AbstrakMinyak wijen (MW) yang banyak mengandung polyunsaturated fatty acid (PUFA) berfungsi menurunkan kadar lipid serum melalui induksi β oksidasi di mitokondria. Proses ini menghasilkan produk sampingan berupa radikal bebas. Vitamin E (α-tocopherol) diketahui menghambat aktifitas radikal bebas. Penelitian ini bertujuan untuk membuktikan efek sinergisme MW dan α-tocopherol dalam menghambat steatosis yang diakibatkan keadaan hiperkolesterol. Hasil penelitian menunjukan bahwa diperoleh perbedaan bermakna (p<0,05) terutama dosis MW 1,2 ml pada semua parameter antara kelompok hiperkolesterol, MW dan MW+α-tocopherol. Kadar kolesterol kelompok hiperkolesterol (140,925±10,5) AbstractSesame oil (MW) contains great numbers of polyunsaturated fatty acid (PUFA) that functioned to decrease the level of serum lipid by induction of β oxidize in mitochondria. This process produce secondary product, i.e. free radical. Vitamin E (α-tocopherol) was known as the inhibitor of free radical activity. This study was aimed to verify the synergism effect of sesame oil and α-tocopherol in inhibiting steatosis which caused by hypercholesterol condition. The results showed that there is significant difference (p<0,05) especially for MW doses of 1,2 ml on all parameters among hypercholesterol groups, MW and MW+α-tocopherol. Cholesterol level of hypercholesterol group is 140.925±10.5 mg/dl, while MW group is 93.845±4.37 mg/dl and MW+α-tocopherol group for 92.90±8.5 mg/dl. Triglyceride level of hypercholesterol groups is 154.78±11.09 mg/dl, MW group for 184.64±3.87 mg/dl, and MW+α-tochopherol group for 66.89 ± 6.03 mg/dl. Level of MDA in MW groups is 0.257±0.013 nmol/mg protein, hypercholesterol group for 0.847±0.036 nmol/mg protein and MW+α-tocopherol group combination is 0.092±0.006 nmol/mg protein.Level of SOD on MW+α-tocopherol treatment was 253.82±16.63) U/mg protein, MW treatment of 208.7±11.27 U/mg protein, and hypercholesterol treatment for 139.7±2.82 U/mg protein. Histology of steatosis was found more in hypercolesterol group along with inflammation, while on combination group of MW+α-tochopherol showed the lowest steatosis in the histology.

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Background: Parkinson disease is characterized with deposition of Lewy Bodies containing αsynuclein happened due to the effect of chronic neuroinflammation that causes the death of dopaminergic neurons through oxidative stress processes, so it involves the response of Nuclear factor erythroid 2-like 2 (Nrf2). Centella asiatica (C.asiatica) contains antioxidant effect, inhibits the aggregation of α-synuclein and improves the locomotor on Parkinson-model animals so it needs to compare to the standard medication. Objective: To compare the C. asiatica extract and Pramipexole to the zebrafish Parkinson model by determining the locomotor activity, α-synuclein expression, and Nrf2. Methods: This study used six groups of zebrafish: negative control, rotenone rotenone [5 μg/L], pramipexole1, 2, 3 (rotenone + pramipexole [3,5] ng/mL, [7] ng/mL, [14] ng/mL), and C. asiatica (rotenone + C. asiatica [10] μg/mL). The observations of locomotor activity of day 0, 14, and 28 were continued to the α-synuclein immunohistochemical examination, and Nrf2 on the midbrain area. Results: There are significant differences in locomotor activity on day 28 among the C. asiatica group with rotenone (p<0,05), while there are no significant differences among the C. asiatica group with pramipexole [7] ng/mL and [14] ng/mL (p>0,05). α-synuclein expression of the C. asiatica group is the lowest and significantly different from all groups (p<0,05), while Nr2 had no significant differences (p>0,05). Conclusion: C. asiatica extract [10] μg/mL is equal to pramipexole [7] ng/mL and [14] ng/mL in improving locomotor activity, but C. asiatica extract holds excellence as it decreases α-synuclein expression better than pramipexole, while Nrf2 expression shows no differences.