DNA damage in lymphocytes of patients suffering from complex traumatization

Bergholz, Liana M; Subič-Wrana, Claudia; Heylmann, Daniel; Beutel, Manfred E.; Wiltink, Jörg; Kaina, Bernd

doi:10.1016/j.dnarep.2017.02.006

Cited by 5 publications

(7 citation statements)

References 47 publications

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“…As such, lipid peroxidation might persist and constitute a long-term marker of stress experiences, while oxidative DNA damages might rather be observable in association with acute stress experiences or it might take more severe levels of psychological and oxidative stress to induce persistent oxidative DNA damages. Consistent with this hypothesis, it was recently reported that adult psychiatric patients with a history of complex childhood traumatization presented significantly higher levels of nuclear DNA damage in PBMC as assessed by γH2AX staining compared to healthy individuals and also compared to psychiatric patients with low levels of complex childhood traumatization (32). Complex childhood traumatization was here defined as the experience of sexual, physical or emotional abuse by a primary caregiver or another member of the family or social group the victim belongs to (32).…”

Section: Discussionmentioning

confidence: 56%

“…Consistent with this hypothesis, it was recently reported that adult psychiatric patients with a history of complex childhood traumatization presented significantly higher levels of nuclear DNA damage in PBMC as assessed by γH2AX staining compared to healthy individuals and also compared to psychiatric patients with low levels of complex childhood traumatization (32). Complex childhood traumatization was here defined as the experience of sexual, physical or emotional abuse by a primary caregiver or another member of the family or social group the victim belongs to (32). Investigating refugees with a high traumatic load, we also reported that individuals with PTSD showed higher levels of basal DNA breakage in PBMC compared to trauma-exposed subjects without PTSD and non-trauma-exposed control subjects (21).…”

Section: Discussionmentioning

confidence: 56%

“…With regard to CM, findings have however been inconsistent so far: While do Prado et al (31) reported that CM was associated with higher plasma levels of oxidative-stress-related protein carbonylation and an imbalance between oxidative molecules and antioxidants, Fanning et al (23) found no significant association between CM and plasma levels of oxidative stress biomarkers (8-OH(d)G and 8-isoprostane) in individuals with different personality disorders. Additionally, Bergholz et al (32) recently showed an association between complex childhood traumatization and nuclear DNA damage (γH2AX staining) in peripheral blood lymphocytes, while Simsek et al (33) previously reported that children with a history of childhood sexual abuse did not differ in serum levels of antioxidant enzymes, the antioxidant coenzyme Q, and DNA damage (8-OH(d)G) from children without such experiences.…”

Section: Introductionmentioning

confidence: 99%

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The Association of Childhood Maltreatment With Lipid Peroxidation and DNA Damage in Postpartum Women

et al. 2019

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Childhood maltreatment (CM) is associated with an increased risk for the development of psychiatric and somatic disorders in later life. A potential link could be oxidative stress, which is defined as the imbalance between the amount of reactive oxygen species (ROS) and the neutralizing capacity of anti-oxidative defense systems. However, the findings linking CM with oxidative stress have been inconsistent so far. In this study, we aimed to further explore this association by investigating biological markers of DNA and lipid damage due to oxidation in a comprehensive approach over two study cohorts of postpartum women (study cohort I and study cohort II). The severity of CM experiences (maltreatment load) was assessed in both studies using the Childhood Trauma Questionnaire . In study cohort I ( N = 30), we investigated whether CM was associated with higher levels of structural DNA damage in peripheral blood mononuclear cells (PBMC) by two methods that are highly sensitive for detecting nuclear DNA strand breaks (comet assay and γH2AX staining). In study cohort II ( N = 117), we then assessed in a larger cohort, that was specifically controlled for potential confounders for oxidative stress measurements, two established serum and plasma biomarkers of oxidative stress, one representing oxidative DNA and RNA damage (8-hydroxy-2′-deoxyguanosine and 8-hydroxyguanosine; 8-OH(d)G) and the other representing lipid peroxidation (8-isoprostane). In study cohort I, the analyses revealed no significant main effects of maltreatment load on cellular measures of nuclear DNA damage. The analyses of peripheral oxidative stress biomarkers in study cohort II revealed a significant main effect of maltreatment load on free 8-isoprostane plasma levels, but not on total 8-isprostane plasma levels and 8-OH(d)G serum levels. Taken together, by combining different methods and two study cohorts, we found no indications for higher oxidative DNA damages with higher maltreatment load in postpartum women. Further research is needed to investigate whether this increase in free 8-isoprostane is a marker for oxidative stress or whether it is instead functionally involved in ROS-related signaling pathways that potentially regulate inflammatory processes following a history of CM.

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Section: Discussionmentioning

confidence: 56%

Section: Discussionmentioning

confidence: 56%

Section: Introductionmentioning

confidence: 99%

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The Association of Childhood Maltreatment With Lipid Peroxidation and DNA Damage in Postpartum Women

et al. 2019

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“…The measurement of DNA damage by immunofluorescence out of peripheral blood lymphoid cells (PBLC) quantified the γ-H2AX (foci/cell) in the Institute for Toxicology of the University Medical Center Mainz, Germany [10,[28][29][30]. In short, the fresh EDTA venous blood samples were first incubated at 37°C for 1 h in the dark.…”

Section: Methodsmentioning

confidence: 99%

Pilot Study on Malnutrition and DNA Damage in Patients with Newly Diagnosed Gastrointestinal Tumors: Is DNA Damage Reversible by Early Individualized Nutritional Support?

Bergholz

Grimminger

Dikeoulia

et al. 2020

JGLD

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Background and Aims: Nutritional support (NS) in patients with malignancies and malnutrition improves outcome and treatment tolerance. The underlying mechanisms are not completely understood. We aimed to investigate for the first time the influence of an early individualized NS in newly diagnosed patients with gastrointestinal/hepato-pancreatic malignancies and malnutrition on DNA damage, oxidative stress and subclinical inflammation. Methods: This prospective case-control study included 43 patients with newly diagnosed malignancies and malnutrition. At baseline (F0), we documented patients’ data, oncological diagnosis, comorbidities, alcohol/ nicotine consume. Nutritional parameters, DNA damage [histone-variant H2AX phosphorylated on the 139-serine residue (γ-H2AX) foci/cell], oxidative status, subclinical inflammation were measured. During diagnostic workup, patients received an individualized NS, and got a follow-up before the start of treatment (F1), (n=21). Healthy controls (n=21) were included for comparison of DNA damage at baseline. Results: γ-H2AX-values at baseline were higher than in controls (p<0.001) and higher than after the NS at F1 (p=0.011). Patients with severe gastrointestinal symptoms (SGS) had higher baseline foci compared to patients with mild gastrointestinal symptoms (MGS) at F0 (p<0.001) and showed a stronger decrease of DNA damage under NS (p=0.002). Laboratory data were stable, with tendential reduction in oxidative stress, without progression of subclinical inflammation. The number of γ-H2AX foci did not differ among patients divided by sex, age, nicotine or alcohol intake or the presence of distant metastases. Conclusion: Increased baseline DNA damage in patients with newly diagnosed tumors and malnutrition decreased under pretherapeutic NS, independent of other known genotoxic factors. This contributes towards understanding the positive effects of early NS in cancer management.

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“…Psychology, 10, 2026Psychology, 10, -2037 https://doi.org/10.4236/psych.2019.1015130 health. For example, a severity of emotional stress modifies the processes of DNA damaging and reparation which directly related to carcinogenesis (Seredenin, Durnev, & Vedernikov, 1980;Markkanen, Meyer, & Dianov, 2016;Bergholz et al, 2017;Fink, 2019). For people in the state of emotional disadaptation, the genome sensitivity to genotoxic compounds-mutagens and/or carcinogens increases Ingel et al, 2010Ingel et al, , 2018.…”

Section: Introductionmentioning

confidence: 99%

Children’s Genomic Instability in Association with Balance of the Autonomous Nerve System Departments

Ingel¹,

Legostaeva²,

Krivtsova³

et al. 2019

PSYCH

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This article presents new results of the investigation of anxiety and balance of the departments of autonomic nervous system of 178 healthy 5 -7 years old children living in 2 different districts of Magnitogorsk town. It is shown that the state of increased and high anxiety more often was detected among children living around the Magnitogorsk Iron and Steel Combine (MISC). Among these children, an imbalanced work of the departments of autonomic nervous system (mostly ergotropic tone) found out more often than among children, living in the other district of the town. The increased genome instability as well as individual sensitivity of children's genome to standard genotoxic load determined in vitro in peripheral blood cultures (micronucleus test with Cytochalasin B) correlated with children's psychosomatic characteristics, especially for those who lived around the MISC.

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DNA damage in lymphocytes of patients suffering from complex traumatization

Cited by 5 publications

References 47 publications

The Association of Childhood Maltreatment With Lipid Peroxidation and DNA Damage in Postpartum Women

The Association of Childhood Maltreatment With Lipid Peroxidation and DNA Damage in Postpartum Women

Pilot Study on Malnutrition and DNA Damage in Patients with Newly Diagnosed Gastrointestinal Tumors: Is DNA Damage Reversible by Early Individualized Nutritional Support?

Children’s Genomic Instability in Association with Balance of the Autonomous Nerve System Departments

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