Development of ELISA Exploring Recombinant Variable Surface Glycoprotein for Diagnosis of Surra in Animals

Seroprevalence of Trypanosoma evansi in cattle and analysis of associated climatic risk factors in Mizoram, India

“…Two serological tests, both CATT and iELISA employed in the present study gave similar results with respect to seropositivity as reported earlier (Sengupta et al 2016). As CATT/T.evansi mainly detects parasite specific IgM antibodies, it can be used to detect recent infection whereas ELISA can be used to identify latent infections as it detects specific IgG antibodies.…”

“…Yeast (X-33 P PICZa, TEVSG) (Sengupta et al 2016) glycerol stock culture stored at -80 °C was revived using Yeast Extract-Peptone-Dextrose (YPD) media with zeocin (100 lg/ml) until adequate turbidity was observed. To induce the gene expression, the inoculum was subcultured intobuffered glycerolcomplex medium (BMGY, Invitrogen)and incubated for 16-18 h at 28 °C at 150 rpm(OD 600 = 4).…”

“…The ELISA was performed using standard protocol with an in-house developed and validated test (Sengupta et al 2016)with necessary modifications to detect the presence of antibodies against T.evansi in serum samples. In brief, microtiter plates (Maxisorp Ò , Nunc) were coated with 1.5 lg/well ofpurified recombinant Variable Surface Glycoprotein (r-VSG)in Carbonate-Bicarbonate Buffer (CBB) and incubated overnight at 4 °C.…”

“…The CATT/T.evansi remains as the gold standard test for detection of surra as per Office International des Epizooties (OIE) but the sensitivity of the test is compromised (Songa and Hamers 1988). Recombinant protein based indirect ELISA has been widely used for detection of the carrier animals (Rudramurthy et al 2018;Sengupta et al 2014Sengupta et al , 2016. These tests can be used for conducting epidemiological studies and also to monitor the effective implementation of control strategies against trypanosomosis.…”