Differential alkaline phosphatase responses of rat and human bone marrow derived mesenchymal stem cells to 45S5 bioactive glass

Reilly, Gwendolen C.; Radin, S.; Chen, Andrew T.; Ducheyne, Paul

doi:10.1016/j.biomaterials.2007.05.038

Cited by 146 publications

(137 citation statements)

References 40 publications

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“…ALP and OC are well-known bone markers. 17,19,28,50 Runx2 (runtrelated transcription factor 2) regulates skeletal development, and it is a crucial transcription gene within the osteogenic phenotype. 65 At 8 days, ALP, OC, collagen I, and Runx2 were all higher for hUCMSCs on CPC with 8% collagen than that on CPC without collagen.…”

Section: Thein-han and Xumentioning

confidence: 99%

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Collagen-Calcium Phosphate Cement Scaffolds Seeded with Umbilical Cord Stem Cells for Bone Tissue Engineering

Thein-Han

2011

Tissue Engineering Part A

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Human umbilical cord mesenchymal stem cells (hUCMSCs) avoid the invasive procedure required to harvest bone marrow MSCs. The addition of collagen fibers into self-setting calcium phosphate cement (CPC) may increase the scaffold strength, and enhance cell attachment and differentiation. The objectives of this study were to develop a novel class of collagen-CPC composite scaffolds, and to investigate hUCMSC attachment, proliferation, and osteogenic differentiation on collagen-CPC scaffolds for the first time. Collagen fibers in CPC improved the load-bearing capability. Flow cytometry showed that the hUCMSCs expressed cell surface markers characteristic of MSCs, and were negative for hematopoietic and endothelial cell markers. hUCMSCs proliferated rapidly in all CPC composite scaffolds, with cell number increasing by sevenfold in 8 days. Cellular function was enhanced with collagen fibers in CPC scaffolds. Cell density increased from (645 -60) cells/mm 2 on CPC with 0% collagen, to (1056 -65) cells/mm 2 on CPC with 8% collagen ( p < 0.05). The actin stress fibers inside the hUCMSCs were stained, and the fluorescence intensity was doubled when the collagen in CPC was increased by 0% to 8%. RT-PCR showed that hUCMSCs on CPC with collagen had higher osteogenic expression than those on CPC without collagen. Alizarin Red S staining revealed a great increase in mineralization by hUCMSCs on CPC with collagen than that without collagen. In conclusion, hUCMSCs showed excellent proliferation, differentiation, and synthesis of bone minerals in collagen-CPC composite scaffolds for the first time. The novel hUCMSC-seeded collagen-CPC construct with superior cell function and load-bearing capability is promising to enhance bone regeneration in a wide range of orthopedic and craniofacial applications.

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Section: Thein-han and Xumentioning

confidence: 99%

“…CaP bioceramics such as hydroxyapatite (HA) are important for bone repair because of their similarity to the bone minerals. [25][26][27][28][29] Bone is comprised of collagen (mainly type I) and nano-HA crystals. Hence, synthetic collagen-HA composites are promising in mimicking and replacing bone.…”

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confidence: 99%

Collagen-Calcium Phosphate Cement Scaffolds Seeded with Umbilical Cord Stem Cells for Bone Tissue Engineering

Thein-Han

2011

Tissue Engineering Part A

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“…[12][13][14][15][16] Studies have shown promise in stem cell delivery using scaffolds for regenerative medicine applications. [17][18][19][20] Calcium phosphate scaffolds mimic bone minerals and can facilitate cell attachment and function. 14,[21][22][23] They are bioactive and can bond to bone to form a functional interface.…”

Section: Introductionmentioning

confidence: 99%

“…14,[21][22][23] They are bioactive and can bond to bone to form a functional interface. 8,18,24,25 Calcium phosphate cements (CPCs) have good biocompatibility and osteoconductivity. [26][27][28][29][30][31][32] CPCs have an advantage over prefabricated bioceramics, which require the surgeon to carve the surgical site and machine the graft to fit the bone cavity.…”

Section: Introductionmentioning

confidence: 99%

Gas-Foaming Calcium Phosphate Cement Scaffold Encapsulating Human Umbilical Cord Stem Cells

Chen

Zhou

Tang

et al. 2012

Tissue Engineering Part A

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Tissue engineering approaches are promising to meet the increasing need for bone regeneration. Calcium phosphate cement (CPC) can be injected and self-set to form a scaffold with excellent osteoconductivity. The objectives of this study were to develop a macroporous CPC-chitosan-fiber construct containing alginate-fibrin microbeads encapsulating human umbilical cord mesenchymal stem cells (hUCMSCs) and to investigate hUCMSC release from the degrading microbeads and proliferation inside the porous CPC construct. The hUCMSC-encapsulated microbeads were completely wrapped inside the CPC paste, with the gas-foaming porogen creating macropores in CPC to provide for access to culture media. Increasing the porogen content in CPC significantly increased the cell viability, from 49% of live cells in CPC with 0% porogen to 86% of live cells in CPC with 15% porogen. The alginate-fibrin microbeads started to degrade and release the cells inside CPC at 7 days. The released cells started to proliferate inside the macroporous CPC construct. The live cell number inside CPC increased from 270 cells/mm 2 at 1 day to 350 cells/mm 2 at 21 days. The pore volume fraction of CPC increased from 46.8% to 78.4% using the gas-foaming method, with macropore sizes of approximately 100 to 400 mm. The strength of the CPC-chitosan-fiber scaffold at 15% porogen was 3.8 MPa, which approximated the reported 3.5 MPa for cancellous bone. In conclusion, a novel gas-foaming macroporous CPC construct containing degradable alginate-fibrin microbeads was developed that encapsulated hUCMSCs. The cells had good viability while wrapped inside the porous CPC construct. The degradable microbeads in CPC quickly released the cells, which proliferated over time inside the porous CPC. Self-setting, strong CPC with alginate-fibrin microbeads for stem cell delivery is promising for bone tissue engineering applications.

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“…They include calcium phosphate ceramics [1][2][3][4], hydroxyapatite (HA) ceramic [5][6], bioactive glass (BG) [7][8][9][10] and calcium phosphate cements (CPC) [11][12][13][14][15]. Some of these materials have been used in the clinic with excellent results [16][17][18][19].…”

Section: Introductionmentioning

confidence: 99%

In vitrosurface reaction layer formation and dissolution of calcium phosphate cement–bioactive glass composites

2008

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Composites of hydrated calcium phosphate cement (CPC) and bioactive glass (BG) containing Si were immersed in vitro to study the effect of chemical composition on surface reaction layer formation and dissolution/precipitation behavior. The solutions used were 0.05M tris hydroxymethyl aminomethane/HCl (tris buffer), tris buffer supplemented with plasma electrolyte (TE) with pH 7.4 at 37°C, and this solution complemented with 10% newborn bovine serum (TES). The post-immersion solutions were analyzed for changes in Ca, PO 4 and Si concentrations. The reacted surfaces were analyzed using Fourier transform infrared (FTIR), and scanning electron microscopy (SEM) with energy dispersive X-ray analysis (EDX). The sample weight variations after immersion were also determined.The results showed that the composition of the bioactive composite CPCs greatly affected their behavior in solution and the formation of apatite bioactive surface reaction layers. After immersion in TE solution, Ca ions were taken up by all samples during the entire immersion duration. Initially, the P ion concentration increased sharply, and then decreased. This reaction pattern reveals the formation of an amorphous calcium phosphate layer on the surface of these composite calcium phosphate cements. FTIR revealed that the layer was, in fact, poorly crystallized Ca-deficient carbonate apatite. The thickness of the layer was 12-14 μm and was composed of rod-like apatite with directional arrangement. For immersion in TES solution, the Ca and Si ion concentrations showed a similar behavior as that in TE, but the release rate of Si ion was higher. FTIR revealed that after TES immersion, not only did the typical, poorly crystallized, Ca-deficient carbonated apatite form, as it did in TE, but that the serum proteins co-adsorbed on the surface and thereby affected the surface reaction layer formation. A thinner apatite layer was formed and was composed of a micro-porous layer comprising rounded particles in a glue-like appearing matrix. The addition of BG to the calcium phosphate cements to create composite calcium phosphate cements obviously is at the basis of this altered behavior of the cements. All data combined are useful for the design and optimization of degradable implant materials for use in bone tissue repair and regeneration procedures. The solutions used were 0.05M tris hydroxymethyl aminomethane/HCl (tris buffer), tris buffer supplemented with plasma electrolyte (TE) with pH 7.4 at 37°C, and this solution complemented with 10% newborn bovine serum (TES). The post-immersion solutions were analyzed for changes in Ca, PO4 and Si concentrations. The reacted surfaces were analyzed using Fourier transform infrared (FTIR), and scanning electron microscopy (SEM) with energy dispersive X-ray analysis (EDX). The sample weight variations after immersion were also determined.The results showed that the composition of the bioactive composite CPCs greatly affected their behavior in solution and the formation of apatite bioactive surface reaction layer...

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Differential alkaline phosphatase responses of rat and human bone marrow derived mesenchymal stem cells to 45S5 bioactive glass

Cited by 146 publications

References 40 publications

Collagen-Calcium Phosphate Cement Scaffolds Seeded with Umbilical Cord Stem Cells for Bone Tissue Engineering

Collagen-Calcium Phosphate Cement Scaffolds Seeded with Umbilical Cord Stem Cells for Bone Tissue Engineering

Gas-Foaming Calcium Phosphate Cement Scaffold Encapsulating Human Umbilical Cord Stem Cells

In vitrosurface reaction layer formation and dissolution of calcium phosphate cement–bioactive glass composites

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