Potential of Flamboyant Flower (<i>Delonix regia</i>(Boj. ex Hook) Raf) Extract to Maintain Post-Dilution Quality of Aceh Cattle (<i>Bos taurus indicus</i>) Spermatozoa

Muhammad, Marlina; Eriani, Kartini; Fithri, Aida; Rusdi, Amalia; Saputra, Hens

doi:10.1051/e3sconf/202015101044

Cited by 2 publications

(3 citation statements)

References 12 publications

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“…Sperm membrane is also vulnerable during equilibration due to the exposure to cold temperatures and the potential generation of superoxide, which can lead to peroxidation and damage to the phospholipid membrane. This makes it highly dependent on the dilution content and integrity of the spermatozoa membrane during the equilibration process (Muhammad et al, 2020).…”

Section: Introductionmentioning

confidence: 99%

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Optimizing Equilibration Protocols through Ubiquinone Supplementation in Goat Frozen Semen Diluent

Hernawati,

Susilowati,

Mulyati

et al. 2024

Interciencia

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The equilibration period is essential for sperm to adjust to the diluent, thereby preventing severe damage during cryopreservation. The elevation of lipid peroxidation during equilibration, indicating potential oxidative stress involvement, underscores the importance of optimizing equilibration protocols to minimize the detrimental effects of free radical production on sperm quality. This study aims to investigate the supplementation effect of adding ubiquinone to semen diluent on the membrane integrity, motility, and survivability of goat spermatozoa following an hour of equilibration. Ubiquinone supplementation was administered at varying doses: Group 2 (B) received 5 mg/dL, Group 3 (C) received 10 mg/dL, Group 4 (D) received 15 mg/dL, Group 5 (E) received 20 mg/dL, and Group 6 (F) received 25 mg/dL. Group A served as the negative control. Following one-way ANOVA examination of the research data, additional Duncan tests were conducted. The study results revealed that ubiquinone addition significantly (p<0.05) affected the percentage of motility, viability, and membrane integrity of Kacang goat spermatozoa after one hour of equilibration. According to statistical analysis, the optimal concentration of ubiquinone in egg yolk skim milk to sustain motility is 12.5 mg/dL. Conversely, the most effective concentration for preserving the viability and membrane integrity of Kacang goat spermatozoa following equilibration is 25 mg/dL. In conclusion, the addition of ubiquinone to the frozen semen diluent maintains the stability of Kacang goat spermatozoa quality after equilibration.

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Section: Introductionmentioning

confidence: 99%

“…The role of nutrient content in the extender used during equilibration cannot be overstated (Muhammad et al, 2020). The availability of essential nutrients in the extender is vital for providing the necessary support for sperm viability and overall functionality during equilibration.…”

Section: Introductionmentioning

confidence: 99%

Optimizing Equilibration Protocols through Ubiquinone Supplementation in Goat Frozen Semen Diluent

Hernawati,

Susilowati,

Mulyati

et al. 2024

Interciencia

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“…Furthermore, it is necessary to find and evaluate semen diluent used to preserve during chilling or cryopreservation [5]. The use of nature dilution has been widely carried out including using the extract tomatoes [6]. Lycopene as a carotenoid found in tomatoes has been considered in preset studies as an antioxidant to prolong life span of bull semen which undergo the chilling and cryopreservation process [7], though did not obtain yet the motility rate or viability in BB cattle.…”

Section: Introductionmentioning

confidence: 99%

Motility and Viability of Spermatozoa of Belgian Blue Crossbreeds with the Addition of Tomato (Solanum lycopersicum) Extract in Egg Yolk Citrate Diluent

Bintara¹,

Panjono²,

Aji³

2022

Advances in Biological Sciences Research

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Crosses between local cattle and pure Belgian Blue cattle often causes birth difficulty because the calf size is too large. In order to avoid this problem, crossbreeding is not conducted with pure Belgian Blue cattle, but with Belgian Blue crossbreeds. In order to support the crossbreeding program between local cattle and Belgian Blue Crossbreeds, it is necessary to study the semen quality of Belgian Blue crossbreeds. This study was aimed to determine the effect of adding tomato (Solanum lycopersicum) extract to egg yolk citrate diluent on the motility of liquid semen and the viability of spermatozoa in Belgian Blue Crossbreed cattle stored at 5 o C. Semen was collected from a 3-year-old Belgian Blue Crossbreed, using an artificial vagina. Semen examination was carried out at the Animal Physiology and Reproduction Laboratory, Universitas Gadjah Mada. The treatments in this study consisted of egg yolk citrate diluent (Control), egg yolk citrate diluent and 4% of tomato extract (P1), egg yolk citrate diluent and 6% of tomato extract (P2), and egg yolk citrate diluent and 8% of tomato extract (P3). Data obtained from microscopic examination were analyzed using analysis of variance based on Completely Randomized Design (CRD). The results showed that the addition of tomato extract could improve maintaining (P<0.05) motility and viability of spermatozoa stored at 5 o C for 96 hours. Semen motility at P0, P1, P2, and P3 were 36.8%, 42.5%, 41.3% and 46.8%, respectively. The viability of spermatozoa at P0, P1, P2, and P3 was 61.3%, 68.5%, 69.3% and 74.3%, respectively. The addition of 8% of tomato extract presented the best results in maintaining motility (46.8%) and viability of spermatozoa (74.3%) after 96 hours. The conclusion of this study was that the addition of 8% of tomato extract into egg yolk citrate diluent could improve the quality of liquid semen, which included motility and viability of spermatozoa.

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Potential of Flamboyant Flower (Delonix regia(Boj. ex Hook) Raf) Extract to Maintain Post-Dilution Quality of Aceh Cattle (Bos taurus indicus) Spermatozoa

Cited by 2 publications

References 12 publications

Optimizing Equilibration Protocols through Ubiquinone Supplementation in Goat Frozen Semen Diluent

Optimizing Equilibration Protocols through Ubiquinone Supplementation in Goat Frozen Semen Diluent

Motility and Viability of Spermatozoa of Belgian Blue Crossbreeds with the Addition of Tomato (Solanum lycopersicum) Extract in Egg Yolk Citrate Diluent

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