Duck egg yolk in extender improves the freezability of buffalo bull spermatozoa

Andrabi, S. M. H.; Ansari, Muhammad Tayyab; Ullah, Nemat; Anwar, Muhammad; Abid, Muhammad; Akhter, Shamim

doi:10.1016/j.anireprosci.2007.07.003

Cited by 73 publications

(55 citation statements)

References 17 publications

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“…Ice crystal formation caused sperm cells injured and being damage to the cytoplasmic fracture; negative effects on the cytoskeleton and genome related structures; negative effect on sperm tail and its motile ability as well as reducing the fertility due to compromising the integrity of plasma membrane (Harshan et al, 2005;Andrabi et al, 2008). Cold shock influences the ratio of cholesterol/phospholipids, the content of lipids in the bilayer membrane, degree of hydrocarbon chain saturation and ratio of protein/phospholipid on the plasma membrane (Medeiros et al, 2002), reduces membrane permeability to water and solutes and injures acrosomal membranes (Purdy 2006), as well as mitochondrial sheath and axoneme (Salamon & Maxwell, 2000).…”

Section: Resultsmentioning

confidence: 99%

The Success Rate of Artificial Insemination Using Post-Thawed Spotted Buffaloes Epididymal Sperm

Yulnawati¹,

Maheshwari²,

Rizal³

et al. 2013

Med Pet

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Spotted buffalo, an exotic species that exists in Tana Toraja, South Sulawesi, Indonesia, is getting extinct due to high number of slaughtered during a funeral ceremony, called Rambu Solo', as well as special treatments that do not allow the male spotted buffaloes perform natural mating activity. According to that, the research was trying to start conservation program by collected the cauda epididymal sperm soon after slaughtered. Two egg yolk-based extenders with different buffers, tris hydroxyl amino methane (TEY20) and trisodium citrate dehydrate (CEY20), were used as comparison to evaluate the post-thawed epididymal sperm quality and fertilizing capacity in artificial insemination program. The results showed that the post-thawed progressive motility of epididymal sperm was 40% and 39.17%, while viability was 65.99% and 63.26% and membrane integrity was 65.43% and 63.03% in TEY20 and CEY20 extenders, respectively. The success rate of pregnancy was 46.67% using post-thawed epididymal sperm in TEY20 and 40% using the one in CEY20 extenders. In conclusion, tris-based and citrate-based extenders have similar ability to maintain the epididymal sperm quality and its fertilizing capacity. Key words: artificial insemination, epididymal sperm, spotted buffalo ABSTRAKKerbau belang merupakan jenis kerbau unik yang ditemukan di Tana Toraja, Sulawesi Selatan. Populasi ternak ini menurun secara drastis karena tingginya laju pemotongan pada upacara adat Rambu Solo', serta keyakinan dalam adat yang tidak memperbolehkan kerbau belang jantan melakukan aktivitas reproduksi. Berdasarkan hal tersebut, studi ini dilakukan sebagai langkah awal proses konservasi dengan menggunakan cauda epididimis sebagai sumber sperma potensial. Studi ini menggunakan dua jenis bufer dalam bahan pengencer berbasis kuning telur, yaitu tris hidroxil amino metana (TEY20) dan trisodium sitrat dehidrat (CEY20), untuk menguji kualitas dan daya fertilitas sperma pasca-thawing dalam proses inseminasi buatan. Hasil yang diperoleh menunjukkan bahwa persentase motilitas progresif adalah sebesar 40% dan 39.17%, dengan persentase hidup mati sebesar 65.99% dan 63.26%, serta persentase membran plasma utuh sebesar 65.43% dan 63.03% dalam bahan pengencer TEY20 dan CEY20 secara berturut-turut. Dapat disimpulkan bahwa bahan pengencer berbasis tris dan sitrat memiliki kemampuan yang sama dalam mempertahankan kualitas dan daya fertilitas sperma epididimis kerbau belang pasca-thawing.

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Section: Resultsmentioning

confidence: 99%

The Success Rate of Artificial Insemination Using Post-Thawed Spotted Buffaloes Epididymal Sperm

Yulnawati¹,

Maheshwari²,

Rizal³

et al. 2013

Med Pet

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“…Initial evaluation and holding of semen was carried out according to Andrabi et al (2008). Semen samples from each bull were divided into three aliquots for dilution.…”

Section: Semen Processingmentioning

confidence: 99%

Effect of equilibration times, freezing, and thawing rates on post-thaw quality of buffalo (Bubalus bubalis) bull spermatozoa

Shah

Andrabi²,

Qureshi

2016

Andrology

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SUMMARYThe effects of equilibration times (E1, 2 h; E2, 4 h; E3, 6 h), freezing rates (FR1, manual, 5 cm above liquid nitrogen (LN 2 ) for 10 min, plunging in LN 2 ; FR2, programmable ultra-fast, holding at +4°C for 2 min, from 4 to À10°C at À10°C/min, from À10 to À20°C at À15°C/min, from À20 to À120°C at À60°C/min, holding at À120°C for 30 sec, plunging in LN 2 ), and thawing rates (T1, 37°C for 30 sec; T2, 50°C for 15 sec; T3, 70°C for 7 sec) were evaluated on quality of buffalo bull spermatozoa. Progressive motility (%), rapid velocity (%), average path velocity (VAP, lm/s), straight line velocity (VSL, lm/s), and mitochondrial transmembrane potential (%) were higher (p < 0.05) with E2, FR2, and T3 compared to other groups. Sperm curved line velocity (VCL, lm/s) was higher (p < 0.05) with E2 and FR2 compared to other groups. Sperm straightness (%) and linearity (LIN, %) were higher (p < 0.05) with E2 compared to other groups. Sperm LIN was affected (p < 0.05) with T3 compared to other groups. Supravital-plasma membrane integrity (%), viability and acrosome integrity (%) of spermatozoa were higher (p < 0.05) with E2 and FR2 compared to other groups. Sperm DNA integrity (%) was higher (p < 0.05) with FR2 and T1 compared to other groups. We concluded that inclusion of 4 h-equilibration time, programmable ultra-fast freezing rate, and rapid thawing at 70°C for 7 sec in cryopreservation protocol improves the post-thaw quality of buffalo bull spermatozoa.

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“…Entretanto, quando utilizada uma concentração mais baixa da gema de ovo (1%), bem como na sua ausência, os resultados do vigor espermático chegaram a zero já a partir do D2, indicando que sua presença em concentrações maiores pode proporcionar uma melhor condição para a manutenção da célula espermática suína sob refrigeração, comprovando sua ação protetora (Tabela 1). A ação protetora da gema de ovo já foi descrita por alguns autores (29,30) que computam esse efeito às lipoproteínas de baixa densidade, que permanecem firmemente ligadas aos espermatozoides, estabilizando a membrana espermática pela neutralização dos componentes deletérios existentes no plasma seminal. A mesma tendência foi observada para a porcentagem de células móveis.…”

Section: Resultsunclassified

Diferentes Concentrações De Gema De Ovo Em Pó Adicionada Ao Diluente Acp-103® Na Conservação Do Sêmen Suíno

et al. 2016

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ResumoMuitas tentativas são feitas para se melhorar a conservação do sêmen suíno, sendo a gema de ovo conhecida por suas propriedades crioprotetoras. Este trabalho teve por objetivo testar diferentes concentrações de gema de ovo em pó (GOP), adicionada ao diluente água de coco em pó (ACP-103 ® ), e verificar qual mantém melhor a viabilidade espermática. Foram diluídos 36 ejaculados em ACP-103 ® , acrescidos de diferentes concentrações de GOP (0%, 1%, 3%, 5% e 7%) e conservados a 17 °C. Diariamente, foram realizadas análises de vigor e motilidade e nos dias 1 (D0), 3 (D2) e 5 (D4) de conservação foram feitas as de vitalidade, morfologia e resistência osmótica. Utilizou-se o teste estatístico de Kruskal-Wallis e de Dunn's para dados não paramétricos e ANOVA e Tukey para os paramétricos. Queda de vigor e motilidade foi observada em todos os tratamentos. Os GOP-3%, GOP-5% e GOP-7% foram os que melhor mantiveram o vigor espermático em D0 (2,4±0,8; 2,5±1,1 e 2,8±0,9, respectivamente), sem diferenças significativas entre si. O mesmo ocorreu para a motilidade (77±15%, 74±23% e 81±16% em D0). Os resultados das análises de vitalidade, morfologia e resistência osmótica não diferiram. Em conclusão, as concentrações entre 5 e 7% de GOP adicionado ao ACP-103 ® permitem sua utilização como diluente eficiente para manter a qualidade espermática. Palavras-chave: água de coco em pó; conservação; gema de ovo; sêmen suíno. AbstractMany attempts have been made to improve the conservation of boar semen. Egg yolk is known to have cryoprotectant properties. This study aimed to test different concentrations of egg yolk added to the coconut milk powder extender (ACP-103 ® ), and verify which one is better to maintain sperm viability. The ejaculated (36) were diluted in ACP-103 ® supplemented with different concentrations of egg yolk (0%, 1%, 3%, 5% and 7%). The conservation occurred at 17 °C, and vigor and motility analysis were carried out daily. On days 1 (D0), 3 (D2) and 5 (D4) the semen was evaluated for

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Duck egg yolk in extender improves the freezability of buffalo bull spermatozoa

Cited by 73 publications

References 17 publications

The Success Rate of Artificial Insemination Using Post-Thawed Spotted Buffaloes Epididymal Sperm

The Success Rate of Artificial Insemination Using Post-Thawed Spotted Buffaloes Epididymal Sperm

Effect of equilibration times, freezing, and thawing rates on post-thaw quality of buffalo (Bubalus bubalis) bull spermatozoa

Diferentes Concentrações De Gema De Ovo Em Pó Adicionada Ao Diluente Acp-103® Na Conservação Do Sêmen Suíno

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