Development of enzyme immunoassay for the detection of the herbicide norflurazon

Riggle, Bruce D.; Dunbar, Bohn

doi:10.1021/jf00100a009

Cited by 21 publications

(6 citation statements)

References 10 publications

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“…This could be due to a nonspecific recognition of the Thyr-hapten conjugates. Our sensitivity results are of the same order as those obtained with ELISA of two herbicides (norflurazon and bromacil), described by Riggles et al (1990) and Szurdoki et al (1992). Indeed, they obtained IC 50 values between 1 and 10 ng/mL, that is, 1-10 µg/L or 1-10 ppb, as we did.…”

Section: Resultssupporting

confidence: 87%

“…Haptens 8-12, 20, and 21 were coupled either to BSA (for injection to rabbits) or to Thyr (for coating). The conjugation procedure was adapted from those of Riggles et al (1990) and Szurdoki et al (1992). Briefly, 0.072 mmol of tributylamine (17.3 µL), 0.036 mmol of isobutyl chloroformate (4.7 µL), and 0.036 mmol of hapten were dissolved in 1 mL of DMF at 4 °C and then stirred on an ice bath for 30 min.…”

Section: -(2-carboxyethyl)-7-hydroxy-3-(4-hydroxyphenyl)-4hchromen-4-...mentioning

confidence: 99%

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Synthesis of Haptens and Conjugates for ELISAs of Phytoestrogens. Development of the Immunological Tests

Bennetau-Pelissero

Houerou

Lamothe

et al. 2000

J. Agric. Food Chem.

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Seven carboxylic acid haptens of isoflavonoids were synthesized, with the spacer arm on the oxygen atom at the C7 position for one series, with formononetin, daidzein, equol, biochanin A, and genistein, and at the C8 position for a second series, with only formononetin and daidzein. The different haptens were coupled to bovine serum albumin (BSA) and to swine thyroglobulin (Thyr). Polyclonal antibodies were generated against the BSA conjugates. Enzyme-linked immunosorbent assays (ELISAs) were developed based on competition between free phytoestrogens and the Thyr-hapten conjugates for specific antibodies. IC(50) values of the standard curves ranged between 0.8 and 20 ng/mL that is, 0.3 and 9.2 pmol/well. The antibodies obtained should be useful for assays in vegetable matter as well as in biological fluids after a separation step. These ELISAs should be valuable also in the food industry to control phytoestrogen concentrations prior to and after processing.

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Section: Resultssupporting

confidence: 87%

Section: -(2-carboxyethyl)-7-hydroxy-3-(4-hydroxyphenyl)-4hchromen-4-...mentioning

confidence: 99%

Synthesis of Haptens and Conjugates for ELISAs of Phytoestrogens. Development of the Immunological Tests

Bennetau-Pelissero

Houerou

Lamothe

et al. 2000

J. Agric. Food Chem.

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“…Antisera dilu tion of 1:50 and 1:200 is less than most other pesticide immunoassays, with typical dilutions of 1:500 to > 1: 20 000. Detections of < 1 ng analyte per ml have been attained with other immunoassays (3,19). EPTC speci ficity in the present ELISA system might be improved by injection of different rabbits with the same antigen, as antibody response varies among individual animals.…”

Section: Resultsmentioning

confidence: 97%

Immunoassay to Detect Enhanced Carbamothioate Degradation in Soil

et al. 1993

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An indirect enzyme-linked immunosorbent assay (ELISA) for EPTC was developed that may be useful for predicting enhanced carbamothioate herbicide degradation in adapted soils. A modified radioimmunoassay (RIA) procedure was used to detect antibodies specific for EPTC and establish antibody dilutions for optimum ELISA sensitivity. Antibodies were obtained from a rabbit injected with a butylate-analog protein conjugate. An indirect ELISA subsequently was developed for EPTC with a detection range of 10 to 1280 ng ml−1 in a 1:3 aqueous dilution of a 90:10 (v/v) acetonitrile (ACN):water soil extract. EPTC recovery from soil was > 96%, as determined by conventional gas chromatography. Measurement of low concentrations of residual EPTC with the ELISA was less quantitative than by gas chromatography, but the immunoassay provided a rapid, potentially cost-effective method for screening soils to determine enhanced carbamothioate degradation.

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“…Pyridazinone scaffolds are a series of potent bioactive molecules applied in medicine and agriculture. Among them, 3(2 H )-pyridazinone derivatives are extensively developed in crop protection, such as insecticide/acaricide, 21 herbicide, 22 plant growth regulator, 23 and fungicides, 24 while there is no application as plant activators.…”

mentioning

confidence: 99%