Development of TaqMan real-time PCR assays for monitoring Vibrio harveyi infection and a plasmid harbored by virulent strains in European abalone Haliotis tuberculata aquaculture

Schikorski, David; Renault, Tristan; Paillard, Christine; Bidault-Toffin, Adeline; Tourbiez, Delphine; Saulnier, Denis

doi:10.1016/j.aquaculture.2013.02.005

Cited by 27 publications

(20 citation statements)

References 40 publications

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“…Their purity was verified by real‐time PCR (Schikorski et al . ). BB120 strain was shown to exhibit strong virulence in brine shrimp Artemia franciscana, whereas the use of AI‐2 and CAI‐1‐deficient mutants abolished virulence of V. harveyi to brine shrimp (Defoirdt and Sorgeloos ).…”

Section: Methodsmentioning

confidence: 97%

“…Liquid broth media screening: The strains used for this bioassay are V. harveyi BB120 wild type and its derived double mutants (JAF 375, JMH 597 and JMH 612) obtained from Bassler's laboratory (Bassler et al 1997;Henke and Bassler 2004a,b). Their purity was verified by real-time PCR (Schikorski et al 2013). BB120 strain was shown to exhibit strong virulence in brine shrimp Artemia franciscana, whereas the use of AI-2 and CAI-1-deficient mutants abolished virulence of V. harveyi to brine shrimp (Defoirdt and Sorgeloos 2012).…”

Section: Qsi Screeningsmentioning

confidence: 99%

“…Vibrio harveyi has been identified as a serious pathogen in French Polynesia. This bacterium is known to induce severe economic losses in many vertebrate and invertebrate aquacultured species (Austin and Zhang 2006;Schikorski et al 2013). It is responsible for the septicaemia of the cultured orbicular batfish in Tahiti.…”

Section: Introductionmentioning

confidence: 99%

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Quorum sensing inhibitors from Leucetta chagosensis Dendy, 1863

Mai

Tintillier

Lucasson

et al. 2015

Letters in Applied Microbiology

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A new type of quorum sensing inhibitors was isolated from the sponge Leucetta chagosensis. One of them inhibits strongly the AI-2 channel of Vibrio harveyi, a marine pathogen of special importance in aquaculture. The activity of five different related compounds, including three new natural products discovered there, was investigated leading to structure-activity relationships which are useful for the design of new quorum sensing inhibitors to control marine infectious pathogens.

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Section: Methodsmentioning

confidence: 97%

Section: Qsi Screeningsmentioning

confidence: 99%

See 1 more Smart Citation

Quorum sensing inhibitors from Leucetta chagosensis Dendy, 1863

Mai

Tintillier

Lucasson

et al. 2015

Letters in Applied Microbiology

Self Cite

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“…In addition, for V. harveyi-related species, highly similar genomes and genome plasticity may also limit precise identification by molecular techniques in some cases (Thompson and Swings, 2006;Sawabe et al, 2007). Thompson et al, 2007;Cano-Gomez et al, 2011), loop-mediated isothermal amplification (LAMP) (Cao et al, 2010), and TaqMan PCR to detect potentially virulent strains for H. tuberculata (Schikorski et al, 2014) (Table 2).…”

Section: Harveyi Cladementioning

confidence: 99%

Bacterial diseases in marine bivalves

Travers

Miller

Roque

et al. 2015

Journal of Invertebrate Pathology

146

121

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Bivalve aquaculture is seriously affected by many bacterial pathogens that cause high losses in hatcheries as well as in natural beds. A number of Vibrio species, but also members of the genera Nocardia and Roseovarius, are considered important pathogens in aquaculture. The present work provides an updated overview of main diseases and implicated bacterial species affecting bivalves. This review focuses on aetiological agents, their diversity and virulence factors, the diagnostic methods available as well as information on the dynamics of the host-parasite relationship.

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“…In the current study, we have used and tested a TaqMan probe developed by Schikorski et al (2013) for qPCR assays of V. harveyi (16). Measurements obtained by TaqMan qPCRs following a scheme of serial dilution of a pure culture of V. harveyi in samples of hemolymph or tissues showed an excellent linear correlation, and TaqMan qPCRs have been proven to be powerful and sensitive techniques for V. harveyi detection and quantification in European abalones.…”

Section: Discussionmentioning

confidence: 99%

Vibrio harveyi Adheres to and Penetrates Tissues of the European Abalone Haliotis tuberculata within the First Hours of Contact

Cardinaud

Barbou

Capitaine

et al. 2014

Appl Environ Microbiol

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Vibrio harveyi is a marine bacterial pathogen responsible for episodic epidemics generally associated with massive mortalities in many marine organisms, including the European abalone Haliotis tuberculata. The aim of this study was to identify the portal of entry and the dynamics of infection of V. harveyi in the European abalone. The results indicate that the duration of contact between V. harveyi and the European abalone influences the mortality rate and precocity. Immediately after contact, the epithelial and mucosal area situated between the gills and the hypobranchial gland was colonized by V. harveyi. Real-time PCR analyses and culture quantification of a green fluorescent protein-tagged strain of V. harveyi in abalone tissues revealed a high density of bacteria adhering to and then penetrating the whole gill-hypobranchial gland tissue after 1 h of contact. V. harveyi was also detected in the hemolymph of a significant number of European abalones after 3 h of contact. In conclusion, this article shows that a TaqMan real-time PCR assay is a powerful and useful technique for the detection of a marine pathogen such as V. harveyi in mollusk tissue and for the study of its infection dynamics. Thus, we have revealed that the adhesion and then the penetration of V. harveyi in European abalone organs begin in the first hours of contact. We also hypothesize that the portal of entry of V. harveyi in the European abalone is the area situated between the gills and the hypobranchial gland.T he halophilic Gram-negative bacterium Vibrio harveyi is a common pathogen of many marine vertebrate and invertebrate species (1). V. harveyi is known to induce gastroenteritis, inflammation of the circulatory system or eye, and skin lesions in various species of fishes, crustaceans, and mollusks (2). In the European abalone Haliotis tuberculata, V. harveyi is responsible for the massive mortalities that occur during the summer spawning period both in natural populations and in farmed stocks (3, 4). Vibriosis of the European abalone is characterized by the presence of white spots on the foot and inflammation of the pericardial tissue, resulting in impaired mobility and septicemia (5).Schematically, the bacterial infection process can be summarized to occur in three stages: (i) colonization, adhesion of the pathogen to the host surface, and initial multiplication and then penetration of the pathogen into the body through one or several portals, (ii) invasion of the host organs and/or the circulatory system concurrently with the expression of virulence factors by the pathogen, and (iii) exit of the pathogen and transmission of the disease (6, 7). Invasion and multiplication of V. harveyi inside the circulatory system, the hemolymph, seem to take place after 24 h of contact with the European abalone (5), suggesting that the adhesion and penetration of V. harveyi must occur in the first 24 h of contact. The portal of entry and the precise timing of the earlier stages of V. harveyi infection in the European abalone remain uncharacterized....

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Development of TaqMan real-time PCR assays for monitoring Vibrio harveyi infection and a plasmid harbored by virulent strains in European abalone Haliotis tuberculata aquaculture

Cited by 27 publications

References 40 publications

Quorum sensing inhibitors from Leucetta chagosensis Dendy, 1863

Quorum sensing inhibitors from Leucetta chagosensis Dendy, 1863

Bacterial diseases in marine bivalves

Vibrio harveyi Adheres to and Penetrates Tissues of the European Abalone Haliotis tuberculata within the First Hours of Contact

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