Dynamic nuclear polarization studies of nitroxyl spin probes in agarose gel using Overhauser‐enhanced magnetic resonance imaging

Meenakumari, V.; Utsumi, Hideo; Hyodo, Fuminori; Jawahar, Ajay; Benial, A. Milton Franklin

doi:10.1002/mrc.4626

Cited by 3 publications

(1 citation statement)

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“…The ESR imaging, using nitroxyl probes, can able to provide such information, but OMRI has the advantage of providing coregistered images containing functional as well as anatomical information . Recently, the ESR and OMRI studies were reported that the optimized agent concentration was observed as 2 mM nitroxyl radicals . Many researchers reported the dynamic nuclear polarization studies at 2 mM agent concentration for ESR/OMR phantom imaging .…”

Section: Introductionmentioning

confidence: 99%

Low‐frequency ESR studies on permeable and impermeable deuterated nitroxyl radicals in corn oil solution

Jebaraj

Utsumi

Benial³

2017

Magnetic Reson in Chemistry

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Low-frequency electron spin resonance studies were performed for 2 mM concentration of deuterated permeable and impermeable nitroxyl spin probes, 3-methoxycarbonyl-2,2,5,5-tetramethyl-pyrrolidine-1-oxyl and 3-carboxy-2,2,5,5,-tetramethyl-1-pyrrolidinyloxy in pure water and various concentrations of corn oil solution. The electron spin resonance parameters such as the line width, hyperfine coupling constant, g factor, rotational correlation time, permeability, and partition parameter were estimated. The broadening of line width was observed for nitroxyl radicals in corn oil mixture. The rotational correlation time increases with increasing concentration of corn oil, which indicates the less mobile nature of spin probe in corn oil mixture. The membrane permeability and partition parameter values were estimated as a function of corn oil concentration, which reveals that the nitroxyl radicals permeate equally into the aqueous phase and oil phase at the corn oil concentration of 50%. The electron spin resonance spectra demonstrate the permeable and impermeable nature of nitroxyl spin probes. From these results, the corn oil concentration was optimized as 50% for phantom studies. In this work, the corn oil and pure water mixture phantom models with various viscosities correspond to plasma membrane, and whole blood membrane with different hematocrit levels was studied for monitoring the biological characteristics and their interactions with permeable nitroxyl spin probe. These results will be useful for the development of electron spin resonance and Overhauser-enhanced magnetic resonance imaging modalities in biomedical applications.

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Section: Introductionmentioning

confidence: 99%