Development of ssDNA aptamers for the capture and detection of Salmonella typhimurium

Moon, Ji-Hea; Kim, Gi‐Young; Park, Saetbyeol

doi:10.1039/c4ay01035c

Cited by 19 publications

(10 citation statements)

References 30 publications

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“…by exploiting the surface plasmon resonance features of GNPs. These methods either employ GNPs bound to thiolated DNA or to aptamers for sensitive and selective detection of this deadly pathogen . A new multiplex method for simultaneous detection of three pathogenic bacteria ( S. typhimurium , S. aureus , and Vibrio parahaemolyticus ) has also been demonstrated using aptamer‐conjugated luminescent upconversion nanoparticles .…”

Section: Nanobiosensors For Foodborne Microbial Pathogensmentioning

confidence: 99%

Moving forward in plant food safety and security through NanoBioSensors: Adopt or adapt biomedical technologies?

et al. 2015

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Plant-based foods are integral part of our day-to-day diet. Increasing world population has put forth an ever increasing demand for plant-based foods, and food security remains a major concern. Similarly, biological, chemical, and physical threats to our food and increasing regulatory demands to control the presence of foreign species in food products have made food safety a growing issue. Nanotechnology has already established its roots in diverse disciplines. However, the food industry is yet to harness the full potential of the unique capabilities offered by this next-generation technology. While there might be safety concerns in regards to integration of nanoproducts with our food products, an aspect of nanotechnology that can make remarkable contribution to different elements of the food chain is the use of nanobiosensors and diagnostic platforms for monitoring food traceability, quality, safety, and nutritional value. This brings us to an important question that whether existing diagnostic platforms that have already been well developed for biomedical and clinical application are suitable for food industry or whether the demands of the food industry are altogether different that may not allow adoption/adaptation of the existing technology. This review is an effort to raise this important "uncomfortable" yet "timely" question.

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Section: Nanobiosensors For Foodborne Microbial Pathogensmentioning

confidence: 99%

Moving forward in plant food safety and security through NanoBioSensors: Adopt or adapt biomedical technologies?

et al. 2015

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“…With this aptamer, the detection range of 50-10 6 cfu ̸mL and LOD of 5 cfu ̸mL were obtained (Duan et al 2013c). These approaches could be employed to develop an easy, rapid, and sensitive methodology for other closely related pathogen detection in food(s) as only aptamerAuNPs based colorimetric method can detect 10 6 cfu/mL (Moon et al 2014).…”

Section: Salmonella Speciesmentioning

confidence: 99%

“…2) where bound sequences are perfectly separated from unbound ones. Moon and others reported that the use of cell sorting followed by FAM-labeling of aptamer candidates improved the affinity up to a Kd of 3.49 ± 1.43 nM (Moon et al 2014).…”

mentioning

confidence: 99%

An Update on Aptamer-Based Multiplex System Approaches for the Detection of Common Foodborne Pathogens

et al. 2017

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Foodborne ailments constitute a public health challenge and pose an incredible economic burden in healthcare system around the globe. This dilemma has urged authorities and other entities working in field of food quality control and supply chain to play a pivotal role in ensuring food safety. Analytical strategies have been developed using numerous systematic evolution of ligands by exponential enrichment (SELEX) methods to assure food safety. High-affinity and high-sensitivity ssDNA and RNA aptamers against pathogens have emerged as a novel strategy, as compared to the more resource-demanding and complicated biochemical test-based approaches. Thus, this review aims to focus on some methods used in the selection of specific bare, modified, and conjugated aptamers and on the further analysis of selected aptamers using flow cytometer or post-SELEX modifications for enhanced detection of frequently diagnosed foodborne bacteria such as Bacillus sp., Campylobacter jejuni, Escherichia sp., Salmonella sp., Staphylococcus aureus, Shigella sp., Listeria monocytogenes, and Streptococcus pyogenes and/or targeting their cell components towards attaining fast, sensitive, and selective methods for the detection of pathogens in food(s) or other sources.

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“…SELEX-generated aptamers are easier, cheaper, more stable, less immunogenic, allow better synthesis, and exhibit higher productivity than antibodies (Lakhin et al, 2013;Robati et al, 2018;Wu et al, 2012a). Recently, several aptamers have been generated against various targets, such as toxin (Mondal et al, 2018), protein (Hu et al, 2014), antibiotics (He et al, 2013;Song et al, 2011), and whole-cell pathogens (Bayrac¸et al, 2017;Duan et al, 2013aDuan et al, , 2013bDuan et al, , 2016Moon et al, 2014;Suh and Jaykus, 2013;Tianjun et al, 2016;Wu et al, 2012aWu et al, , 2012bYuan et al, 2014b).…”

Section: Introductionmentioning

confidence: 99%

Colorimetric aptasensor for detecting Salmonella spp., Listeria monocytogenes, and Escherichia coli in meat samples

Ledlod

Areekit

Santiwatanakul

et al. 2020

Food sci. technol. int.

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In this study, we successfully developed a simple and rapid method for simultaneous detection of Salmonella spp., Listeria monocytogenes, and Escherichia coli using gold nanoparticles and the aptamer aptasensor. We screened 25 specific DNA aptamer candidates against these pathogens using whole-cell Systematic Evolution of Ligands by EXponential enrichment. Among them, Ap6 was selected due to its low energy minimization values of −12.25 and −27.67 kcal/mol derived from MFold and RNAFold analysis, respectively. The assay presented in this study allowed the visual colorimetric detection of labeled colloidal gold nanoparticles as well as determination of UV absorbance at 625 and 525 nm under optimized conditions. The detection limit of this aptasensor was as less as 105 CFU/ml. A random investigation of 50 meat samples, including ham and chicken sausages, collected from the local market revealed 96% accuracy, 96% specificity, and 100% sensitivity of the assay. The colorimetric aptasensor can accomplish one-step detection without pre-culture, DNA extraction, and amplification. Hence, it is an easy, rapid, specific, and qualitative assay that can be used as a point-of-care testing to directly detect multiplex foodborne pathogens.

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Development of ssDNA aptamers for the capture and detection of Salmonella typhimurium

Abstract: There is a global need for methods allowing rapid detection of pathogens in food samples, particularly for methods amenable for use in biosensors.

Cited by 19 publications

References 30 publications

Moving forward in plant food safety and security through NanoBioSensors: Adopt or adapt biomedical technologies?

Moving forward in plant food safety and security through NanoBioSensors: Adopt or adapt biomedical technologies?

An Update on Aptamer-Based Multiplex System Approaches for the Detection of Common Foodborne Pathogens

Colorimetric aptasensor for detecting Salmonella spp., Listeria monocytogenes, and Escherichia coli in meat samples

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