Different blood collection methods from rats: A review

Kumar, M Kiran; Dandapat, Sukumar; Sinha, Manoranjan; Kumar, Amar Nagesh; Raipat, Bharti Singh

doi:10.12680/balneo.2017.141

Cited by 37 publications

(34 citation statements)

References 5 publications

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“…Blood samples in rat groups I-III, which were terminated three hours after birth, were taken via jugular vein, was previously given an anesthetic cream ( Parasuraman et al, 2010 ). Meanwhile, blood samples of all neonatal rat groups in the following times were taken by cardiac puncture, and the blood samples were collected into Eppendorf tubes containing EDTA ( Kumar et al, 2017 ).…”

Section: Methodsmentioning

confidence: 99%

Immunomodulation of tahneeq method in IL-12 and CD8+ T-Lymphocyte, an in-vivo study in neonatal rats

Susilorini

Suradi

Indarto

et al. 2020

Saudi Journal of Biological Sciences

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Stimulation of the neonatal immune system is quite important for the proliferation and differentiation of antigen-presenting cells (APCs) and T cells. Tahneeq is a traditional method to manually rub the palatal mucosa of newborn babies with premasticated Ajwa palm dates. The present study was to investigate the tahneeq effects on IL-12 expression of dendritic cells (DCs) and blood T lymphocytes expressing CD8 + in neonatal Wistar rats. The number of 90 healthy neonatal Wistar rats have randomly divided into three groups: control group received breastmilk only, treatment group (T1) receiving breast milk + mild-scratched intensity of tahneeq, and T2 group received breastmilk + strong-scratched intensity of tahneeq on the palatal and gingival mucosa immediately after birth. Seven neonatal Wistar rats in all groups were then sacrificed in three hours after birth and days 1, 5, 7, 13, and 30 treatment. IL-12 expression in the palatal and gingival mucosa was determined using immunohistochemical staining, and blood CD8 + T-lymphocytes were quantified using a flow cytometer. One way ANOVA was used to analyze the percentage of IL-12 and CD8 + T-lymphocytes among neonatal Wistar rat groups. The T1 and T2 newborn rat groups had significantly higher IL-12 expression than the control group (p<0.001). The increased IL-12 expression in T2 groups significantly increased (p<0.001) compared to the IL-12 expression in the T1 and control groups. The percentage of CD8 + T lymphocytes in all neonatal rat groups increased on three hours after birth and day 30 treatment but remained constant on days 5 and 7 treatment and decreased on day 13 treatment. At 5, 13, and 30 th days treatment, the percentage of CD8 + T lymphocytes in T1 and T2 neonatal rat groups was significantly higher (p<0.05) than that in the control group. In conclusion, the impact on systemic CD8 + T cells did not influence by the depth of the scratch. Both mild and strong tahneeq increased the systemic CD8 + T-lymphocytes in neonatal Wistar rats. The roles of anti-inflammatory cytokines and Treg cells should be further investigated to unravel those different results for the development of mucosal immunity in neonates.

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Section: Methodsmentioning

confidence: 99%

Immunomodulation of tahneeq method in IL-12 and CD8+ T-Lymphocyte, an in-vivo study in neonatal rats

Susilorini

Suradi

Indarto

et al. 2020

Saudi Journal of Biological Sciences

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“…The tail vein was used to draw 1.5 ~ 2 ml of whole blood using a 26 G needle from animals. The blood was stored in a sterile blood collection tube with citrate-phosphate-dextrose-adenine (CPDA-1) [20]. Platelet-free plasma was separated by centrifugation at 3000 G for 20 min followed by removal of the platelet-rich plasma region.…”

Section: Methodsmentioning

confidence: 99%

Optofluidic laser speckle image decorrelation analysis for the assessment of red blood cell storage

Jeon¹,

Qureshi²,

Lee³

et al. 2019

PLoS ONE

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Red blood cells (RBCs) undergo irreversible biochemical and morphological changes during storage, contributing to the hemorheological changes of stored RBCs, which causes deterioration of microvascular perfusion in vivo. In this study, a home-built optofluidic system for laser speckle imaging of flowing stored RBCs through a transparent microfluidic channel was employed. The speckle decorrelation time (SDT) provides a quantitative measure of RBC changes, including aggregation in the microchannel. The SDT and relative light transmission intensity of the stored RBCs were monitored for 42 days. In addition, correlations between the decorrelation time, RBC flow speed through the channel, and relative light transmission intensity were obtained. The SDT of stored RBCs increased as the storage duration increased. The SDTs of the RBCs stored for 21 days did not significantly change. However, for the RBCs stored for over 35 days, the SDT increased significantly from 1.26 ± 0.27 ms to 6.12 ± 1.98 ms. In addition, we measured the relative light transmission intensity and RBC flow speed. As the RBC storage time increased, the relative light transmission intensity increased, whereas the RBC flow speed decreased in the microchannel. The optofluidic laser speckle image decorrelation time provides a quantitative measure of assessing the RBC condition during storage. Laser speckle image decorrelation analysis may serve as a convenient assay to monitor the property changes of stored RBCs.

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“…The rest of the heparinized sample was transferred to a microcentrifuge tube and run for 20 min at 5000 rpm (4 • C) to obtain serum. The serum was shifted to a clean microcentrifuge tube and incubated at −80 • C ( Kumar et al, 2017).…”

Section: Blood Collectionmentioning

confidence: 99%

Effect of Katakakhadiradi kashayam on liver metabolism in streptozotocin and nicotinamide induced diabetic rats

Subbiah¹,

Kavimani²,

Ramadoss³

et al. 2021

ijrps

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Plants have the ability to impart therapeutic action on chronic diseases such as diabetes and its comorbidities. The current study was done to find the effectiveness of Katakakhadiradi kashayam on liver metabolism in streptozotocin and nicotinamide administered diabetic rats. Diabetic rats were treated with katakakhadiradi kashayam for 28 days and compared with glibenclamide. The antidiabetic action of Katakakhadiradi kashayam was measured by the serum levels of urea, bilirubin, SGOT and SGPT activity. In the current study, the level of urea, SGPT and SGOT activity was significantly higher and reduced total bilirubin observed in diabetic rats. The protection given by Katakakhadiradi kashayam against diabetic complication was confirmed by a histology study. Most of the plants present in Kadakakhadiradi kashayam are effective in regulating the enzymes in diabetes mellitus. The obtained data imply the antidiabetic effects of katakakhadiradi kashayam, which is practically a safe herbal formulation and can be used as a good alternative for managing DM.

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Different blood collection methods from rats: A review

Cited by 37 publications

References 5 publications

Immunomodulation of tahneeq method in IL-12 and CD8+ T-Lymphocyte, an in-vivo study in neonatal rats

Immunomodulation of tahneeq method in IL-12 and CD8+ T-Lymphocyte, an in-vivo study in neonatal rats

Optofluidic laser speckle image decorrelation analysis for the assessment of red blood cell storage

Effect of Katakakhadiradi kashayam on liver metabolism in streptozotocin and nicotinamide induced diabetic rats

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