99%

The need to breed crop varieties suitable for organic farming, using wheat, tomato and broccoli as examples: A review

Bueren

Jones

Tamm

et al. 2011

NJAS: Wageningen Journal of Life Sciences

326

209

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a b s t r a c tIt is estimated that more than 95% of organic production is based on crop varieties that were bred for the conventional high-input sector. Recent studies have shown that such varieties lack important traits required under organic and low-input production conditions. This is primarily due to selection in conventional breeding programmes being carried out in the background of high inorganic fertilizer and crop protection inputs. Also, some of the traits (e.g., semi-dwarf genes) that were introduced to address problems like lodging in cereals in high-input systems were shown to have negative side-effects (reduced resistance to diseases such as Septoria, lower protein content and poorer nutrient-use efficiency) on the performance of varieties under organic and low-input agronomic conditions. This review paper, using wheat, tomato and broccoli as examples, describes (1) the main traits required under low-input conditions, (2) current breeding programmes for organic, low-input agriculture, (3) currently available breeding and/or selection approaches, and (4) the benefits and potential negative side-effects of different breeding methodologies and their relative acceptability under organic farming principles.

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Section: Resistance To Major Seed-borne Diseasesmentioning

confidence: 99%

The need to breed crop varieties suitable for organic farming, using wheat, tomato and broccoli as examples: A review

Bueren

Jones

Tamm

et al. 2011

NJAS: Wageningen Journal of Life Sciences

326

209

View full text Add to dashboard Cite

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“…Interspecific hybridization between B. juncea and B. oleracea has been used to synthesize bridging materials for transferring desirable traits between Brassica species (Arumugam et al 1996;Tonguc and Griffiths 2004;Chen et al 2011). In this study, the AABCC and ABCC hybrids were synthesized as bridges for introducing yellow seed traits from B. juncea and B. oleracea to B. napus.…”

Section: Discussionmentioning

confidence: 99%

Characterization of interploid hybrids from crosses between Brassica juncea and B. oleracea and the production of yellow-seeded B. napus

Wen

Zhu

et al. 2012

Theor Appl Genet

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Yellow-seeded Brassica napus was for the first time developed from interspecific crosses using yellow-seeded B. juncea (AABB), yellow-seeded B. oleracea (CC), and black-seeded artificial B. napus (AACC). Three different mating approaches were undertaken to eliminate B-genome chromosomes after trigenomic hexaploids (AABBCC) were generated. Hybrids (AABCC, ABCC) from crosses AABBCC × AACC, AABBCC × CC and ABCC × AACC were advanced by continuous selfing in approach 1, 2 and 3, respectively. To provide more insight into Brassica genome evolution and the cytological basis for B. napus resynthesis in each approach, B-genome chromosome pairing and segregation were intensively analyzed in AABCC and ABCC plants using genomic in situ hybridization methods. The frequencies at which B-genome chromosomes underwent autosyndesis and allosyndesis were generally higher in ABCC than in AABCC plants. The difference was statistically significant for allosyndesis but not autosyndesis. Abnormal distributions of B-genome chromosomes were encountered at anaphase I, including chromosome lagging and precocious sister centromere separation of univalents. These abnormalities were observed at a significantly higher frequency in AABCC than in ABCC plants, which resulted in more rapid B-genome chromosome elimination in the AABCC derivatives. Yellow or yellow-brown seeds were obtained in all approaches, although true-breeding yellow-seeded B. napus was developed only in approaches 2 and 3. The efficiency of the B. napus construction approaches was in the order 1 > 3 > 2 whereas this order was 3 > 2 > 1 with respect to the construction of yellow-seeded B. napus. The results are discussed in relation to Brassica genome evolution and the development and utilization of the yellow-seeded B. napus obtained here.

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“…Therefore, we assume that a specific hybridization interaction-expressed in the ovule response to the tissue culture conditions, particularly when we compared the ovule response at 30 DAP among the three crosses-exists between the different accessions used in this study. Tonguç and Griffiths (2004) reported that the genotype of parental lines influenced the outcomes of embryo rescue and the production of interspecific hybrid plants between crosses of different cultivars of Brassica juncea and B. oleracea, a cross that naturally never set viable seeds. Clarke et al (2006) reported that the genotype used in the cross also played a significant role in the ability of immature embryos to germinate in vitro following interspecific hybridization between Cicer In the immature embryos, responses did not differ significantly between sucrose concentrations, but the rate of embryo conversion and total number of plantlets regenerated was lower than in 0.17 M sucrose.…”

Section: Discussionmentioning

confidence: 99%

“…Clarke et al (2006) reported that the genotype used in the cross also played a significant role in the ability of immature embryos to germinate in vitro following interspecific hybridization between Cicer In the immature embryos, responses did not differ significantly between sucrose concentrations, but the rate of embryo conversion and total number of plantlets regenerated was lower than in 0.17 M sucrose. Rescuing small, intact embryos during their early developmental stages is technically intractable and time-consuming, and therefore fertilized ovules or ovaries are increasingly being viewed as part of a viable, alternative procedure, as was reported for Cicer arietinum (Ahmad and Slinkard 2003;Clarke et al 2006), Brassica oleracea (Tonguç and Griffiths 2004), Lens culinaris (Fratini and Ruiz 2006), Cucumis melo (Nuñez-Palenius et al 2006), and Lilium longiflorum (Wang et al 2009), to name but a few species. However, for embryos with high dormancy levels or that lost the generative capacity to selfproduce a plant, immature embryo rescue, when it is technically feasible, is a practical rescue alternative that has been successfully applied in Leymus chinensis (Liu et al 2004), Trifolium alexandrinum 9 T. resupinatum (Kaushal et al 2005), and Diospyros kaki (Leng and Yamamura 2006).…”

Section: Discussionmentioning

confidence: 99%

Creation of novel interspecific-interploid Hylocereus hybrids (Cactaceae) via embryo rescue

2012

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In this study we developed a technique for rescuing embryos at a very early developmental stage following interspecific-interploid crosses between Hylocereus sp. Controlled hand pollinations were performed between the tetraploid H. megalanthus (Vaup.) Bauer as the female parent and either diploid H. monacanthus (Lem.) Britton et Rose or H. undatus (Haw.) Britton et Rose as the male parent. The fertilized ovules were excised from ovaries 10 or 30 days after pollination (DAP). Pollinated ovules containing the funiculi and placental tissue and immature embryos were placed on half-strength basal Murashige and Skoog (MS) medium containing 680 lM glutamine, 0.54 lM a-naphthaleneacetic acid, and 0.45 lM thidiazuron and supplemented with 0.00, 0.09, 0.17 or 0.26 M sucrose concentrations. The best ovule response was recorded at 30 DAP, and the most prolific callus formation was observed at 10 DAP. Callus formation was observed in most of the treatments using whole ovules but not in the isolated immature embryos. The calli were mucilaginous or compact, transparent and friable, but they did not form embryogenic structures. Embryo development was significantly affected by the sucrose concentration, and the best results were obtained with 0.17 M sucrose. More than 70 % of the obtained hybrids were successfully hardened off and transplanted in soil where they grew normally. Ploidy level analyses of 77 putative hybrids exposed diploid, triploid, tetraploid, and higher than tetraploid levels. Among those hybrids studied, 22 progenies were randomly chosen for amplified fragment length polymorphism analysis, and all were identified as genuine hybrids. The technology described here is an additional stage in the breeding program for Hylocereus species resulting in novel, interspecific hybrids obtained using the embryo rescue technique. Keywords Amplified fragment length polymorphism (AFLP) Á Flow cytometry Á a-Naphthaleneacetic acid Á Ovule culture Á Thidiazuron Á Vine cacti Abbreviations AFLP Amplified fragment length polymorphism BGU Ben-Gurion University of the Negev DAP Days after pollination IE Immature embryos MS Murashige and Skoog NAA a-Naphthaleneacetic acid NIB Nuclei isolation buffer TDZ Thidiazuron WO Whole ovules

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Development of black rot resistant interspecific hybrids between Brassica oleracea L. cultivars and Brassica accession A 19182, using embryo rescue

Cited by 43 publications

References 18 publications

The need to breed crop varieties suitable for organic farming, using wheat, tomato and broccoli as examples: A review

The need to breed crop varieties suitable for organic farming, using wheat, tomato and broccoli as examples: A review

Characterization of interploid hybrids from crosses between Brassica juncea and B. oleracea and the production of yellow-seeded B. napus

Creation of novel interspecific-interploid Hylocereus hybrids (Cactaceae) via embryo rescue

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