DPPH Free Radical Scavenger Components from the Fruits of Alpinia rafflesiana Wall. ex. Bak. (Zingiberaceae)

Mohamad, Habsah; Abas, Faridah; Permana, Dharma; Lajis, Nordin Hj; Ali, Abdul Manaf; Sukari, Mohd Aspollah; Hin, Taufiq Yap Yun; Kikuzaki, Hiroe; Nakatani, Nobuji

doi:10.1515/znc-2004-11-1208

Cited by 66 publications

(49 citation statements)

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“…1,1-Diphenyl-2-picrylhydrazyl radical (DPPH) activity Radical scavenging activity using >1,1-diphenyl-2-picrylhydrazyl radical (DPPH) was carried out according to protocols described by Mohamad et al (2004). 100 l of substock solution (in MeOH) of the extracts at concentrations 250, 125, and 62.5 g/ml were added into 96-well microplates.5 l of DPPH reagent (5 mg of DPPH in 2 ml of MeOH) was then added into each well and the solution was kept in the dark for 30 min.…”

Section: Total Phenolic Content (Tpc)mentioning

confidence: 99%

Chemical characterization and antioxidant activity of three medicinal Apiaceae species

Maulidiani

Abas

Khatib

et al. 2014

Industrial Crops and Products

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a b s t r a c tCentella asiatica ('Pegaga' = Malaysia) is well known plant that has been used as one of the ingredients in pharmaceutical and cosmetic industries. Using liquid chromatography with diode array and electrospray ionization/mass spectrometry (LC-DAD-ESI/MS) analysis, chemical profiling of three different Pegaga extracts (C. asiatica, H. bonariensis and H. sibthorpioides) revealed variations in their metabolite profile. Our findings showed that triterpenes of C. asiatica were characterized by the ursane-type triterpenes (madecassoside, asiaticoside, madecassic acid, and asiatic acid), while the two Hydrocotyle species consisted of oleanane-type triterpenes (barrigenol derivatives). Other variations are due to the difference in phenolic and flavonoid constituents. The three Pegaga extracts were also evaluated for their total phenolic content (TPC), 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radicals, and xanthine oxidase inhibition (XOI) activities. The results showed that C. asiatica has the most potent antioxidant activity (TPC = 72.09 mg/100 g DW; DPPH = 72.99 g/ml; XOI = 87.68 g/ml) as compared to H. bonariensis (TPC = 28.55 mg/100 g DW; DPPH = 22.43 g/ml; XOI = 32.23 g/ml) and H. sibthorpioides (TPC = 56.23 mg/100 g DW; DPPH = 37.86 g/ml; XOI = 14.73. g/ml).

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Section: Total Phenolic Content (Tpc)mentioning

confidence: 99%

Chemical characterization and antioxidant activity of three medicinal Apiaceae species

Maulidiani

Abas

Khatib

et al. 2014

Industrial Crops and Products

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“…A importância que se tem dado aos tocotrienóis deve-se às suas atividades biológicas. Há estudos que sugerem a inibição do crescimento de células cancerígenas em glândulas mamárias e redução do risco de doenças cardíacas por essas moléculas, que podem também prevenir outras patologias que se originam de estresse oxidativo (Nakagawa et al, 2007;Shibata et al, 2008).…”

Section: Resultados E Discussõesunclassified

“…A fase móvel constituiu-se de hexano:acetato de etila:ácido fórmico (85:20:3). Uma solução alcoólica de DPPH (2,2-difenil-1-picril-hidrazila) 0,2% foi utilizada para revelação somente em uma pequena faixa de uma das extremidades da placa, permitindo a visualização da região onde se encontrava a substância antioxidante (Mohamad et al, 2004). Removeu-se a sílica dessa região e para separá-la do composto, o resíduo foi solubilizado em clorofórmio e filtrado em funil sinterizado G3.…”

Section: Isolamento E Identificação Do Tocotrienolunclassified

Identificação de δ tocotrienol e de ácidos graxos no óleo fixo de urucum (Bixa orellana Linné)

Costa

Silva

Lordello

et al. 2013

Rev. bras. plantas med.

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Bixa orellana é uma planta nativa do Brasil, conhecida popularmente como urucum. O objetivo desse estudo foi extrair o óleo das sementes de B. orellana e, a partir dele, isolar e identificar o tocotrienol e quantificar seus ácidos graxos. A extração do óleo realizou-se em aparelho Soxhlet utilizando hexano como solvente extrator. O tocotrienol foi isolado por meio de métodos cromatográficos e identificado por espectrometria de ressonância magnética nuclear RMN ¹H e RMN 13C. Os ácidos graxos foram quantificados por cromatografia gasosa acoplada ao espectrômetro de massa. Os resultados demonstraram a presença de δ -tocotrienol e, dentre os ácidos graxos, a ocorrência do ácido aracdônico, que até o presente momento não havia sido relatada na espécie em estudo.

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“…For example, prior to the report by Abdul Malek et al [43], Alpinia mutica was previously reported to have inhibitory activity towards lipid oxidation [50] and anti-bacterial effects against Bacillus subtilis and methicillin-resistant Staphylococcus aureus (MRSA) [50] in addition to anti-platelet aggregation activities [51].…”

Section: Ht29 and Ccd-18comentioning

confidence: 99%

Studies of Malaysian Plants in Prevention and Treatment of Colorectal Cancer

Hashim¹,

Gill²,

Latimer³

et al. 2016

Colorectal Cancer - From Pathogenesis to Treatment

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Incidence rates vary 10-fold globally for colorectal cancer (CRC). Asia has lower rates than Western countries, but as the Western life-style becomes more prevalent in economically developing Asian countries, rates are increasing. Clinical therapy has improved over the last few decades, and national screening programmes are a proven and effective means of reducing mortality; chemoprevention through diet and life-style choices may provide additional value. Diet has strong associations with the aetiology of CRC, considerable epidemiological evidence exist that fruits and vegetables are associated with reduced risk of CRC. There is also extensive experimental evidence that phytochemicals from fruit and vegetables can modulate pathways of carcinogenesis. In this chapter, we consider Malaysia specifically, with its rich ethnopharmacological heritage and megabiodiversity; Malaysian natural compounds may be a source of potentially chemo-protective with relevance to CRC.

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DPPH Free Radical Scavenger Components from the Fruits of Alpinia rafflesiana Wall. ex. Bak. (Zingiberaceae)

Cited by 66 publications

References 0 publications

Chemical characterization and antioxidant activity of three medicinal Apiaceae species

Chemical characterization and antioxidant activity of three medicinal Apiaceae species

Identificação de δ tocotrienol e de ácidos graxos no óleo fixo de urucum (Bixa orellana Linné)

Studies of Malaysian Plants in Prevention and Treatment of Colorectal Cancer

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