Dietary Selenium Affects Selenoprotein W Gene Expression in the Liver of Chicken

Sun, Beicheng; Wang, Rihua; Li, Jinlong; Jiang, Zhihui; Xu, Shiwen

doi:10.1007/s12011-011-8995-z

Cited by 48 publications

(28 citation statements)

References 36 publications

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“…The data indicated that Se contents in gastrointestinal tract tissues correlated with ingested Se levels. These results confirm previous studies showing that Se affects the expression of SelW [23,32,33].…”

Section: Discussionsupporting

confidence: 92%

“…Previous studies in our lab have shown that the cDNA for chicken SelW can be cloned from Se-supplemented chicken cerebral tissue; we also found that SelW is ubiquitously expressed in diverse tissues of chicken [19,41]. The SelW mRNA distribution in the liver, pancreas, gastrointestinal tissues, and immune organs with different dietary selenium levels have yet to be determined [23,32,33,42]. In the present study, the results of feeding two levels of dietary Se (0.15 and 1.5 mg) for 55 days indicated that SelW gene expression in the chicken gastrointestinal tract was sensitive to dietary Se concentrations and was consistent with these earlier studies.…”

Section: Discussionmentioning

confidence: 74%

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Selenium Regulates Gene Expression of Selenoprotein W in Chicken Gastrointestinal Tract

Gao

Xing

et al. 2011

Biol Trace Elem Res

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Selenoprotein W (SelW) is an existing form of selenium (Se). Se influences the levels of SelW in mammals. However, little is known about the pattern of SelW expression in the gastrointestinal tract tissue of bird. The present paper describes the effects of different dietary levels of Se on the SelW mRNA expression in the gastrointestinal tract tissue of chicken. The expression levels of SelW mRNA and the Se contents in the gastrointestinal tract tissues (glandular stomach, gizzard, duodenum, small intestine, and rectum) were determined on days 15, 25, 35, 45, and 55, respectively. The results showed that the Se contents and the SelW mRNA expression were significantly higher (p < 0.05) in the high-Se group, and the Se contents and SelW mRNA expression in the low-Se group were significantly lower (p < 0.05) than in the controls. The Se contents were the highest in the duodenum and the lowest in the rectum, while the SelW mRNA expression was the highest in the gizzard and the lowest in the rectum. In addition, the SelW mRNA levels in the gastrointestinal tract tissue were found to increase in a time-dependent manner with increasing feeding time. Furthermore, the expression of the SelW mRNA in the gastrointestinal tract tissues of chickens was found to correlate with the dietary Se concentrations, but not with the tissue Se contents.

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Section: Discussionsupporting

confidence: 92%

Section: Discussionmentioning

confidence: 74%

Selenium Regulates Gene Expression of Selenoprotein W in Chicken Gastrointestinal Tract

Gao

Xing

et al. 2011

Biol Trace Elem Res

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“…Se implements its biological function through incorporation into selenoproteins. In addition, Se deficiency caused injuries in several different chicken organs, including the brain (Sheng et al 2014;Xu et al 2013), muscles (Yao et al 2013a, b), the gastrointestinal tract (Gao et al 2012), the spleen (Yu et al 2011) and the liver (Sun et al 2011). The study of Fischer showed that the liver contains a high concentration of Se, which participates in many biological processes that range from cellular antioxidant defense to the protection and repair of DNA to apoptosis (Fischer et al 2006).…”

Section: Introductionmentioning

confidence: 99%

Roles of oxidative stress and endoplasmic reticulum stress in selenium deficiency-induced apoptosis in chicken liver

Yao

et al. 2015

Biometals

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Oxidative stress and endoplasmic reticulum (ER) stress are involved in different types of stress-induced injuries. The aim of the present study was to evaluate the effect of Se deficiency on oxidative stress, ER stress and apoptosis in chicken livers. Chickens (1 day old, n = 180) were randomly divided into two groups: the L group [fed with a Se-deficient (Se 0.033 mg/kg) diet] and the control group [fed with a normal (Se 0.2 mg/kg) diet]. Factor-associated oxidative stress, catalase (CAT) activity, H2O2 production and the inhibition of hydroxyl radicals (·OH) in the chicken liver were determined on days 15, 25, 35, 45, 55 and 65, respectively. In addition, ER stress-related genes (GRP78, GRP94, ATF4, ATF6 and IRE) and apoptosis-related genes (caspase3 and Bcl-2) were examined by fluorescence quantitative PCR or western blot analysis. Apoptosis levels were also measured using ultrastructural observations and the TdT-mediated dUTP nick end labeling assay. The results showed that CAT activity and ·OH inhibition were decreased and that H2O2 production was increased in the low-Se group, which demonstrated that oxidative stress occurred in the chicken liver. The ER stress-related genes (GRP78, GRP94, ATF4, ATF6 and IRE) and the apoptosis-related gene caspase3 were increased (p < 0.05), while Bcl-2 was decreased (p < 0.05) by Se deficiency. In addition, apoptosis and ER lesions were observed by ultrastructural observations of the chicken liver in the low-Se group. The level of apoptosis and the number of apoptotic cells increased with time. These results indicated that the oxidative-ER stress pathway participates in Se deficiency-induced apoptosis in the chicken liver.

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“…SelW expresses ubiquitously in diverse tissues of chicken and particularly high in skeletal muscle tissues [10,33]. Its mRNA distributions in liver, pancreas, gastrointestinal tissues (tongue, esophagus, corp, proventriculus, gizzard, duodenum, small intestine, cecum, and rectum), and immune organs (thymus, bursa of Fabricius, and spleen) with different dietary selenium levels have been determined [21][22][23]34]. Chickens fed the diets containing 1-3 mg/kg sodium selenite have significant increases of SelW mRNA level in the gastrointestinal tract tissues but decreases in the esophagus, crop, proventriculus, gizzard, duodenum, and cecum in chickens fed the diet containing 5 mg/kg sodium selenite [22].…”

Section: Discussionmentioning

confidence: 99%

Selenium Regulates Gene Expression of Selenoprotein W in Chicken Skeletal Muscle System

Ruan

Zhang

et al. 2011

Biol Trace Elem Res

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Selenoprotein W (SelW) is abundantly expressed in skeletal muscles of mammals and necessary for the metabolism of skeletal muscles. However, its expression pattern in skeletal muscle system of birds is still uncovered. Herein, to investigate the distribution of SelW mRNA in chicken skeletal muscle system and its response to different selenium (Se) status, 1-day-old chickens were exposed to various concentrations of Se as sodium selenite in the feed for 35 days. In addition, myoblasts were treated with different concentrations of Se in the medium for 72 h. Then the levels of SelW mRNA in skeletal muscles (wing muscle, pectoral muscle, thigh muscle) and myoblasts were determined on days 1, 15, 25, and 35 and at 0, 24, 48, and 72 h, respectively. The results showed that SelW was detected in all these muscle components and it increased both along with the growth of organism and the differentiation process of myoblasts. The thigh muscle is more responsive to Se intake than the other two skeletal muscle tissues while the optimal Se supplementation for SelW mRNA expression in chicken myoblasts was 10(-7) M. In summary, Se plays important roles in the development of chicken skeletal muscles. To effect optimal SelW gene expression, Se must be provided in the diet and the media in adequate amounts and neither at excessive nor deficient levels.

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Dietary Selenium Affects Selenoprotein W Gene Expression in the Liver of Chicken

Cited by 48 publications

References 36 publications

Selenium Regulates Gene Expression of Selenoprotein W in Chicken Gastrointestinal Tract

Selenium Regulates Gene Expression of Selenoprotein W in Chicken Gastrointestinal Tract

Roles of oxidative stress and endoplasmic reticulum stress in selenium deficiency-induced apoptosis in chicken liver

Selenium Regulates Gene Expression of Selenoprotein W in Chicken Skeletal Muscle System

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