Differential gene expression in banana roots in response to Fusarium wilt

Wang, Yuguang; Xia, Qiyu; Wang, Guihua; He, Zuyong; Lu, Xuehua; Sun, Jianbo; Zhang, Xin

doi:10.1080/07060661.2017.1342693

Cited by 19 publications

(16 citation statements)

References 34 publications

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“…cubense (Foc) ( Wang et al, 2015 ). Especially, the Foc Tropical Race 4 (Foc TR4) is regarded as the most disastrous race and attacks almost all banana cultivars, thereby causing extensive destruction of banana orchards ( Shen et al, 2015 ; Wang et al, 2017 ). Until now, there is no an effective physical or chemical strategy to prevent the spread of fungal disease.…”

Section: Introductionmentioning

confidence: 99%

A Newly Isolated Streptomyces sp. YYS-7 With a Broad-Spectrum Antifungal Activity Improves the Banana Plant Resistance to Fusarium oxysporum f. sp. cubense Tropical Race 4

et al. 2020

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Fusarium wilt of banana caused by Fusarium oxysporum f. sp. cubense (Foc) is one of the most destructive diseases, severely limiting the development of banana industry. Especially, Foc tropical race 4 (Foc TR4) can infect and destroy almost all banana cultivars. Until now, there is still a lack of an effective method for controlling fusarium wilt. A biocontrol strategy using Actinobacteria is considered as a promising method for management of disease and pest. In this study, 229 Actinobacteria were isolated from rhizosphere soil samples of a primitive ecological mountain. An actinobacterium strain marked with YYS-7 exhibited a high antifungal activity against Foc TR4. Combining the physiological and biochemical characteristics as well as alignment of the 16S rRNA sequence, the strain YYS-7 was assigned to Streptomyces sp. The crude extracts of Streptomyces sp. YYS-7 obviously inhibited the mycelial growth of Foc TR4. The cell integrity and ultrastructure were seriously destroyed. In addition, Streptomyces sp. YYS-7 and crude extracts also showed a broad-spectrum antifungal activity against the selected seven phytopathogenic fungi. A gas chromatography-mass spectrometry (GC-MS) was used to predict the antifungal metabolites. A total of eleven different compounds were identified, including phenolic compounds, hydrocarbons, esters and acids. In the pot experiment, the crude extracts can significantly improve the banana plant's resistance to Foc TR4. Hence, Streptomyces sp. YYS-7 will be a potential biocontrol agent for the biofertilizer exploitation and the discovery of new bioactive substances.

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Section: Introductionmentioning

confidence: 99%

A Newly Isolated Streptomyces sp. YYS-7 With a Broad-Spectrum Antifungal Activity Improves the Banana Plant Resistance to Fusarium oxysporum f. sp. cubense Tropical Race 4

et al. 2020

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“…RNA sequencing (RNA-Seq) analysis is a powerful approach to study transcriptomes, and has led the way to identify BTH-responsive signaling pathways tightly linked to Fusarium wilt that are highly valued in breeding. Importantly, previous studies mainly focused on studying the molecular mechanism of susceptible banana infected with Foc 4 [ 11 , 36 – 39 ], a global analysis of the transcriptome responses associated with resistance inducer has not yet been performed. Therefore, to determine the effects of BTH on plant metabolism, we analyzed the transcriptome of Cavendish banana leaves and roots treated with BTH.…”

Section: Introductionmentioning

confidence: 99%

Genome-wide transcriptome analysis and identification of benzothiadiazole-induced genes and pathways potentially associated with defense response in banana

Cheng

et al. 2018

BMC Genomics

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BackgroundBananas (Musa spp.) are the most important fruit crops worldwide due to their high nutrition value. Fusarium wilt of banana, caused by fungal pathogen Fusarium oxysporum f. sp. cubense tropical race 4 (Foc 4), is considered as the most destructive disease in the world and results in extensive damage leading to productivity loss. The widespread use of plant resistance inducers (PRIs), such as benzothiadiazole (BTH), is a novel strategy to stimulate defense responses in banana plants to protect against pathogens infection. The recent focus on the crop defense against fungal infections has led to a renewed interest on understanding the molecular mechanisms of specific PRIs-mediated resistance. This transcriptome study aimed to identify genes that are associated with BTH-induced resistance. Patterns of gene expression in the leaves and roots of BTH-sprayed banana plants were studied using RNA-Seq.ResultsIn this study, 18 RNA-Seq libraries from BTH-sprayed and untreated leaves and roots of the Cavendish plants, the most widely grown banana cultivar, were used for studying the transcriptional basis of BTH-related resistance. Comparative analyses have revealed that 6689 and 3624 differentially expressed genes were identified in leaves and roots, respectively, as compared to the control. Approximately 80% of these genes were differentially expressed in a tissue-specific manner. Further analysis showed that signaling perception and transduction, transcription factors, disease resistant proteins, plant hormones and cell wall organization-related genes were stimulated by BTH treatment, especially in roots. Interestingly, the ethylene and auxin biosynthesis and response genes were found to be up-regulated in leaves and roots, respectively, suggesting a choice among BTH-responsive phytohormone regulation.ConclusionsOur data suggests a role for BTH in enhancing banana plant defense responses to Foc 4 infection, and demonstrates that BTH selectively affect biological processes associated with plant defenses. The genes identified in the study could be further studied and exploited to develop Foc 4-resistant banana varieties.Electronic supplementary materialThe online version of this article (10.1186/s12864-018-4830-7) contains supplementary material, which is available to authorized users.

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“…malaccensis , a progenitor of the current cultivated banana, was the first Musa genome sequenced 5 , followed by a draft release of the genome of another banana progenitor, M. balbisiana 6 , and a close relative, M. itinerans 7 . In addition, a number of gene expression profiles of different banana cultivars, tissues, and conditions have also been investigated 8–14 . Despite the success of banana genomic and transcriptomic sequencing projects, reverse genetic approaches for identifying and characterizing gene functions in banana remain a challenge.…”

Section: Introductionmentioning

confidence: 99%

Cucumber mosaic virus-induced gene silencing in banana

Tzean

Lee

Jan

et al. 2019

Sci Rep

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Banana ( Musa spp.) is one of the world’s most important staple and cash crops. Despite accumulating genetic and transcriptomic data, low transformation efficiency in agronomically important Musa spp. render translational researches in banana difficult by using conventional knockout approaches. To develop tools for translational research in bananas, we developed a virus induced-gene silencing (VIGS) system based on a banana-infecting cucumber mosaic virus (CMV) isolate, CMV 20. CMV 20 genomic RNA 1, 2, and 3, were separately cloned in Agrobacterium pJL89 binary vectors, and a cloning site was introduced on RNA 2 immediately after the 2a open reading frame to insert the gene targeted for silencing. An efficient Agrobacterium inoculation method was developed for banana, which enabled the CMV 20 VIGS vector infection rate to reach 95% in our experiments. CMV 20-based silencing of Musa acuminata cv. Cavendish (AAA group) glutamate 1-semialdehyde aminotransferase ( MaGSA ) produced a typical chlorotic phenotype and silencing of M. acuminata phytoene desaturase ( MaPDS ) produced a photobleachnig phenotype. We show this approach efficiently reduced GSA and PDS transcripts to 10% and 18% of the control, respectively. The high infection rate and extended silencing of this VIGS system will provide an invaluable tool to accelerate functional genomic studies in banana.

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Differential gene expression in banana roots in response to Fusarium wilt

Cited by 19 publications

References 34 publications

A Newly Isolated Streptomyces sp. YYS-7 With a Broad-Spectrum Antifungal Activity Improves the Banana Plant Resistance to Fusarium oxysporum f. sp. cubense Tropical Race 4

A Newly Isolated Streptomyces sp. YYS-7 With a Broad-Spectrum Antifungal Activity Improves the Banana Plant Resistance to Fusarium oxysporum f. sp. cubense Tropical Race 4

Genome-wide transcriptome analysis and identification of benzothiadiazole-induced genes and pathways potentially associated with defense response in banana

Cucumber mosaic virus-induced gene silencing in banana

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