Direct immobilization of antibodies on a new polymer film for fabricating an electrochemical impedance immunosensor

Zhang, Xiangyang; Shen, Guohua; Shen, Youming; Yin, Dan; Zhang, Chunxiang

doi:10.1016/j.ab.2015.06.007

Cited by 11 publications

(4 citation statements)

References 20 publications

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“…22 The presence of pyridine enhances the stability of antibody binding through the covalent bonding with -NH 2 blocks of the immobilized antibody. 23 Furthermore, deconvoluted N 1s spectra (at 399.7 eV and 401.7 eV) could be ascribed for pyridine nitrogen (-C = NH-, -C-N) which indicates the successful grafting of pyridine in the rGO flakes as shown in Fig. 2G.…”

Section: Physical Characterization Of Rgo/cho-mp-figuresmentioning

confidence: 85%

A Prostate Cancer Detection Immunosensor Based on Nafion/Reduced Graphene Oxide/Aldehyde Functionalized Methyl Pyridine Composite Electrode

Jeong¹,

Barman²,

Yoon³

et al. 2019

J. Electrochem. Soc.

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In this research, an electrochemical PSA (prostate specific antigen) immunosensor based on Nafion/reduced graphene oxide (rGO)/aldehyde methyl pyridine (CHO-MP) composite electrode was newly developed by using noncovalent functionalization of rGO and CHO-MP. Due to improper binding stability of the immobilized antibody, immunosensors may show false positive results. The proposed composite material can provide suitable microenvironment for the immobilized anti-PSA and improve the conductivity of electrode. Furthermore, the used nafion can improve the mechanical and chemical behaviors of the developed immunosensor. Physical characterization of rGO/CHO-MP was observed through XPS, FTIR, and FE-SEM. Under the optimized conditions, the fabricated immunosensor showed a linear range of 0.005-90 ng mL −1 , detection limit of 1.6 pg mL −1 , excellent selectivity, stability, and reproducibility. Lastly, human serum samples were analyzed and the recovery range was obtained as 95.8% to 107.9% with an RSD of 5.1% (n = 5). These results showed that the as-fabricated sensor is highly promising in clinical applications.

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Section: Physical Characterization Of Rgo/cho-mp-figuresmentioning

confidence: 85%

A Prostate Cancer Detection Immunosensor Based on Nafion/Reduced Graphene Oxide/Aldehyde Functionalized Methyl Pyridine Composite Electrode

Jeong¹,

Barman²,

Yoon³

et al. 2019

J. Electrochem. Soc.

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show abstract

“…137 Serving as a specific organic linker for carboxylic groups, ethyl (dimethylamino propyl) carbodiimide (EDC) 140 or ethyl (dimethylamino propyl) carbodiimide/Nhydroxysuccinimide (EDC/NHS) 135,137,144,178 is often included in an experiment to achieve activation and protection from carboxylic radicals. However, based on the conclusion of Zhang et al, 114 Liang et al 134 utilized graphene oxide nanoribbons/multi-walled carbon nanotubes (GONRs/ MWCNTs) hybrids to implant antigens without using linkage chemicals. Some polymers containing amino groups such as poly (1,5-diaminonaphthalene) (P(1,5-DAN)) 129 and polyaniline (PANI), 132 can bind to antibodies through a direct and glutaraldehyde (GA)-linked interaction, respectively.…”

Section: Electrochemical Monitoring Systemmentioning

confidence: 99%

Endocrine disrupting chemicals in water and recent advances on their detection using electrochemical biosensors

Wang

Tizaoui

et al. 2023

Sens. Diagn.

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“…To circumvent this limitation, some groups have proposed to adsorb polymers onto the electrode, typically ones carrying aldehyde groups. [ 2 ] The use of conductive polymers such as polyaniline is particularly promising because they provide a good platform for fast electron transfer. [ 3 ] Using a polymeric interface, however, doesn't necessarily avoid the use of linkage agents such as carbodiimide (EDC) and N‐hydroxysuccinimide (NHS) or glutaraldehyde, in which case the biofunctionalization remains a multistep process.…”

Section: Introductionmentioning

confidence: 99%

Plasma Deposited Polyoxazoline Thin Films for the Biofunctionalization of Electrochemical Sensors

Eulate

Gheorghiu

Amoura

et al. 2021

Adv Materials Technologies

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Electrochemical immunosensors are an emerging technology for the fast, sensitive, and reliable diagnosis of diseases from bodily fluids. These sensors work by detecting a change in current upon analyte binding to an immuno‐functionalized electrode. Current methods of electrode functionalization are lengthy processes involving self‐assembled monolayer formation and wet chemistry biofunctionalization. Herein, thin films deposited from the plasma phase of oxazoline precursors are investigated and optimized as an alternative approach for electrode functionalization. The plasma‐enabled method has the advantage of being substrate independent and allows the spontaneous binding of biomolecules in physiological buffer. Surface sensitive analysis techniques are employed to characterize the thickness, reactivity, and stability of the thin films before investigating their electrochemical properties on indium tin oxide and gold electrodes including the feasibility to reduce charge transfer resistance with gold nanoparticles. Last, these films are employed to develop an immunosensor for the detection of free epithelial cell adhesion molecule with a limit of detection of 8.7 ng mL−1.

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Direct immobilization of antibodies on a new polymer film for fabricating an electrochemical impedance immunosensor

Cited by 11 publications

References 20 publications

A Prostate Cancer Detection Immunosensor Based on Nafion/Reduced Graphene Oxide/Aldehyde Functionalized Methyl Pyridine Composite Electrode

A Prostate Cancer Detection Immunosensor Based on Nafion/Reduced Graphene Oxide/Aldehyde Functionalized Methyl Pyridine Composite Electrode

Endocrine disrupting chemicals in water and recent advances on their detection using electrochemical biosensors

Plasma Deposited Polyoxazoline Thin Films for the Biofunctionalization of Electrochemical Sensors

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