Differences between top-down and bottom-up approaches in mineralizing thick, partially demineralized collagen scaffolds

Liu, Yan; Mai, Sui; Li, Nan; Yiu, Cynthia Kar Yung; Mao, Jing; Pashley, David H.; Tay, Franklin R.

doi:10.1016/j.actbio.2010.11.028

Cited by 123 publications

(119 citation statements)

References 49 publications

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“…The development of self-sealing dentine bonding systems with therapeutic ability to create a chemical bond in addition to the micromechanical one, enabling dental restorations to have longer lifetimes, is currently one of the main targets of dental biomaterials research 47) .…”

Section: Development Of Dentine Bonding Systems Comprising Bioactive mentioning

confidence: 99%

Dentine bonding agents comprising calcium-silicates to support proactive dental care: Origins, development and future

Profeta

2014

Dent. Mater. J.

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The origin of ion-releasing dentine bonding agents lies in a change in attitude regarding the qualities demanded of a restorative dental material. The objectives of this paper are to review recent studies on novel hybrid adhesives comprising bioactive fillers based on information from original research papers, reviews, and patent literatures. Literature searches of free text and MeSH terms were performed by using MedLine (PubMed), Web of Science, Scopus, Scielo and the Cochrane Library (6th November, 2013). Reference lists of primary research reports and eligible systematic reviews were cross-checked in an attempt to identify additional studies. Experimental methacrylate-based adhesives, either when incorporating calcium/sodium phosphate-phyllosilicates or calcium silicate cements, demonstrated to promote therapeutic/protective effects on the micro-mechanical and ultramorphological properties of resin bonded-dentine interfaces associated with mineral deposition over time. Further randomized control trials are needed in order to confirm these initial results in vivo.

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Section: Development Of Dentine Bonding Systems Comprising Bioactive mentioning

confidence: 99%

Dentine bonding agents comprising calcium-silicates to support proactive dental care: Origins, development and future

Profeta

2014

Dent. Mater. J.

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“…The biomimetic mineralization through this process is called intrafibrillar mineralization. 8,9,14,[25][26][27] In contrast to the classical crystallization pathways, the nonclassical crystallization route involves mesoscopic transformations of self-assembled, metastable, or amorphous precursor particles into nanoparticulate superstructures. 28,29 Because this process assembles materials from the nanoscopic scale to large sizes, it is also called the bottom-up strategy.…”

Section: Introductionmentioning

confidence: 99%

“…28,29 Because this process assembles materials from the nanoscopic scale to large sizes, it is also called the bottom-up strategy. 8,30 Many previous experiments and studies have shown that biomineralization is a bottom-up process based on the theory of nonclassical crystallization. 8,28,31 The key of the PILP process is to find negatively charged polymers for stabilizing amorphous precursor particles to achieve liquid-liquid phase separation.…”

Section: Introductionmentioning

confidence: 99%

“…8,30 Many previous experiments and studies have shown that biomineralization is a bottom-up process based on the theory of nonclassical crystallization. 8,28,31 The key of the PILP process is to find negatively charged polymers for stabilizing amorphous precursor particles to achieve liquid-liquid phase separation. Many stabilizers have been used in previous experiments, such as polyaspartic acid, polyacrylic acid, poly-l-glutamic acid, etc, which are analogs of dentine matrix protein-1 (DMP-1).…”

Section: Introductionmentioning

confidence: 99%

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Synergistic intrafibrillar/extrafibrillar mineralization of collagen scaffolds based on a biomimetic strategy to promote the regeneration of bone defects

Wang¹,

Manh²,

Wang³

et al. 2016

IJN

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The mineralization of collagen scaffolds can improve their mechanical properties and biocompatibility, thereby providing an appropriate microenvironment for bone regeneration. The primary purpose of the present study is to fabricate a synergistically intra- and extrafibrillar mineralized collagen scaffold, which has many advantages in terms of biocompatibility, biomechanical properties, and further osteogenic potential. In this study, mineralized collagen scaffolds were fabricated using a traditional mineralization method (ie, immersed in simulated body fluid) as a control group and using a biomimetic method based on the polymer-induced liquid precursor process as an experimental group. In the polymer-induced liquid precursor process, a negatively charged polymer, carboxymethyl chitosan (CMC), was used to stabilize amorphous calcium phosphate (ACP) to form nanocomplexes of CMC/ACP. Collagen scaffolds mineralized based on the polymer-induced liquid precursor process were in gel form such that nanocomplexes of CMC/ACP can easily be drawn into the interstices of the collagen fibrils. Scanning electron microscopy and transmission electron microscopy were used to examine the porous micromorphology and synergistic mineralization pattern of the collagen scaffolds. Compared with simulated body fluid, nanocomplexes of CMC/ACP significantly increased the modulus of the collagen scaffolds. The results of in vitro experiments showed that the cell count and differentiated degrees in the experimental group were higher than those in the control group. Histological staining and micro-computed tomography showed that the amount of new bone regenerated in the experimental group was larger than that in the control group. The biomimetic mineralization will assist us in fabricating a novel collagen scaffold for clinical applications.

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“…Nevertheless, even with EDTA agents, it seems that a volume of demineralized and non-resin infiltrated collagen remains at the base (bottom) of the hybrid layer 8 . If effective inhibitors of MMPs are included in resin-dentin bonding interfaces, they may protect the seed crystallite-sparse collagen fibrils of the scaffold from degradation, and they could be remineralized 9 .…”

Section: Introductionmentioning

confidence: 99%

Mechanical loading influences the viscoelastic performance of the resin-carious dentin complex

et al. 2017

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The aim of this study was to evaluate the changes in the mechanical behavior and bonding capability of Zn-doped resin-infiltrated caries-affected dentin interfaces. Dentin surfaces were treated with 37% phosphoric acid (PA) followed by application of a dentin adhesive, Single Bond (SB) (PA+SB) or by 0.5 M ethylenediaminetetraacetic acid (EDTA) followed by SB (EDTA+SB). 10 wt% ZnO microparticles or 2 wt% ZnCl2 were added into SB, resulting in the following groups: PA+SB, PA+SB-ZnO, PA+SB-ZnCl2, EDTA+SB, EDTA+SB-ZnO, EDTA+SB-ZnCl2. Bonded interfaces were stored for 24 h, and tested or submitted to mechanical loading. Microtensile bond strength was assessed. Debonded surfaces were evaluated by scanning electron microscopy and elemental analysis. The hybrid layer, bottom of the hybrid layer, peritubular and intertubular dentin were evaluated using a nanoindenter. The load/displacement responses were used for the nano-dynamic mechanical analysis (nano-DMA III) to estimate complex modulus, tan delta, loss modulus and storage modulus. The modulus mapping was obtained by imposing a quasistatic force setpoint to which a sinusoidal force was superimposed. AFM imaging was performed. Load cycling decreased the tan delta at the PA+SB-ZnCl2 and EDTA+SB-ZnO interfaces. Tan delta was also diminished at peritubular dentin when PA+SB-ZnO was used, hindering the dissipation of energy throughout these structures. Tan delta increased at the interface after using EDTA+SB-ZnCl2, lowering the energy for recoil or failure. After load cycling, loss moduli at the interface decreased when using ZnCl2 as doping agent, increasing the risk of fracture; but when using ZnO loss moduli was dissimilarly affected if dentin was EDTA-treated. The border between intertubular and peritubular dentin attained the highest discrepancy in values of viscoelastic properties, meaning a risk for cracking and breakdown of the resin-dentin interface. PA used on dentin provoked differences in 4 complex and storage modulus values at the intertubular and peritubular structures, and these differences were higher than when EDTA was employed. In these cases, the longterm performance of the restorative interface will be impared.

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Differences between top-down and bottom-up approaches in mineralizing thick, partially demineralized collagen scaffolds

Cited by 123 publications

References 49 publications

Dentine bonding agents comprising calcium-silicates to support proactive dental care: Origins, development and future

Dentine bonding agents comprising calcium-silicates to support proactive dental care: Origins, development and future

Synergistic intrafibrillar/extrafibrillar mineralization of collagen scaffolds based on a biomimetic strategy to promote the regeneration of bone defects

Mechanical loading influences the viscoelastic performance of the resin-carious dentin complex

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