Microbiology is the study of microbes where one of the materials is bacterial genetics. The results of observations about microbiology learning students experienced difficulties in understanding bacterial genetics, and there was no teaching material on bacterial genetics. Flipbook-based teaching materials are an alternative solution for teaching bacterial genetics well to students. The purpose of this study was to develop flipbook-based bacterial genetics teaching materials in microbiology courses at IKIP Budi Utomo. The development research model used is 4D including define, design, develop, and disseminate. This research is limited to the development stage. The results showed that the assessment of bacterial genetics teaching materials from material experts was 90.38%, media experts were 90%, field practitioner tests were 91.67% so that it met the very worthy criteria. The readability test with most of the students answered strongly agree with the statements about bacterial genetics teaching materials. The flipbook-based bacterial genetics teaching materials in the Microbiology course can improve learning motivation.Keywords: Learning materials, bacterial genetics, flipbook maker