Direct Diagnosis of &lt;b&gt;&lt;i&gt;Trichomonas vaginalis&lt;/i&gt;&lt;/b&gt; Infection on Archived Pap Smears Using Nested PCR

Hezarjaribi, Hajar Ziaei; Taghavi, Mahbobeh; Fakhar, Mahdi; Gholami, Shirzad

doi:10.1159/000369772

Cited by 8 publications

(6 citation statements)

References 24 publications

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“…This percentage was obtained by serology. 19 The frequency observed in pregnant patients in our population is higher than previously reported in Mexico.…”

Section: Discussioncontrasting

confidence: 65%

Sexually transmitted pathogens, coinfections and risk factors in patients attending obstetrics and gynecology clinics in Jalisco, Mexico

Casillas-Vega

Morfín‐Otero

García

et al. 2016

SALUD PÚBLICA DE MÉXICO

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Objective. To determine the frequency of nine sexually transmitted pathogens, coinfections and risk factors in patients attending obstetrics and gynecology clinics in Jalisco, Mexico. Materials and methods. Samples from 662 patients attending obstetrics and gynecology clinics were analyzed. Treponema pallidum, HIV, and HCV were detected by serology. HPV was detected by Polimerase Chain Reaction (PCR), and its genotype was determined by Restriction Fragment Length Polymorphism (RFLP). Trichomonas vaginalis, HSV-1, HSV-2, Mycoplasma genitalium, Neisseria gonorrhoeae and T. pallidum were detected by multiplex PCR. Results. By serology, HIV frequency was 6.8%, T. pallidum was 2.26%, and HCV was 0.15%. By PCR, HPV frequency was 13.9%, (more frequent genotype was 16, 33.7%), followed by T. vaginalis (14.2%), HSV-1 (8.5%), M. genitalium (2,41%), N. gonorrhoeae (2.11%), HSV-2 (1.8%), and T. pallidum (1.05%). Patients

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“…This percentage was obtained by serology. 19 The frequency observed in pregnant patients in our population is higher than previously reported in Mexico.…”

Section: Discussioncontrasting

confidence: 65%

Sexually transmitted pathogens, coinfections and risk factors in patients attending obstetrics and gynecology clinics in Jalisco, Mexico

Casillas-Vega

Morfín‐Otero

García

et al. 2016

SALUD PÚBLICA DE MÉXICO

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“…T. vaginalis grows best at alkaline environment and attaches itself by its axostyle thus creating the irritation, inflammation, and other symptoms associated with the infection by this parasite [24]. Although our result agreed with those of [25] who found that T.vaginalis may be found in any type of vaginal discharge .…”

Section: Discussionsupporting

confidence: 84%

Feasibility of a nested PCR for the diagnosis of Trichomonas vaginalis infection in women a Wasit Province, Iraq

Rahi

Jaleel

2022

ijhs

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This study was concerned the describe of an current PCR method for specific detection of Tichomonas vaginalis DNA into a rapid nested PCR assay, and to estimate the recent prevalence of T. vaginalis according to studying different factors that effect on vaginal trichomoniasis in women complaining of vaginal discharge. Vaginal swab samples were collected from 100 patients lived at Wasit province, during the period from January, 2022 to July, 2022. Demographic data, clinical and socioeconomic status were collected from the patients using a structured questionnaire. Total genomic DNA was isolated from 100 samples of vaginal swab randomly chosen to specific detection of T. vaginalis DNA into a rapid nested PCR. All samples were examined by the direct microscopic examination (wet mount). The vaginal swabs examined by wet mount and nested PCR to detect the presence of T. vaginalis. Of the 100 women, 29 were positive by nested PCR giving the prevalence rate of (29%) with and the direct microscopic examination (wet mount) showed 4/100 (4 %) positive case. Their ages were different. Women aged (20-30)years had significantly higher prevalence of trichomoniasis (14%) than other age groups.

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“…Then, the supernatant was precipitated with 400 μL of absolute ethanol. Finally, 50 μL sterile distilled water was added to precipitated DNA and stored at 4 °C until it could be tested for P. falciparum DNA [21]. …”

Section: Methodsmentioning

confidence: 99%

Genetic diversity at the C-terminal domain of knob-associated histidine-rich protein (KAHRP) of Plasmodium falciparum isolates from Burundi, Eastern Africa

Mardani

Hezarjaribi

Fakhar

et al. 2018

Annals of Medicine and Surgery

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The knob-associated histidine-rich protein (KAHRP) is an exported parasite protein and the major component of infected erythrocytes by Plasmodium falciparum. P. falciparum histidine-rich protein-1 (PfHRP-1) is docked by KAHRP, which this interaction plays a significant role in cytoadherence of the malaria protozoan to erythrocytes and pathogenicity. The most polymorphic region of the PfHRP-1 is the C-terminal of decapeptide repeat domain (region III). The main objective of this study was to explore the genetic diversity at the region III of KAHRP in P. falciparum isolates from Burundi. In the present study, the nested PCR was performed for the amplification of the coding gene (kahrp gene) for region III in 35 P. falciparum isolates from Burundi. The nested PCR products of seven randomly selected isolates were purified and then sequenced. As the result, three allelic forms (340 bp, 370 bp, and 400 bp) were seen at the C-terminal domain of kahrp gene. The existence of multiple alleles of the kahrp gene revealed the presence of different P. falciparum strains in Burundi. It is suggested that the results could be useful in designing and the improvement of targeted therapy agents for falciparum malaria.

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Direct Diagnosis of <b><i>Trichomonas vaginalis</i></b> Infection on Archived Pap Smears Using Nested PCR

Cited by 8 publications

References 24 publications

Sexually transmitted pathogens, coinfections and risk factors in patients attending obstetrics and gynecology clinics in Jalisco, Mexico

Sexually transmitted pathogens, coinfections and risk factors in patients attending obstetrics and gynecology clinics in Jalisco, Mexico

Feasibility of a nested PCR for the diagnosis of Trichomonas vaginalis infection in women a Wasit Province, Iraq

Genetic diversity at the C-terminal domain of knob-associated histidine-rich protein (KAHRP) of Plasmodium falciparum isolates from Burundi, Eastern Africa

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