Effect of biotic elicitors on the accumulation of bilobalide and ginkgolides in Ginkgo biloba cell cultures

Kang, Shin-Min; Jiang, Min; Kim, Yong-Duck; Karigar, Chandrakant S.; Kim, Seon-Won; Goo, Gwan-Hyo; Choi, Myung-Suk

doi:10.1016/j.jbiotec.2008.09.007

Cited by 65 publications

(28 citation statements)

References 13 publications

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“…In the other hand, elicitation is very attractive strategy for increasing the metabolite productivity in in vitro culture system. However, addition of elicitor decreased the cell growth due to medium acidification (Kang et al 2006), moderate browning or rupture of cell membrane (Kang et al 2009) caused by cell-to-cell contact. In this experiment, immobilization of cell culture using jute fiber was conducted to repair cell-to-cell contact and increase the cell growth before elicitation.…”

Section: The Effect Of Mj Sa and Mj + Sa In Enhancing Bilobalide Anmentioning

confidence: 99%

Effect of methyl jasmonate and salycilic acid synergism on enhancement of bilobalide and ginkgolide production by immobilized cell cultures of Ginkgo biloba

Sukito¹,

Tachibana

2016

Bioresour. Bioprocess.

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Background: Bilobalide and ginkgolides are reported to be present only in Ginkgo biloba. However, only trace amounts of bilobalide and ginkgolides are contained in the ginkgo leaves. Nowadays, there has been considerable interest in plant cell cultures as a potential alternative to traditional agriculture for the industrial production of secondary metabolites. Much effort has been put into the use of in vitro cultures as one attractive biotechnological strategy for producing bilobalide and ginkgolides of commercial interest. The aim of this study was to enhance the production of bilobalide and ginkgolides A, B, and C in cell cultures of G. biloba using immobilized cell cultures and the process of elicitation.Results: Based on the effect of the immobilization components on the free cell suspension cultures, it was considered that the jute fiber acted as an elicitor and forced the cells to release the product into the culture media. The resulting biomass was approximately 1.4 times higher than in the cell suspension cultures, and the production of bilobalide and ginkgolides A, B, and C increased 5.0, 3.3, 6.1, and 4.1 times, respectively. Eliciting with methyl jasmonate (MJ) and salicylic acid (SA) in the immobilized cells enhanced bilobalide and ginkgolides A, B, and C, compared with the unelicited controls. The highest accumulation was observed using a combination of 0.1 mM MJ + 0.1 mM SA in the immobilized cells, which produced 1.78, 1.95, 2.05, and 2.95 times more bilobalide and ginkgolides A, B, and C, respectively, than the controls. Conclusions:The positive effects of immobilized cell cultures using jute fiber and the synergism effect of SA and MJ on immobilized cells of G. biloba appear to be the optimal conditions for continuous in vitro production for commercial purposes. This is the first report on analyzing the effects of jute fiber as immobilized cell material on G. biloba cell cultures and the synergism of MJ and SA on bilobalide and ginkgolide production.

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Section: The Effect Of Mj Sa and Mj + Sa In Enhancing Bilobalide Anmentioning

confidence: 99%

Effect of methyl jasmonate and salycilic acid synergism on enhancement of bilobalide and ginkgolide production by immobilized cell cultures of Ginkgo biloba

Sukito¹,

Tachibana

2016

Bioresour. Bioprocess.

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“…To produce high quality and quantities of secondary compounds of commercial interest, many methods have been developed. The elicitation strategy is one of the most important methods for production of flavonol glycosides and ginkgolides (Hao et al 2010;Kang et al 2009). Use of this method reduces the time to attain a high yield of product and increases the yield of secondary compounds (Kang et al 2006;Guo et al 2011;Zhao et al 2013).…”

Section: Introductionmentioning

confidence: 99%

“…However, the existing literature in Ginkgo cell culture mainly focuses on the yield of secondary metabolites. Kang et al (2006Kang et al ( , 2009Kang et al ( , 2010 studied the production of bilobalide and ginkgolides using methyl jasmonate (MJ), salicylic acid (SA), bacteria and KCl as elicitors. Hao et al (2009Hao et al ( , 2010 investigated flavonoid accumulation induced by fungal endophytes and UV-B irradiation.…”

Section: Introductionmentioning

confidence: 99%

Effect of varying NaCl doses on flavonoid production in suspension cells of Ginkgo biloba: relationship to chlorophyll fluorescence, ion homeostasis, antioxidant system and ultrastructure

et al. 2014

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Ginkgo suspension cells were used to investigate the mechanism that governs the shift between primary and secondary metabolism under NaCl elicitation. The production of three flavonol glycosides, chlorophyll fluorescence, ion content, the antioxidant system, and the cellular ultrastructure in the presence of NaCl doses from 5 to 175 mM were examined. At low salt doses (5-50 mM), cell growth and flavonol glycosides accumulation were stimulated without damaging cell structure or inducing oxidative stress by maintaining high K ? and chlorophyll content. At moderate salt doses (75-125 mM), the cells could withstand the salt stress without an impact on survival by changing internal cellular structure, maintaining high levels of K ? and Ca 2? and increasing anti-oxidative enzyme activities rather than flavonol glycosides to counteract the inhibition of the photosystem II, the accumula

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“…Cell cultures of G. biloba with biotic elicitors Candida albicans resulted in enhanced accumulation of bilobalide and ginkgolide as compared to control. Bilobalide content was significantly increased (30.5 mg/gm) as compared to control 7.0 mg/gm after 48 h of treatment (Kang et al, 2009 (He et al, 2009). They also found decreased phenolic content in Ginkgo leaves.…”

Section: Role Of Ginkgo Biloba Metabolite Upon Different Stress Condimentioning

confidence: 85%

Advances in Ginkgo biloba research: Genomics and metabolomics perspectives

Mohanta¹

2012

Afr. J. Biotechnol.

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The maiden hair tree, Ginkgo biloba is very much resistant to a wide spectrum of biotic and abiotic stress conditions. It hardly seems to be attacked by any herbivore or microbe. In spite of its strong resistant nature to wide stress conditions, only little research has been carried out at genomics and metabolomics level to understand its mechanism of stress resistance. Ginkgolide and bilobalide, the unique terpene trilactone present in G. biloba were found to play an important role during stress conditions. Massive parallel sequencing of G. biloba leads to generation of 64057 expressed sequence tags (EST's). Beside this, chloroplast genome (cpDNA) of G. biloba has been sequenced. This massive parallel sequencing of ESTs, along with cpDNA genome, will open the door for high throughput genomic research.

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Effect of biotic elicitors on the accumulation of bilobalide and ginkgolides in Ginkgo biloba cell cultures

Cited by 65 publications

References 13 publications

Effect of methyl jasmonate and salycilic acid synergism on enhancement of bilobalide and ginkgolide production by immobilized cell cultures of Ginkgo biloba

Effect of methyl jasmonate and salycilic acid synergism on enhancement of bilobalide and ginkgolide production by immobilized cell cultures of Ginkgo biloba

Effect of varying NaCl doses on flavonoid production in suspension cells of Ginkgo biloba: relationship to chlorophyll fluorescence, ion homeostasis, antioxidant system and ultrastructure

Advances in Ginkgo biloba research: Genomics and metabolomics perspectives

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