Development of early mouse and sheep embryos in serum-supplemented, phosphate-buffered saline

Ladd, P.C.; Plair, A.C.; Braidlow, B.K.; Snyder, Diana

doi:10.1016/0093-691x(82)90138-8

Cited by 3 publications

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“…Serum contains a heterogeneous mixture of naturally occurring substances, including many different proteins. The composition of different batches of serum from the same species may vary widely (Taylor 1974;Blunt 1975), as may the ability of different batches of serum to support embryo development in vitro (Ladd et al 1982;Bates et al 1985;Shirley et al 1985). Some types or batches of serum inhibit embryo development in vitro (Caro and Trounson 1984;Shirley et al 1985).…”

Section: Introductionmentioning

confidence: 99%

Development of Sheep Embryos in Vitro in a Medium Supplemented with Different Serum Fractions

Batt

Miller²

1988

Aust. Jnl. Of Bio. Sci.

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Sheep embryos will generally develop into expanded blastocysts in vitro only in culture media supplemented with serum or serum components. In order to better understand how serum supports embryo development, a batch of ovine serum was fractionated by (a) ultrafiltration into two components containing substances with molecular weights greater and less than 10 Kd (kilodaltons), and (b) gel filtration into protein fractions 1, 2 and 3, containing groups of proteins with mean molecular weights of about 500, 150 and 65 Kd, respectively. The principal protein in fraction 3 was albumin. Day 6 sheep morulae were cultured in vitro for 48 hours in a bicarbonate-buffered salt solution supplemented with various concentrations of ovine serum or of these components or protein fractions of serum.Morulae could develop to fully expanded blastocysts in medium supplemented with whole serum or with the> 10 Kd component or protein fraction 3 only, but could not develop in medium supplemented with the < 10 Kd component only or with the < 10 Kd component and protein fractions 1 or 2. However, the proportion of embryos that developed fully in medium supplemented with the > 10 Kd component or protein fraction 3 was increased by adding the < 10 Kd component of serum to the medium. The addition of protein fraction 2 decreased the proportion of embryos that developed to expanded blastocysts in medium containing protein fraction 3 and the < 10 Kd component, but not in medium containing whole serum. Since the compositions of different sera may vary markedly, these results suggest (a) reasons why different sera vary in their ability to support embryo development in vitro, and (b) factors which may influence development of the sheep embryo in the uterus, where plasma proteins comprise nearly all the protein in the fluid bathing the embryo.

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Section: Introductionmentioning

confidence: 99%

Development of Sheep Embryos in Vitro in a Medium Supplemented with Different Serum Fractions

Batt

Miller²

1988

Aust. Jnl. Of Bio. Sci.

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Development of screening systems for evaluation of materials used in mammalian embryo transfer

Lee

Boone²,

Brackelsberg

et al. 1988

Theriogenology

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Oxygen concentration and protein source affect the development of preimplantation goat embryos in vitro

Batt¹,

Gardner²,

Cameron³

1991

Reprod. Fertil. Dev.

142

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The effect of oxygen concentration and the source of protein in culture medium on the development of 2- to 4-cell goat embryos in vitro was investigated. Embryos were collected from superovulated Angora-Cashmere-cross goats 48 h after ovulation and cultured for 6 days in synthetic oviduct fluid (SOF) medium under one of two oxygen concentrations (20% or 7%) and in the presence of one of five protein sources; Miles bovine serum albumin (Miles BSA), Commonwealth Serum Laboratory bovine serum albumin (CSL BSA), goat serum (GS), fetal calf serum (FCS) and human serum (HS). In the presence of 20% oxygen the percentage of embryos reaching the expanded and/or hatched blastocyst stage in SOF medium containing Miles BSA was 29%, with a mean cell number per embryo of 28.1 +/- 6.0 (+/- s.e.m.). Use of an oxygen concentration of 7% significantly increased the percentage of embryos reaching this stage (80%, P less than 0.01) and the mean number of cells per embryo (65.3 +/- 8.2, P less than 0.01). The mean number of cells of the early-cleavage-stage embryos was significantly lower when the medium contained CSL BSA, GS or FCS (42.7 +/- 5.6, 29.0 +/- 6.1 and 21.3 +/- 3.2, respectively) than with Miles BSA (92.8 +/- 6.4) or HS (104.8 +/- 17.2) (P less than 0.01). Under 7% oxygen and with Miles BSA or HS, embryos were morphologically comparable to those developed in vivo, but the mean cell numbers in vitro were only approximately half those obtained in vivo.

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Development of early mouse and sheep embryos in serum-supplemented, phosphate-buffered saline

Cited by 3 publications

References 0 publications

Development of Sheep Embryos in Vitro in a Medium Supplemented with Different Serum Fractions

Development of Sheep Embryos in Vitro in a Medium Supplemented with Different Serum Fractions

Development of screening systems for evaluation of materials used in mammalian embryo transfer

Oxygen concentration and protein source affect the development of preimplantation goat embryos in vitro

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