Direct proof of spontaneous translocation of lipid-covered hydrophobic nanoparticles through a phospholipid bilayer

Guo, Yachong; Terazzi, Emmanuel; Seemann, Ralf; Fleury, Jean‐Baptiste; Baulin, Vladimir A.

doi:10.1126/sciadv.1600261

Cited by 109 publications

(146 citation statements)

References 58 publications

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“…The propensity of biological species to form coronas around nanoparticles [1][2][3][4][5] has been used for preparing engineered nanomaterials that can be distributed in biological systems with some control. [6][7][8][9][10][11] Although protein coronas in particular have been studied extensively, 1-3,6,7 our understanding of lipid coronas is now just beginning to emerge, [12][13][14][15] especially of those formed upon unintended nanoparticle contacts with living cells. The protein and lipid corona formation mechanisms appear to differ substantially, as ''hard'' and ''soft'' coronas, 16 typical for the former, have not been described for the latter.…”

Section: Introductionmentioning

confidence: 99%

Lipid Corona Formation from Nanoparticle Interactions with Bilayers

Olenick

Troiano

Vartanian

et al. 2018

Chem

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Mechanisms of corona formation around nanomaterials remain enigmatic. Here, we provide evidence for spontaneous lipid corona formation that engenders new particle properties without the need for active mixing upon attachment to stationary and suspended lipid bilayer membranes. The mechanism of lipid corona formation can be used to improve control over nano-bio interactions and to help understand why some nanomaterial-ligand combinations are detrimental to organisms but others are not. SUMMARYAlthough mixing nanoparticles with certain biological molecules can result in coronas that afford some control over how engineered nanomaterials interact with living systems, corona formation mechanisms remain enigmatic. Here, we report results from experiments and computer simulations that provide concrete lines of evidence for spontaneous lipid corona formation without active mixing upon attachment to stationary and suspended lipid bilayer membranes. Experiments show that polycation-wrapped particles disrupt the tails of zwitterionic lipids, increase bilayer fluidity, and leave the membrane with reduced z potentials. Computer simulations suggest that the contact ion pairing between the lipid head groups and the polycations' ammonium groups leads to the formation of stable, albeit fragmented, lipid bilayer coronas. The mechanistic insight regarding lipid corona formation can be used to improve control over nanobio interactions and to help understand why some nanomaterial-ligand combinations are detrimental to organisms but others are not.

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Section: Introductionmentioning

confidence: 99%

Lipid Corona Formation from Nanoparticle Interactions with Bilayers

Olenick

Troiano

Vartanian

et al. 2018

Chem

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“…At the mesoscale (characteristic length ∼ 100 nm) , experimental studies have largely focused on the biodistribution of functionalized NPs, targeted to various cellular adhesion molecules both in vitro and in vivo, for use as targeted drug delivery systems [29][30][31][32][33][34][35][36]. A number of theoretical and computational models [37][38][39][40][41][42][43][44][45][46][47][48][49][50] have been developed to address the question of adhesion and super-selective targeting of functionalized NCs and their uptake into the target cell [51][52][53][54][55][56]. At the molecular scale (characteristic length < 20 nm), all atom molecular dynamics simulations have been extensively used to unravel the molecular mechanisms governing receptor-ligand interactions [57][58][59][60][61] and the interaction of gold and silver nanoparticles with the surface of a cell [62][63][64][65].…”

Section: Introductionmentioning

confidence: 99%

Multivalent Binding of a Ligand-Coated Particle: Role of Shape, Size, and Ligand Heterogeneity

McKenzie

Rammohan

et al. 2018

Biophysical Journal

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We utilize a multiscale modeling framework to study the effect of shape, size, and ligand composition on the efficacy of binding of a ligand-coated particle to a substrate functionalized with the target receptors. First, we show how molecular dynamics along with steered molecular dynamics calculations can be used to accurately parameterize the molecular-binding free energy and the effective spring constant for a receptor-ligand pair. We demonstrate this for two ligands that bind to the αβ-domain of integrin. Next, we show how these effective potentials can be used to build computational models at the meso- and continuum-scales. These models incorporate the molecular nature of the receptor-ligand interactions and yet provide an inexpensive route to study the multivalent interaction of receptors and ligands through the construction of Bell potentials customized to the molecular identities. We quantify the binding efficacy of the ligand-coated-particle in terms of its multivalency, binding free-energy landscape, and the losses in the configurational entropies. We show that 1) the binding avidity for particle sizes less than 350 nm is set by the competition between the enthalpic and entropic contributions, whereas that for sizes above 350 nm is dominated by the enthalpy of binding; 2) anisotropic particles display higher levels of multivalent binding compared to those of spherical particles; and 3) variations in ligand composition can alter binding avidity without altering the average multivalency. The methods and results presented here have wide applications in the rational design of functionalized carriers and also in understanding cell adhesion.

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“…This observation is in agreementw ith previous studies on lipid-coatedh ydrophobic Au NPs of 5nm, which demonstrated that hydrophobic NPs with diameter d > 5nmt ranslocate through the bilayer,w hereas individual NPs with d 5nma re trapped in the bilayer.T he only possibility of small hydrophobic NPs for leaving the bilayer is by forming clusters exceeding the threshold size. [36] Based on our previous work, we know that the QD_F form nanoclusters in aqueous media containing only 5% of DMSO. [37] Therefore our hypothesis is that these hydrophobic QDs, both QD_F and QD_TOPO, cross the cell membrane as clusters.…”

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confidence: 99%

Surface‐Active Fluorinated Quantum Dots for Enhanced Cellular Uptake

Argudo

Carril

Martín‐Romero

et al. 2018

Chemistry A European J

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Fluorescent nanoparticles, such as quantum dots, hold great potential for biomedical applications, mainly sensing and bioimaging. However, the inefficient cell uptake of some nanoparticles hampers their application in clinical practice. Here, the effect of the modification of the quantum dot surface with fluorinated ligands to increase their surface activity and, thus, enhance their cellular uptake was explored.

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Direct proof of spontaneous translocation of lipid-covered hydrophobic nanoparticles through a phospholipid bilayer

Abstract: Spontaneously translocating lipid-coated hydrophobic gold nanoparticles open doors for new biotechnology applications.

Cited by 109 publications

References 58 publications

Lipid Corona Formation from Nanoparticle Interactions with Bilayers

Lipid Corona Formation from Nanoparticle Interactions with Bilayers

Multivalent Binding of a Ligand-Coated Particle: Role of Shape, Size, and Ligand Heterogeneity

Surface‐Active Fluorinated Quantum Dots for Enhanced Cellular Uptake

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