Distinguishment of populated metastatic cancer cells from primary ones based on their invasion to endothelial barrier by biosensor arrays fabricated on nanoroughened poly(methyl methacrylate)

Nikshoar, Mohammad Saeed; Khosravi, Shahla; Jahangiri, Mojtaba; Zandi, Ashkan; Miripour, Zohreh Sadat; Bonakdar, Shahin; Abdolahad, Mohammad

doi:10.1016/j.bios.2018.07.036

Cited by 15 publications

(13 citation statements)

References 34 publications

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“…For example, core shell PMMA particles were covalently functionalized with porphyrin showed anticancer action (Ballestri et al,201). PMMA particles have been shown to inhibit metastatic cancer cells [22]. Moreover, curcuminoid-loaded PMMA particles have been reported to inhibit the cancer cell proliferation [23].…”

Section: Discussionmentioning

confidence: 99%

“…PMMA is basically biocompatible polyester with high resistance to chemical hydrolysis, and high drug permeability and most important is that it does not cause toxicity [19]. Recently, a few studies have been demonstrated that different forms and structures of PMMA possess anti-cancer properties [20][21][22][23][24]. There is no report on the effect of PMMA (400-500 mm) on cancer cell viability and so considering various beneficial attributes of PMMA, we have decided to study the effect of PMMA on cancer cells.…”

Section: Introductionmentioning

confidence: 99%

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Targeted delivery of poly (methyl methacrylate) particles in colon cancer cells selectively attenuates cancer cell proliferation

Khan

Akhtar

Almohazey

et al. 2019

Artificial Cells, Nanomedicine, and Biotechnology

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Poly (methyl methacrylate) (PMMA) is basically biocompatible polyester with high resistance to chemical hydrolysis, and high drug permeability and the most important characteristics of PMMA is that it does not produce any toxicity. There is not much information about PMMA action on the colon cancer cells. In the present study, we have synthesized PMMA nanoparticles. The distribution pattern of PMMA particles was analysed by Zeta sizer and the size of the particles was calculated by using quasi elastic light scattering (QELS). The surface structure and the morphology of PMMA were characterized by transmission electron microscope (TEM) and scanning electron microscope (SEM), respectively. We have also analysed their effects on cancerous cells (human colorectal carcinoma cells, HCT-116) and normal, healthy cells (human embryonic kidney cells, HEK-293) by using morphometric, MTT, DAPI and wound healing methods. We report that PMMA particles inhibited the cancer cell viability in a dose-dependent manner. The lower dose (1.0 lg/ml) showed a moderate decrease in cancer cell viability, whereas higher dosages (2.5 lg/ml, 5.0 lg/mL and 7.5 lg/mL) showed steadily decrease in the cancer cell viability. We also report that PMMA is highly selective to cancerous cells (HCT-116), as we did not find any action on the normal healthy cells (HEK-293). In conclusion, our results suggest PMMA particles are potential biomaterials to be used in the treatment of colon cancer.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Targeted delivery of poly (methyl methacrylate) particles in colon cancer cells selectively attenuates cancer cell proliferation

Khan

Akhtar

Almohazey

et al. 2019

Artificial Cells, Nanomedicine, and Biotechnology

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“…Nikshoar et al designed an impedance sensor on PCB for detection of populated metastatic cancer cells from primary ones based on a recently reported metastatic diagnosis assay [47]. As shown in Fig.…”

Section: Lab-on-pcb For Detecting Cell Viabilitymentioning

confidence: 99%

The review of Lab‐on‐PCB for biomedical application

et al. 2020

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Prevention of infectious diseases, diagnosis of diseases, and determination of treatment options all rely on biosensors to detect and analyze biomarkers, which are usually divided into four parts: cell analysis, biochemical analysis, immunoassay, and molecular diagnosis. However, traditional biosensing devices are expensive, bulky, and require a lot of time to detect, which also limited its application in resource‐limited areas. In recent years, Lab‐on‐PCB, which combines biosensing technology and PCB technology, has been widely used in biomedical applications due to its high integration, personalized design, and easy mass production. Among these Lab‐on‐PCB sensing devices, the PCB circuit plays an important role. It can be directly used as a resistance sensor to count cells, and also used as a control device to automatically control the detection device. Flexible PCBs can be used to make wearable medical biosensors. In addition, due to the high degree of integration of the PCB circuit, Lab‐on‐PCB can perform multiple inspections on the same platform, which reduces the inspection time equivalently. Therefore, in this review paper, we discuss the application of Lab‐on‐PCB in four analysis methods of cell analysis, biochemical analysis, immunoassay, and molecular diagnosis, and give some suggestions for improvement and future development trends at the end.

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“…Up to now, lab-on-PCBs include several biomedical applications, for example, for detecting cell viability [ 7 ], molecular diagnosis [ 8 ], organotypic cultures [ 9 ], or electrolytes detection [ 10 ]. Focusing on electrophoresis-based applications, capillary electrophoresis (CE) is the most usual technique in lab on chip [ 11 ].…”

Section: Introductionmentioning

confidence: 99%

Semi-Automatic Lab-on-PCB System for Agarose Gel Preparation and Electrophoresis for Biomedical Applications

2021

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In this paper, a prototype of a semi-automatic lab-on-PCB for agarose gel preparation and electrophoresis is developed. The dimensions of the device are 38 × 34 mm2 and it includes a conductivity sensor for detecting the TAE buffer (Tris-acetate-EDTA buffer), a microheater for increasing the solubility of the agarose, a negative temperature coefficient (NTC) thermistor for controlling the temperature, a light dependent resistor (LDR) sensor for measuring the transparency of the mixture, and two electrodes for performing the electrophoresis. The agarose preparation functions are governed by a microcontroller. The device requires a PMMA structure to define the wells of the agarose gel, and to release the electrodes from the agarose. The maximum voltage and current that the system requires are 40 V to perform the electrophoresis, and 1 A for activating the microheater. The chosen temperature for mixing is 80 ∘C, with a mixing time of 10 min. In addition, the curing time is about 30 min. This device is intended to be integrated as a part of a larger lab-on-PCB system for DNA amplification and detection. However, it can be used to migrate DNA amplified in conventional thermocyclers. Moreover, the device can be modified for preparing larger agarose gels and performing electrophoresis.

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Distinguishment of populated metastatic cancer cells from primary ones based on their invasion to endothelial barrier by biosensor arrays fabricated on nanoroughened poly(methyl methacrylate)

Cited by 15 publications

References 34 publications

Targeted delivery of poly (methyl methacrylate) particles in colon cancer cells selectively attenuates cancer cell proliferation

Targeted delivery of poly (methyl methacrylate) particles in colon cancer cells selectively attenuates cancer cell proliferation

The review of Lab‐on‐PCB for biomedical application

Semi-Automatic Lab-on-PCB System for Agarose Gel Preparation and Electrophoresis for Biomedical Applications

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