Dynamics of Changes in the Gut Microbiota of Healthy Mice Fed with Lactic Acid Bacteria and Bifidobacteria

Abstract

Probiotics are living microorganisms that provide numerous health benefits for their host. Probiotics have various effects on the body; for example, they change gut microbiota, improve the integrity of the epithelial barrier and have anti-inflammatory effects. The use of probiotic supplements that are based on lactic acid bacteria and bifidobacteria is one of the approaches that are used to balance gut microflora. In our study, we evaluated the effects of supplements, which were based on members of the Lactobacillaceae family and bifidobacteria, on the gut microbiome of healthy mice using the 16S rRNA sequencing method. The data that were obtained demonstrated that when mice received the probiotic supplements, statistically significant changes occurred in the composition of the microbiome at the phylum level, which were characterized by an increase in the number of Actinobacteriota, Bacteroidota, Verrucomicrobia and Proteobacteria, all of which have potentially positive effects on health. At the generic level, a decrease in the abundance of members of the Nocardioides, Helicobacter and Mucispirillum genus, which are involved in inflammatory processes, was observed for the group of mice that was fed with lactic acid bacteria. For the group of mice that was fed with bifidobacteria, a decrease was seen in the number of members of the Tyzzerella and Akkermansia genus. The results of our study contribute to the understanding of changes in the gut microbiota of healthy mice under the influence of probiotics. It was shown that probiotics that are based on members of the Lactobacillaceae family have a more positive effect on the gut microbiome than probiotics that are based on bifidobacteria.

Effects of dietary supplementation with dandelion tannins or soybean isoflavones on growth performance, antioxidant function, intestinal morphology, and microbiota composition in Wenchang chickens

“…In addition, there were seven species of bacteria with a significant difference at the family level, according to the LDA effect size analysis. Lactobacillaceae can protect the gut by inhibiting pathogen colonization ( 68 , 69 ) and have a positive effect on the feed efficiency of chickens ( 70 ). Bifidobacteriaceae have shown beneficial effects in an animal, such as promoting nutrient absorption and improving immunity ( 71 ).…”

Sanziguben polysaccharides improve diabetic nephropathy in mice by regulating gut microbiota to inhibit the TLR4/NF-κB/NLRP3 signalling pathway

“…Akkermansia was confirmed to be positively associated with MET treatment for diabetes and with the expression of tight junction proteins and the integrity of the intestinal barrier (Gryaznova et al. 2022 ). Interestingly, Verrucomicrobia and Akkermansia were individually enriched in the SZP group, suggesting that SZP treatment enhanced intestinal barrier function.…”

Ameliorative Effect of Mannuronate Oligosaccharides on Hyperuricemic Mice via Promoting Uric Acid Excretion and Modulating Gut Microbiota

“…The abundance of pathogenic bacteria Bilophila , Tyzzerella , Bryobacter , Comamonas , and Candidatus_Solibacter was significantly downregulated in the MOS group [ 68 , 69 , 70 ]. Tyzzerella was strongly positively associated with the risk of high cardiovascular risk and obesity-related diseases [ 71 , 72 ]. In this study, Tyzzerella was found to present a strong positive correlation with pro-inflammatory factors, which was in agreement with the findings of Grant et al [ 73 ].…”