Disorders of reproductive function in female dogs and methods of diagnostic

Holumbiovska, T.V.; Stefanyk, V. Yu.

doi:10.15421/nvlvet8376

Cited by 5 publications

(5 citation statements)

References 7 publications

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“…Pathology of the dog reproductive tract is frequently diagnosed. It is characterized by prolonged anestrus or lack of heat (Holumbiovska & Stefanyk, 2018). Under such conditions, estrus induction is recommended (Concannon, 1989;Johnston et al, 2001;Walter et al, 2011).…”

Section: Fig 4 Non-nuclear Cells Dynamics During the Estrous Cycle In...mentioning

confidence: 99%

Effects of implant Suprelorin on cytological changes of vaginal epithelial cells in female dogs (4.7 mg deslorelin)

Holumbiiovska

Stefanyk

Basarab

2021

Ukr. Jour. of Vet. and Agr. Sci.

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Diagnosis of the estrous cycle in female dogs is important in veterinary medicine. The estrous cycle is divided into the following stages: proestrus, estrus, diestrus, metestrus, and anestrus, which due to the action of hormones, replace one after another. This feature of the estrous cycle leads to some difficulties with reproduction. It is hard to obtain newborns because of prolonged anestrus. Vaginal cytology is a simple and available diagnostic method of the estrous cycle. It is based on the analysis of vaginal mucosa epithelial cells changes. This method is used to determine the time of mating and diagnose pathological conditions in the reproductive system of female dogs. There are various drugs in veterinary medicine for the correction of the estrous cycle in female dogs. We used implant Suprelorin® to estrus induction. There were described methods of estrus stimulation using a subcutaneous implant Suprelorin (4.7 mg, deslorelin) and changes in vaginal epithelial cells using the vaginal cytology method. The study was performed on twelve (n = 12) adult female dogs in the anestrus stage. Implants were injected subcutaneously in the groin area and removed at the beginning of the estrus.

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Section: Fig 4 Non-nuclear Cells Dynamics During the Estrous Cycle In...mentioning

confidence: 99%

Effects of implant Suprelorin on cytological changes of vaginal epithelial cells in female dogs (4.7 mg deslorelin)

Holumbiiovska

Stefanyk

Basarab

2021

Ukr. Jour. of Vet. and Agr. Sci.

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“…Due to the fact that the majority of inconclusive relationships are associated with the incorrect determination of the ovulation period, the evaluation of the condition of the ovaries is important in the diagnosis of the optimal time of bitch insemination (Radokhlib & Krajewski, 2014;Holumbiovska & Stefanyk, 2018). Ultrasound examination is a perspective and an effective method of monitoring the reproductive system organs (Kähn & Volkmann, 2004;Mantziaras & Luvoni, 2020;Vlasenko et al, 2021).…”

Section: Introductionmentioning

confidence: 99%

Advantages and difficulties of ultrasound analysis to determine the fertile period in bitches

Holubiev,

Skliarov,

Pérez-Marín

et al. 2023

TAVM

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Definition the fertile period is one of the most important factors contributing to fertilization. Therefore, this analysis is important to solve the problem of infertility. The methods that available today do not provide the expected result, because the vast majority of unsuccessful mating result are related to the incorrect determination of the ovulation period. Therefore, it is important to assess the condition of the ovaries, and the prospect is ultrasound examination, which is an effective method of monitoring the reproductive organs. Ultrasound analysis is a non-invasive and effective method to establish the fertile period in bitches which makes it possible to track changes in the ovaries, detect follicles, their number, and determine the true (morphological) signs of ovulation. Clinicalvisual and hormonal studies which are based on the indirect determination of the optimal period of female insemination have the advantages in compare with vaginal cytology. Particularly, the use of ultrasound in real time allows to visualize the morphological changes of the ovaries and uterus -the irregular shape of the ovaries with large (0.6-1.2 cm) anechoic structures of a rounded or oval shape with a thin capsule, thickening of the endometrium (up to 0.5-0.8 cm) with a hypoechoic structure of the uterine horns. However, in our research we encountered certain difficulties, which turned out to be the same as other authors. Echography of the ovaries can seem a bit complicated, because the follicles that persist in them have a picture similar to that before and after ovulation. Some ovulated follicles do not always completely collapse (fall) during ovulation and are gradually replaced by luteal tissue while maintaining an echogenic picture on the days of the next ovulation. Non-ovulated follicles can complicate sonography interpretation. To diagnose ovulation, an ultrasound analysis should be performed twice a day, which is inconvenient for the owners. But if the ultrasound test is carried out less often than aforementioned number, there is a risk to miss the moment of ovulation, because the follicles before and after ovulation are very similar. An even more significant problem is the location of the ovaries in bitches -costovertebral angle surrounded by fat and an ovarian sac, which can create technical difficulties. In addition, this diagnostic approach may be more difficult in fat or very large dogs, as well as in dogs with thick skin (Shar Pei, Newfoundland).

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“…Impaired reproductive function is an urgent problem not only in veterinary medicine but in human medicine also (Cederroth et al, 2010;Mascarenhas et al, 2012;Bidne et al, 2018). Infertility is widespread for all species of animals and causes significant economic losses to livestock due to the loss and under-production of the offspring, reduced viability and, consequently, increased morbidity and mortality (Pasynkova, 2010;Golumbijovs'ka & Stefanyk, 2018;Medvedskij & Mazolo, 2018).…”

Section: Introductionmentioning

confidence: 99%

Effect of abiotic factors on sulfidogenic activity of bacteria Desulfuromonas sp.

Сhaіka

Peretyatko

2020

Regul. Mech. Biosyst.

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Sulfur-reducing bacteria are promising agents for the development of new methods of wastewater treatment with the removal of ions of heavy metals and organic compounds. Study of the effect of various environmental factors on the growth and sulfidogenic activity of sulfur-reducing bacteria allows one to investigate the adaptability of these microorganisms to stress factors. The paper deals with the effect of рН, different concentrations of elemental sulfur, hydrogen sulfide and presence of various electron acceptors on the growth and sulfidogenic activity of bacteria Desulfuromonas sp. YSDS-3. The calculation of C/S ratio for sulfur-reducing bacteria Desulfuromonas sp. YSDS-3 was made, with the comparison with similar parameters of sulfate-reducing bacteria. In the medium with elemental sulfur, concentration of hydrogen sulfide increased with the concentration of elemental sulfur. Bacteria Desulfuromonas sp. YSDS-3 accumulated their biomass in the most effective way at the concentration of elemental sulfur of 10–100 mM. In the medium with polysulfide form of sulfur at the neutral pH, bacteria produced hydrogen sulfide and accumulated biomass the best. Hydrogen sulfide at the concentration of 3 mM did not inhibit the bacterial growth, but further increase in the hydrogen sulfide concentration inhibited the growth of bacteria. The bacteria did not grow at the hydrogen sulfide concentration of 25 mM and above. As the concentration of elemental sulfur and cell density increases, sulfidogenic activity of the bacteria grows. Presence of two electron acceptors (S and K2Cr2O7, S and MnO2, S and Fe (III)) did not affect the accumulation of biomass of the bacteria Desulfuromonas sp. YSDS-3. However, under such conditions the bacteria accumulated 1.5–2.5 times less hydrogen sulfide than in the test medium. After 12–24 h of cultivation, different concentrations of elemental sulfur had a significant effect on the sulfidogenic activity. However, during 3–16 days of cultivation, the percentage of effect of elemental sulfur concentration decreased to 31%, while the percentage of effect of cell density increased threefold. Presence in the medium of the electron acceptors (Cr (VI), MnO2, Fe (III)) alternative to elemental sulfur led to a significant decrease in the content of hydrogen sulfide produced by sulfur-reducing bacteria.

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Disorders of reproductive function in female dogs and methods of diagnostic

Cited by 5 publications

References 7 publications

Effects of implant Suprelorin on cytological changes of vaginal epithelial cells in female dogs (4.7 mg deslorelin)

Effects of implant Suprelorin on cytological changes of vaginal epithelial cells in female dogs (4.7 mg deslorelin)

Advantages and difficulties of ultrasound analysis to determine the fertile period in bitches

Effect of abiotic factors on sulfidogenic activity of bacteria Desulfuromonas sp.

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