Diversity within Serogroups of Rhizobium leguminosarum biovar viceae in the Palouse Region of Eastern Washington as Indicated by Plasmid Profiles, Intrinsic Antibiotic Resistance, and Topography

Abstract

Serology, plasmid profiles, and intrinsic antibiotic resistance (IAR) were determined for 192 isolates of Rhizobium leguminosarum biovar viceae from nodules of peas (Pisum sativum L.) grown on the south slope and bottomland topographic positions in eastern Washington State. A total of 3 serogroups and 18 plasmid profile groups were identified. Nearly all isolates within each plasmid profile group were specific for one of the three serogroups. Cluster analysis of IAR data showed that individual clusters were dominated by one serogroup and by one or two plasmid profile groups. Plasmid profile analysis and IAR analysis grouped 72% of the isolates similarly. Most plasmid protile groups and several IAR clusters favored either the south slope or the bottomland topographic position. These findings show that certain intraserogroup strains possess a greater competitiveness for nodulation and/or possess a greater ability to survive in adjacent soil environments.

Symbiotic competence, genetic diversity and plasmid profiles of Egyptian isolates of a Rhizobium species from Leucaena leucocephala (Lam.) Dewit

“…There appears to be more variability in plasmid DNA content than in genomic DNA sequences as a whole. This is not surprising since Brockman and Bezdicek (1989) documented considerable variation in the plasmid profiles of field isolates of Rh. leguminosarum hv.…”

Rhizobia of chickpea from southern Portugal: symbiotic efficiency and genetic diversity

“…(1998). The strain Rhizobium leguminosarum biovar viciae T83K3 was used as standard for determination of plasmid molecular weight (Brockman and Bezdicek 1989).…”

Genotypic and Phenotypic Comparisons of Chromosomal Types within an Indigenous Soil Population of Rhizobium leguminosarum bv. trifolii

“…Serotyping allows researchers to gain a superficial picture of the diversity within native rhizobial populations at specific sites, to track the occurrence and distribution of antigenically related bacteria across states, countries, and continents, and to study the autecology of these bacteria in soil (4,7,13,15,17,30,33,36,38,40,42,49,53). Studies carried out with Bradyrhizobium japonicum (24,34,35,43,50,51,54) and Rhizobium leguminosarum (8,19,55) have shown that individual serotypes can be composed of nonidentical strains. As a result, concerns have been raised about the limitations of serological approaches to address various rhizobial phenomena of potential agricultural and ecological significance.…”