Efek Proteksi Kombinasi Minyak Wijen (Sesame Oil) dengan α-Tocopherol terhadap Steatosis melalui Penghambatan Stres Oksidatif pada Tikus Hiperkolesterolemia

Abstract

AbstrakMinyak wijen (MW) yang banyak mengandung polyunsaturated fatty acid (PUFA) berfungsi menurunkan kadar lipid serum melalui induksi β oksidasi di mitokondria. Proses ini menghasilkan produk sampingan berupa radikal bebas. Vitamin E (α-tocopherol) diketahui menghambat aktifitas radikal bebas. Penelitian ini bertujuan untuk membuktikan efek sinergisme MW dan α-tocopherol dalam menghambat steatosis yang diakibatkan keadaan hiperkolesterol. Hasil penelitian menunjukan bahwa diperoleh perbedaan bermakna (p<0,05) terutama dosis MW 1,2 ml pada semua parameter antara kelompok hiperkolesterol, MW dan MW+α-tocopherol. Kadar kolesterol kelompok hiperkolesterol (140,925±10,5) AbstractSesame oil (MW) contains great numbers of polyunsaturated fatty acid (PUFA) that functioned to decrease the level of serum lipid by induction of β oxidize in mitochondria. This process produce secondary product, i.e. free radical. Vitamin E (α-tocopherol) was known as the inhibitor of free radical activity. This study was aimed to verify the synergism effect of sesame oil and α-tocopherol in inhibiting steatosis which caused by hypercholesterol condition. The results showed that there is significant difference (p<0,05) especially for MW doses of 1,2 ml on all parameters among hypercholesterol groups, MW and MW+α-tocopherol. Cholesterol level of hypercholesterol group is 140.925±10.5 mg/dl, while MW group is 93.845±4.37 mg/dl and MW+α-tocopherol group for 92.90±8.5 mg/dl. Triglyceride level of hypercholesterol groups is 154.78±11.09 mg/dl, MW group for 184.64±3.87 mg/dl, and MW+α-tochopherol group for 66.89 ± 6.03 mg/dl. Level of MDA in MW groups is 0.257±0.013 nmol/mg protein, hypercholesterol group for 0.847±0.036 nmol/mg protein and MW+α-tocopherol group combination is 0.092±0.006 nmol/mg protein.Level of SOD on MW+α-tocopherol treatment was 253.82±16.63) U/mg protein, MW treatment of 208.7±11.27 U/mg protein, and hypercholesterol treatment for 139.7±2.82 U/mg protein. Histology of steatosis was found more in hypercolesterol group along with inflammation, while on combination group of MW+α-tochopherol showed the lowest steatosis in the histology.

Pengaruh pemberian kombinasi minyak rami dengan minyak wijen terhadap kadar kolesterol high density lipoprotein (HDL) pada tikus sprague dawley dislipidemia

“…Penurunan kolesterol juga diakibatkan penurunan aktifitas HMG-CoA reductase oleh sesamin, sedangkan peningkatan ekskresi kolesterol ini juga dikarenakan semakin meningkatnya produksi kolesterol HDL, sehingga terjadi penurunan kolesterol LDL, VLDL dan trigliserida. [43][44][45]…”