Differential protein expression in two bivalve species; Mytilus galloprovincialis and Corbicula fluminea; exposed to Cylindrospermopsis raciborskii cells

Puerto, María; Campos, Alexandre; Prieto, Ana I.; Cameán, Ana M.; Almeida, André M.; Coelho, Ana Varela; Vasconcelos, Vı́tor

doi:10.1016/j.aquatox.2010.09.009

Cited by 67 publications

(57 citation statements)

References 56 publications

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“…The similar alteration of actin and tubulin has been also observed in response to cellular stress and apoptosis in M. galloprovincialis exposed to Cylindrospermopsis raciborskii cells (Puerto et al, 2011). Therefore, the alterations of actin and tubulin suggested the cellular stress and apoptosis in TBBPA-treated male mussel samples, which was also evidenced by TUNEL assay.…”

Section: Cytoskeletonmentioning

confidence: 52%

iTRAQ-based quantitative proteomic analyses on the gender-specific responses in mussel Mytilus galloprovincialis to tetrabromobisphenol A

Wei

et al. 2014

Aquatic Toxicology

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a b s t r a c tTetrabromobisphenol A (TBBPA) accounts for the largest production of brominated flame-retardants (BFRs) along the Laizhou Bay in China and is the most widely used BFR in industrial products. It can induce diverse toxicities including hepatotoxicity, nephrotoxicity, neurotoxicity and endocrine disrupting effects in mammalian and fish models. In this work, we applied iTRAQ-based proteomics to investigate the gender-specific responses in mussel Mytilus galloprovincialis to TBBPA. Thirty-one proteins were differentially expressed in hepatopancreas between male and female mussels, which clearly indicated the biological differences between male and female mussels at the protein level. After exposure of TBBPA (18.4 nmol/L) for one month, a total of 60 proteins were differentially expressed in response to the TBBPA treatment in mussel hepatopancreas, among which 33 and 29 proteins were significantly altered in TBBPA-treated male and female mussel samples, respectively. Only two of the 60 proteins were commonly altered in both male and female mussel samples exposed to TBBPA. Based on KEGG analysis, these differentially expressed proteins of TBBPA-induced effects were assigned to several groups, including cytoskeleton, reproduction and development, metabolism, signal transduction, gene expression, stress response and apoptosis. Overall, results indicated that TBBPA exposure could induce apoptosis, oxidative and immune stresses and disruption in energy, protein and lipid metabolisms in both male and female mussels with different mechanisms. This work suggested that the gender differences should be considered in ecotoxicoproteomics.

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Section: Cytoskeletonmentioning

confidence: 52%

iTRAQ-based quantitative proteomic analyses on the gender-specific responses in mussel Mytilus galloprovincialis to tetrabromobisphenol A

Wei

et al. 2014

Aquatic Toxicology

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“…In the Atlantic cod (Gadus morhua) exposed to 2,2 ,4,4 ,5,5 -hexachlorobiphenyl (PCB 153), Berg et al (2011) found a similar alteration in the ubiquitinproteasome system. The alteration of tubulin alpha-8 chain was the protein marker of cellular injury (Puerto et al, 2011). However, no significant proteomic differences were found between the two experimental concentrations of BDE 47 in male mussel gills.…”

Section: Effects Of Bde 47 On the Proteome Of Mussel Gillsmentioning

confidence: 89%

Proteomic and metabolomic analysis reveal gender-specific responses of mussel Mytilus galloprovincialis to 2,2′,4,4′-tetrabromodiphenyl ether (BDE 47)

Wei

et al. 2013

Aquatic Toxicology

101

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“…Actin is an abundant cytoskeletal protein that is a component of microfilaments in cells (Puerto et al, 2011). Both F-actin-capping protein and twinfilin are actin-binding proteins that are involved in the regulation of actin dynamics (Hartmann et al, 1989;Matzavinos and Othmer, 2007).…”

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confidence: 99%

A comparative proteomic study on the effects of metal pollution in oysters Crassostrea hongkongensis

et al. 2016

Marine Pollution Bulletin

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The metal pollution has posed great risk on the coastal organisms along the Jiulongjiang Estuary in South China. In this work, two-dimensional electrophoresis-based proteomics was applied to the oysters Crassostrea hongkongensis from metal pollution sites to characterize the proteomic responses to metal pollution. Metal accumulation and proteomic responses indicated that the oysters from BJ site were more severely contaminated than those from FG site. Compared with those oyster samples from the clean site (JZ), metal pollution induced cellular injuries, oxidative and immune stresses in oyster heapatopancreas from both BJ and FG sites via differential metabolic pathways. In addition, metal pollution in BJ site induced disturbance in energy and lipid metabolisms in oysters. Results indicated that cathepsin L and ferritin GF1 might be the biomarkers of As and Fe in oyster C. hongkongensis, respectively. This study demonstrates that proteomics is a useful tool for investigating biological effects induced by metal pollution.

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Differential protein expression in two bivalve species; Mytilus galloprovincialis and Corbicula fluminea; exposed to Cylindrospermopsis raciborskii cells

Cited by 67 publications

References 56 publications

iTRAQ-based quantitative proteomic analyses on the gender-specific responses in mussel Mytilus galloprovincialis to tetrabromobisphenol A

iTRAQ-based quantitative proteomic analyses on the gender-specific responses in mussel Mytilus galloprovincialis to tetrabromobisphenol A

Proteomic and metabolomic analysis reveal gender-specific responses of mussel Mytilus galloprovincialis to 2,2′,4,4′-tetrabromodiphenyl ether (BDE 47)

A comparative proteomic study on the effects of metal pollution in oysters Crassostrea hongkongensis

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