Discordant Carbapenem Susceptibility in
            <b>
              <i>Methylobacterium</i>
              Species and Its Application as a Method for Phenotypic Identification
            </b>

Zaharatos, Gerasimos J.; Dascal, André; Miller, Mark A.

doi:10.1128/jcm.39.5.2037-2038.2001

Cited by 13 publications

(13 citation statements)

References 11 publications

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“…Discordant carbapenem susceptibilities, with high sensitivity to imipenem (MIC ϭ 0.25 to 1 mg/liter) and resistance to meropenem (MIC Ͼ 32 mg/liter), seem to be a distinctive feature of Methylobacterium spp. (13).…”

Section: Microbiology Laboratory Identification and Antibiotic Suscmentioning

confidence: 99%

Methylobacterium and Its Role in Health Care-Associated Infection

2014

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bMethylobacterium species are a cause of health care-associated infection, including infections in immunocompromised hosts. The ability of Methylobacterium species to form biofilms and to develop resistance to high temperatures, drying, and disinfecting agents may explain the colonization of Methylobacterium in the hospital environment in, e.g., endoscopes. Due to its slow growth, it can be easily missed during microbiological surveillance of endoscope reprocessing. The purpose of this minireview is to present an overview of documented infections and cross-contaminations with Methylobacterium related to endoscopic procedures and to illustrate the health care-associated relevance of this slow-growing bacterium.

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Section: Microbiology Laboratory Identification and Antibiotic Suscmentioning

confidence: 99%

Methylobacterium and Its Role in Health Care-Associated Infection

2014

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“…It might be possible for clinical microbiology laboratories to be able to infer the species identity of a Methylobacterium isolate from antibiotic sensitivity and thereby recommend an optimal regime of antibiotic treatment. Differential sensitivity has also been suggested as a means of phenotypic identification at the genus level of clinical isolates as Methylobacterium (Zaharatos et al 2001). This conjecture was based on differential sensitivity of four clinical isolates and ATCC cultures of Mtb extorquens, Mtb organophilum, and Mtb mesophilicum to imipenem (extreme susceptibility) and meropenem (high resistance).…”

Section: Methylobacterium and Animalsmentioning

confidence: 98%

“…In one example, intervention with five antibiotics was needed to cure the infection (Hornei et al 1999). Aminoglycoside antibiotics (imipenem, ticarcillin-clavulanic acid, sulfamethoxazole, and trimethoprim), tetracycline, rifamycin, and gentamycin have been reported to be effective against Methylobacterium, but a number of studies have shown resistance of some strains to antibiotics, including cephalosporins, ampicillin, piperacillin, carbenicillin, spectinomycin, puromycin, cefuroxime, erythromycin, vancomycin, chloramphenicol, fosfomycin, piperacillin, cefazolin, cefotetan, cerfuroxime, ceftazidime, ceftriaxone, ciprofloxacin, meropenem, nalidixic acid, teicoplanin, gentamycin, amikacin, nitrofurantoin, and p-fluorophenylalanine (Borsali et al 2011;Brown et al 1992;Furuhata et al 2006;Goodwin et al 1988;Korvick et al 1989;Lee et al 2004;Zaharatos et al 2001). Brown et al (1992) showed the majority of 15 clinical isolates of Methylobacterium were resistant to b-lactam drugs, with the exceptions of ceftriaxone and ceftizoxime, and that six of these were b-lactamase-negative with a minimum inhibitory concentration for ampicillin of 4 mg ml À1 , whereas nine b-lactamase-positive isolates were resistant to 8-32 mg ml À1 (or more).…”

Section: Methylobacterium and Animalsmentioning

confidence: 99%

The Family Methylobacteriaceae

Kelly¹,

McDonald²,

Wood³

2014

The Prokaryotes

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“…However, most isolates demonstrate high MICs for beta-lactam drugs, with the exception of imipenem, for which an MIC 90 of 1 g/ml has been reported (2,4). Interestingly, the reported Methylobacterium MIC 90 for meropenem is Ͼ32 g/ml, a feature that may aid in the differentiation of Methylobacterium from Roseomonas, as both species have low MICs for both carbapenems (5).…”

Section: T He Organism Was Identified As Methylobacterium Fujisawaensementioning

confidence: 99%

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et al. 2015

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c T he organism was identified as Methylobacterium fujisawaense. The pink-pigmented nonfermenters (PPNFs) include Asaia, Azospirillum, Methylobacterium, and Roseomonas species. Roseomonas colonies are typically larger than those of Methylobacterium, and Gram staining demonstrates nonvacuolated coccoid rods. Methylobacterium and Asaia spp. are the only PPNFs that are trypsin positive, a feature that distinguishes these species from Roseomonas. Methylobacterium can be differentiated from Asaia by the positive urease reaction of the former (1). Methylobacterium grows best at 25 to 30°C on Sabouraud agar (SAB) and is included in the databases of some automated identification systems; our isolate was identified as Methylobacterium sp. with 99% confidence by the Vitek 2 Gram-negative identification test (GN ID) card. However, caution should be used in interpreting biochemical reactions for suspected Methylobacterium, as phenotypic characteristics may vary (2).Partial 16S rRNA sequencing was performed, yielding 100% homology to M. fujisawaense. In addition, a sample taken from the SAB plate was analyzed by two different platforms of matrix-assisted laser desorption ionization-time of flight (MALDI-TOF) mass spectrometry (Bruker Daltonics [Billerica, MA] and bioMérieux [Durham, NC]). Both MALDI-TOF analyses identified the isolate as M. fujisawaense (with confidence levels of Ͼ2.0 with the Bruker system and 99.9 with the bioMérieux system). Currently, there are no standard antimicrobial susceptibility testing (AST) methods or interpretive criteria for Methylobacterium spp. We attempted broth microdilution (BMD) AST in cation-adjusted Mueller-Hinton broth, but growth of the isolate in this medium at both 30°C and 35°C was too poor to allow interpretation of AST results. Other researchers have tested Methylobacterium spp. by BMD in Mueller-Hinton broth and have noted low MICs for aminoglycosides, tetracyclines, and fluoroquinolones (3). However, most isolates demonstrate high MICs for beta-lactam drugs, with the exception of imipenem, for which an MIC 90 of 1 g/ml has been reported (2, 4). Interestingly, the reported Methylobacterium MIC 90 for meropenem is Ͼ32 g/ml, a feature that may aid in the differentiation of Methylobacterium from Roseomonas, as both species have low MICs for both carbapenems (5).Methylobacterium spp. are methylotrophs found ubiquitously in soil, sewage, plants, and fresh water. They are described in the literature as causes of contamination, colonization, and opportunistic infections (4). Several case reports document bloodstream infections caused by Methylobacterium species in immunocompromised patients, with these infections often associated with indwelling central venous catheters (CVCs). For our patient, the isolate was recovered only from the fungal blood culture, illustrating the mesophilic nature of this group of organisms. M. fujisawaense is described as a human pathogen only in a single case report, involving a 57-year-old female undergoing unrelated allogeneic hematopoietic stem cell trans...

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Discordant Carbapenem Susceptibility in Methylobacterium Species and Its Application as a Method for Phenotypic Identification

Cited by 13 publications

References 11 publications

Methylobacterium and Its Role in Health Care-Associated Infection

Methylobacterium and Its Role in Health Care-Associated Infection

The Family Methylobacteriaceae

Answer to May 2015 Photo Quiz

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