Efektivitas Suplementasi Filtrat Jambu Biji dalam Pengencer AirKelapa-Kuning Telur terhadap Kualitas Semen Cair Sapi Bali (THE EFFECTIVENESS OF GUAVA FILTRATE SUPPLEMENTATION IN COCONUT WATER-EGG YOLK DILUTION ON QUALITY OF LIQUID SEMEN OF BALI CATTLE)

Marawali, Aloysius; Abdullah, Muhammad S.; Jalaludin, Jalaludin

doi:10.19087/jveteriner.2019.20.1.20

Cited by 4 publications

(2 citation statements)

References 8 publications

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“…In comparison, the average percentage of acrosome integrity is around 87.5%. This plasma membrane integrity percentage value is almost similar to the average results of study stated by Bebas et al, (2021), which reached around 83.55% but is still lower than the results of study conducted by Marawali et al, (2019), where membrane plasma and acrosome intgerity reached 90.16% and 90.12% respectively. Based on the evaluation of the macroscopic and microscopic characteristics of Bali bull semen, it can be concluded that the collected semen is of good quality and meets the requirements for further processing into frozen semen.…”

Section: Kinematics Of Bali Bull Semen After Freezingsupporting

confidence: 85%

Characteristics and Kinematics of Bali Bull Sperms after Thawing Using Tris Soy Lecithin

Fadilla,

Yusuf,

Toleng

et al. 2023

J. Adv. Zool.

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Soy functions as an extracellular cryoprotectant, which can maintain the integrity of spermatozoa cell membranes with its main content is lecithin. Lecithin from soybeans protects sperm cells from cold stress and reduces the effects of oxidative stress during cryopreservation. This study aimed to analyse the effect of various levels of lecithin diluent on the quality of Bali bull semen during cryopreservation. Semen collection of Bali bull was carried out once a week during four times consecutively using an artificial vagina. The semen was then diluted using the essential ingredient Tris Aminomethan with the addition of powdered soy lecithin; P1 (1 %), P2 (3 %) and P3 (5 %), respectively. Andromed® (K1) as a positive control and Tris without soy lecithin (K2) as a negative control. The parameters observed were motility, progressive motility, kinematics, viability, membrane integrity, and acrosome integrity. The results of this study showed that the dilution of semen with soy lecithin before and after thawing the semen quality was not significantly different (P< 0.05) in motility, viability, plasma membrane integrity, and acrosome integrity. Meanwhile, the kinematics of VAP, VCL, VSL, DAP, DCL, and DSL showed that the average quality increased at P3 compared to K1, K2, P1, and P2, which decreased after thawing or were significantly different (P< 0.05). It can be concluded that Bali bull semen diluted with 3% and 5% of tris soy lecithin produces good characteristics and kinematics, can protect spermatozoa from cold shock.

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Section: Kinematics Of Bali Bull Semen After Freezingsupporting

confidence: 85%

Characteristics and Kinematics of Bali Bull Sperms after Thawing Using Tris Soy Lecithin

Fadilla,

Yusuf,

Toleng

et al. 2023

J. Adv. Zool.

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“…ISSN 2721-1304, ISSN 2721ISSN -1207 Copyright © 2023, Journal La Lifesci, Under the license CC BY-SA 4.0 yolk, honey, and guava filtrate (Sumadiasa, et al, 2015;Astuti, 2018;Malik, et al, 2018;Marawali, et al, 2019). Nevertheless, the standard semen quality produced only lasts for 3-4 days.…”

mentioning

confidence: 99%

Integrity of Sperm Cell Bali Bull Preserved at 5°C Using a Diluent Formulation of Extracted Red Fruit and Coconut Water

Ervandi

2023

JLL

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This study aims to determine the role of the best diluent in protecting the integrity of the spermatozoa membrane after storage using an alternative diluent formulation of red fruit extract (Pandanus Conoideus Lamk) and coconut water. An alternative diluent formulation featuring red fruit extract (Pandanus Conoideus Lamk) and coconut water was utilized. Fresh semen from Bali Bull was evaluated in the Integrated Field Laboratory, Department of Animal Science of Universitas Muhammadiyah Gorontalo. Bali bull sperm of high quality was diluted using a combination diluent containing red fruit extract (Pandanus Conoideus Lamk), green coconut water, and CEP-2 mixed with 10% egg yolk. Technical term abbreviations were explained at their initial use. Spermatozoa membrane integrity was assessed using the hypoosmotic swelling test (HOST), while chlortetracycline (CTC) fluorescent dye was used to evaluate spermatozoa capacitation and acrosome reaction. The randomized group design (RAK) was used in the research, consisting of four conditions and 10 replications. P0 served as the control group, which received CEP-2 plus 10% egg yolk. P1 was administered EBM diluent AK enriched with 15% KT, while P2 received EBM diluent AK plus 15% KT and fructose 1 mg/ml. P3 was given EBM diluent AK plus 15% KT and fructose 2 mg/ml. Analysis of variance was used to examine the resulting data. The findings indicated that CEP-2 plus 10% egg yolk diluent was more effective than the diluent formulations containing red fruit extract and coconut water. Spermatozoa with intact membranes and no capacitation remain high, while those with capacitation and acrosome reaction remain low.

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Potential of Flamboyant Flower (Delonix regia(Boj. ex Hook) Raf) Extract to Maintain Post-Dilution Quality of Aceh Cattle (Bos taurus indicus) Spermatozoa

et al. 2020

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The objective of this research was to investigate the effect of flamboyant flower extract as a natural antioxidant on sperm quality of Aceh cattle after dilution and equilibration. Fresh semen was collected from 2 healthy bull aged 3.5-4 years old. The research took place in BIB Laboratory. The method used was CRD (Completed Randomized Design), with 6 treatments and 5 replications each The treatments (m/v) were 0%, 0.05%, 0.10%, 0.15% and 0.20% of flamboyant flower extracts. The method used was CRD (Completed Randomized Design), with 6 treatments and 5 replications each. The treatments (m/v) were 0%, 0.05%, 0.10%, 0.15% and 0.20% of flamboyant flower extracts. The data obtained were analyzed by analysis of variance (ANOVA). Parameters evaluated in this study were motility, viability, plasma membrane integrity, and abnormality. Results showed that there was an insignificant effect (p>0.05) of flamboyant flower extract on the quality of Aceh cattle spermatozoa. The best percentage of motility and viability, 80.0% and 88.4%, respectively, were found at P3 (0.15% flamboyant flower extract). In can be concluded from the research that the addition of 0.15% (m/v) D. regia flower extract was able to maintain sperm quality of Aceh cattle to be used in the artificial insemination program.

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Efektivitas Suplementasi Filtrat Jambu Biji dalam Pengencer AirKelapa-Kuning Telur terhadap Kualitas Semen Cair Sapi Bali (THE EFFECTIVENESS OF GUAVA FILTRATE SUPPLEMENTATION IN COCONUT WATER-EGG YOLK DILUTION ON QUALITY OF LIQUID SEMEN OF BALI CATTLE)

Cited by 4 publications

References 8 publications

Characteristics and Kinematics of Bali Bull Sperms after Thawing Using Tris Soy Lecithin

Characteristics and Kinematics of Bali Bull Sperms after Thawing Using Tris Soy Lecithin

Integrity of Sperm Cell Bali Bull Preserved at 5°C Using a Diluent Formulation of Extracted Red Fruit and Coconut Water

Potential of Flamboyant Flower (Delonix regia(Boj. ex Hook) Raf) Extract to Maintain Post-Dilution Quality of Aceh Cattle (Bos taurus indicus) Spermatozoa

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