Diagnostic immunohistochemistry for canine cutaneous round cell tumours — retrospective analysis of 60 cases

Paździor-Czapula, Katarzyna; Mikiewicz, Mateusz; Gesek, M.; Zwoliński, Cezary; Otrocka-Domagała, Iwona

doi:10.5603/fhc.a2019.0016

Cited by 8 publications

(22 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…A more precise diagnosis of certain tumors, such as round cell tumors, requires additional histochemical staining methods, so we used toluidine-blue staining to confi rm the diagnosis and to classify the mast cell tumors. In some cases, a more precise classifi cation of round-cell skin tumors requires the use of antibody panels such as MHCII, CD18, Iba1, CD3, CD79a, CD20 and mast cell tryptase [23]. In our study, different forms of mast cell tumors were found in most cases.…”

Section: Discussionmentioning

confidence: 56%

Prevalence and distribution of canine neoplastic and non-neoplastic cutaneous lesions in Serbia: a retrospective study of 2432 Cases (2011 – Mid 2021)

et al. 2021

View full text Add to dashboard Cite

Cutaneous lesions, especially skin tumors in dogs, are among the most common lesions in this animal species. The aim of this study was to identify the most common types of canine cutaneous lesions, to determine the absolute and relative frequency of each type of cutaneous lesion, anatomical locations, mean age, as well as gender and breed distribution. The examination included all samples of cutaneous lesions in dogs obtained by surgical biopsy in veterinary clinics and examined at the Laboratory of the Department of Pathology at the Faculty of Veterinary Medicine, University of Belgrade from the 1st January 2011 to the 1st July 2021. In this period (126 months), a total of 2432 samples of cutaneous lesions were examined, of which 1984 (81.58%) were tumors (1037/1984, 52.27% benign and 947/1984, 47.73% malignant) and 448 (18.42%) non-neoplastic cutaneous lesions. The most commonly found cutaneous tumors were: mast cell tumor (17.34% of all tumors), histiocytoma (9.78%), papilloma (7.91%), lipoma (7.81%), squamous cell carcinoma (7.36%), trichoblastoma (4.44%), hepatoid adenoma (4.39%) and malignant melanoma (4.18%). The most common non-neoplastic cutaneous lesions were: follicular cyst(s) (35.04% of all non-neoplastic lesions), pyogranulomatous chronic dermatitis (23.88%), lymphocytic dermatitis (7.37%), hyperkeratosis (4.24%), and granulomatous dermatitis (3.79%). Our results substantially confirm previously reported data regarding cutaneous neoplastic and nonneoplastic lesions in dogs, and provide updated information on their frequency, animal age, anatomic location and breed distributions.

show abstract

Section: Discussionmentioning

confidence: 56%

Prevalence and distribution of canine neoplastic and non-neoplastic cutaneous lesions in Serbia: a retrospective study of 2432 Cases (2011 – Mid 2021)

et al. 2021

View full text Add to dashboard Cite

show abstract

“…Tissue tryptase expression in canine MCTs has been qualitatively investigated by IHC (Ozaki et al, 2002;Kiupel et al, 2004;Fernandez et al, 2005;Mederle et al, 2010;Pazdzior-Czapula et al, 2019). In a study including 98 dogs, the IHC tryptase pattern…”

Section: Discussionmentioning

confidence: 99%

“…In healthy canine tissues, tryptase has been investigated by ELISA and high levels per mg wet weight were measured in the intestine (Myles et al, 1995). In addition, immunohistochemistry (IHC) of tryptase revealed protein expression in canine cutaneous MCTs (Ozaki et al, 2002;Kiupel et al, 2004;Fernandez et al, 2005;Mederle et al, 2010;Pazdzior-Czapula et al, 2019). Only one study investigated the link between the IHC tryptase staining pattern and the survival time of the dogs with MCT did not find any association based on this qualitative analytical approach (Kiupel et al, 2004).…”

Section: Introductionmentioning

confidence: 99%

Comparison of serum tryptase as a diagnostic oncological marker in canine versus human mast cell neoplasms

Vos

Demeyere

Cock

et al. 2022

Research in Veterinary Science

View full text Add to dashboard Cite

“…In dogs, MCTs are the second most frequently diagnosed malignancy of MCs [42] and belong to the round-cell tumors of the connective tissue [43], which also includes histiocytomas, lymphomas, plasmacytomas, and transmissible venereal tumors. Then, in some cases, immunohistochemical investigation of membrane (lymphocyte or histiocytes markers) or nuclear (MUM-1 (MUltiple Myeloma 1) for plasma cells) antigens are required for diagnosis [44,45].…”

Section: Discussionmentioning

confidence: 99%

A Double Histochemical/Immunohistochemical Staining for the Identification of Canine Mast Cells in Light Microscopy

Migliaccio

Sarli

Silva

et al. 2021

Veterinary Sciences

View full text Add to dashboard Cite

Immunohistochemistry (IHC) is a widely used technique in diagnostic pathology, but the simultaneous analysis of more than one antibody at a time with different chromogens is rather complex, time-consuming, and quite expensive. In order to facilitate the identification of mast cells (MCs) during immunohistochemical analysis of membrane and/or nuclear markers, we propose a new staining method that includes the association of IHC and toluidine blue as a counterstain. To achieve this goal, we tested c-kit, Ki67, and cannabinoid receptor 2 on several cases of cutaneous canine mast cell tumors (MCTs), cutaneous mastocytosis, and atopic dermatitis. The results obtained show how this double staining technique, although limited to non-cytoplasmic markers and of little use in poorly differentiated MCTs in which MC metachromasia is hard to see, can be used during the evaluation of nuclear and/or membranous immunohistochemical markers in all canine cutaneous disorders, especially if characterized by the presence of a low number of MCs. It can help to evaluate those MCTs in which neoplastic MCs must be clearly distinguished from inflammatory cells that can infiltrate the tumor itself, in facilitating the calculation of the Ki67 index. Moreover, it can be used to study the expression of new markers in both animal and human tissues containing MCs and in MC disorders.

show abstract

Diagnostic immunohistochemistry for canine cutaneous round cell tumours — retrospective analysis of 60 cases

Cited by 8 publications

References 0 publications

Prevalence and distribution of canine neoplastic and non-neoplastic cutaneous lesions in Serbia: a retrospective study of 2432 Cases (2011 – Mid 2021)

Prevalence and distribution of canine neoplastic and non-neoplastic cutaneous lesions in Serbia: a retrospective study of 2432 Cases (2011 – Mid 2021)

Comparison of serum tryptase as a diagnostic oncological marker in canine versus human mast cell neoplasms

A Double Histochemical/Immunohistochemical Staining for the Identification of Canine Mast Cells in Light Microscopy

Contact Info

Product

Resources

About