Differences in cell migration of cultured pituitary cells from infantile and adult rats: participation of the extracellular matrix and epidermal growth factor

González, B Puga; Solano-Agama, Maria del Carmen; Pliego, Margarita González del; Mendoza-Garrido, M. Eugenia

doi:10.1016/j.ijdevneu.2004.02.003

Cited by 7 publications

(8 citation statements)

References 43 publications

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“…During early postnatal development the pituitary undergoes architectural changes that involve migration and ECM remodeling. This process is regulated by epidermal growth factor (26). Furthermore, it has been shown that laminin controls the release of prolactin and gonadotropins in the normal pituitary (27).…”

Section: The Pituitary Glandmentioning

confidence: 99%

Regulation of pituitary hormones and cell proliferation by components of the extracellular matrix

Páez-Pereda

Kuchenbauer

Arzt

et al. 2005

Braz J Med Biol Res

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The extracellular matrix is a three-dimensional network of proteins, glycosaminoglycans and other macromolecules. It has a structural support function as well as a role in cell adhesion, migration, proliferation, differentiation, and survival. The extracellular matrix conveys signals through membrane receptors called integrins and plays an important role in pituitary physiology and tumorigenesis. There is a differential expression of extracellular matrix components and integrins during the pituitary development in the embryo and during tumorigenesis in the adult. Different extracellular matrix components regulate adrenocorticotropin at the level of the proopiomelanocortin gene transcription. The extracellular matrix also controls the proliferation of adrenocorticotropin-secreting tumor cells. On the other hand, laminin regulates the production of prolactin. Laminin has a dynamic pattern of expression during prolactinoma development with lower levels in the early pituitary hyperplasia and a strong reduction in fully grown prolactinomas. Therefore, the expression of extracellular matrix components plays a role in pituitary tumorigenesis. On the other hand, the remodeling of the extracellular matrix affects pituitary cell proliferation. Matrix metalloproteinase activity is very high in all types of human pituitary adenomas. Matrix metalloproteinase secreted by pituitary cells can release growth factors from the extracellular matrix that, in turn, control pituitary cell proliferation and hormone secretion. In summary, the differential expression of extracellular matrix components, integrins and matrix metalloproteinase contributes to the control of pituitary hormone production and cell proliferation during tumorigenesis.

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Section: The Pituitary Glandmentioning

confidence: 99%

Regulation of pituitary hormones and cell proliferation by components of the extracellular matrix

Páez-Pereda

Kuchenbauer

Arzt

et al. 2005

Braz J Med Biol Res

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“…Briefly, ECM remodeling is an actively synthesized or degraded process, and the presence of growth factors probably induces the variance of ECM components, or stimulates the accumulation of ECM or influences cell migration rate in ECM resulting in the change of ECM structure and stability, sequentially regulate ECM remodeling and even tissue remodeling. González et al [27] examined anterior pituitary cell migration over different type collagen of cultured pituitary cells from infantile rats and adult rats, it is indicated that infantile cells increased their migration rate with EGF stimulation, while adult cells showed a decrease in migration when the growth factor was in the medium. During migration, pituitary cells associated and arranged in clusters.…”

Section: Effects Growth Factors Exerting On Ecmmentioning

confidence: 99%

The multiple interactions between growth factors and microenvironment in vivo

Yang

Zhu

et al. 2006

Sci. Bull.

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“…We have shown that cultured infantile pituitary cells migrate preferentially over collagen type I/III, and the epidermal growth factor (EGF) stimulates this behaviour. Moreover, EGF stimulation induces an increase in cell association (González et al, 2004) and cell spreading (Toral et al, 2003).The aim of the present study was to investigate more about the migration and association behaviour of SC and FS using a vertical diffusion chamber. Aliquots of 3 × 10 5 rat infantile pituitary cells were placed in the upper compartment without or with EGF (50 ng/ml) in a defined culture medium.…”

mentioning

confidence: 97%

mentioning

confidence: 99%

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[P97]: Real‐time imaging of migrating cortical interneurons

Britto

Johnston

Tan

2006

Intl J of Devlp Neuroscience

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Poster abstracts / Int. J. Devl Neuroscience 24 (2006) 495-603 537 cells (FS) (Inoue et al., 1999). During early postnatal development, rat anterior pituitary cells re-accommodate in the gland tissue, implicating intense migration activity. We have shown that cultured infantile pituitary cells migrate preferentially over collagen type I/III, and the epidermal growth factor (EGF) stimulates this behaviour. Moreover, EGF stimulation induces an increase in cell association (González et al., 2004) and cell spreading (Toral et al., 2003).The aim of the present study was to investigate more about the migration and association behaviour of SC and FS using a vertical diffusion chamber. Aliquots of 3 × 10 5 rat infantile pituitary cells were placed in the upper compartment without or with EGF (50 ng/ml) in a defined culture medium. Cells were allow migrating to the lower compartment through the 12 m pore size and settled on a cover glass coated with collagen I/III. After 48 h, the migrated cells were fixed and stained with toluidine blue or assay for fluorescent-immunocytochemistry for S-100␤, identifying FS, chromogranin A, identifying SC, nucleus with To-Pro-3iodide and F-actin with TRICT-phalloidin. Cells were visualized with a light microscope for cell number measurement, or with a confocal microscope.When cells were stimulated with EGF an increase in cell migration and cell association were observed. In FS, S-100␤ was observed like fibres crossing the cell body and in membrane outgrowth processes. These membrane extensions were directed to other FS. When both cell types were forming clusters, S-100␤ fibres were observed like involving the cluster. Also, a thin S-100␤ band was seen in the boundary between FS and SC.These data suggest a participation of S-100␤ in the mechanism of cell interaction between FSs and with SCs in the cluster formation.

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Differences in cell migration of cultured pituitary cells from infantile and adult rats: participation of the extracellular matrix and epidermal growth factor

Cited by 7 publications

References 43 publications

Regulation of pituitary hormones and cell proliferation by components of the extracellular matrix

Regulation of pituitary hormones and cell proliferation by components of the extracellular matrix

The multiple interactions between growth factors and microenvironment in vivo

[P97]: Real‐time imaging of migrating cortical interneurons

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