Differences between the effects of EDTA and citrate anticoagulants on platelet count and mean platelet volume

Abstract

Summary Platelet counts on whole blood samples collected into tripotassium salt of EDTA, trisodium citrate (Na3 citr), citrate phosphate dextrose adenine formula 1 (CPDA‐1) and acid citrate dextrose formula A (ACD‐A), all showed a statistically significant drop (P < 0.01) after 1 h standing at room temperature (RT) as compared with the immediate (within 30 min) counts. After 1 h the enumeration became stable in the EDTA samples but the drop continued up to 4–6 h in those samples taken into citrate. The decreases in citrate were significant (18–30%, P < 0.001). The addition of EDTA (1.5 mg/ml) to the citrated samples after the sixth hour count created a significant rise (6–22%, P < 0.01) in the counts between the sixth and the seventh hour. Our observations show that platelet counts in citrated blood samples arc lower than those in EDTA and highlight the necessity to present citrated samples mixed with dried EDTA when characterizationor quality control of blood and blood components is required. Analysis of the mean platelet volume (MPV) showed significantly lower values (6–13%, P < 0.05) in the citrated samples as compared to the same samples in EDTA, and a significant increase (4–6%, P < 0.01) on the addition of EDTA to the citrated samples after the sixth hour analysis.

Changes in the Mean Platelet Volume Levels after Bloodstream Infection Have Prognostic Value

“…For the blood sampling, we used tubes containing EDTA as an anticoagulant, which may have induced platelet swelling. The MPV levels measured in EDTA-preserved samples are reportedly higher than those measured in citrate-preserved samples, and MPV is known to increase on addition of EDTA to citrated samples at six hours after an analysis (27,28). Most samples in our hospital were measured within one hour; thus, the influence of EDTA as an anticoagulant in our analysis is negligible.…”

Comparison of the influence of EDTA-K3 and sodium citrate on haematology analysis in healthy dogs

“…Significantly lower values of HGB in citrate whole blood were the result of lower ability of HGB to oxidate in that anticoagulant. McShine et al (1990) proved that both human platelet count and mean volume (MPV) in citrate blood is significantly lower than in EDTA. Similar observations were made in 1996 by O'Malley et al who used a combination of EDTA and citrate.…”

A phase I trial of intratumoral administration of recombinant oncolytic adenovirus overexpressing HSP70 in advanced solid tumor patients

“…[28][29][30][31] Five of our 27 patients experienced hematological toxicities including one case of grade IV thrombocytopenia at the total dose of 3.0 Â 10 12 VP, a patient whose baseline platelet count was 200 Â 10 9 per l, whereas the mean platelet volume (MPV) was 6.8 fl, much lower than the normal range (9.4-12.5 fl). Although the mean platelet volume measurements can be quite variable, 32 low mean platelet volume implies that inhibition of platelet production occurred before the H103 treatment. This patient recovered after platelet transfusion within 3 months after H103 treatment.…”