Effect of combining two genes for partial resistance to <i>Barley yellow dwarf virus‐</i>PAV (BYDV‐PAV) derived from <i>Thinopyrum intermedium</i> in wheat

Jahier, Joseph; Chain, Florian; Barloy, Dominique; Tanguy, Anne-Marie; Lemoine, Jocelyne; Riault, G.; Margalé, E.; Trottet, Maxime; Jacquot, Emmanuel

doi:10.1111/j.1365-3059.2009.02084.x

Cited by 15 publications

(10 citation statements)

References 34 publications

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“…1, 2). Similar observations were made by Jahier et al (2009) in wheat by combining Bdv2 and the group-2 chromosome arm carrying BYDV resistance derived from Thinopyrum intermedium. In their studies, they also found a very low virus titre and a signiWcantly lower number of infected plants in those lines carrying both genes compared to the single loci.…”

Section: Discussionsupporting

confidence: 77%

Pyramiding of Ryd2 and Ryd3 conferring tolerance to a German isolate of Barley yellow dwarf virus-PAV (BYDV-PAV-ASL-1) leads to quantitative resistance against this isolate

et al. 2011

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Barley yellow dwarf virus (BYDV) is an economically important pathogen of barley, which may become even more important due to global warming. In barley, several loci conferring tolerance to BYDV-PAV-ASL-1 are known, e.g. Ryd2, Ryd3 and a quantitative trait locus (QTL) on chromosome 2H. The aim of the present study was to get information whether the level of tolerance against this isolate of BYDV in barley can be improved by combining these loci. Therefore, a winter and a spring barley population of doubled haploid (DH) lines were genotyped by molecular markers for the presence of the susceptibility or the resistance encoding allele at respective loci (Ryd2, Ryd3, QTL on chromosome 2H) and were tested for their level of BYDV-tolerance after inoculation with viruliferous (BYDV-PAV-ASL-1) aphids in Weld trials. In DH-lines carrying the combination Ryd2 and Ryd3, a signiWcant reduction of the virus titre was detected as compared to lines carrying only one of these genes. Furthermore, spring barley DH-lines with this allele combination also showed a signiWcantly higher relative grain yield as compared to lines carrying only Ryd2 or Ryd3. The QTL on chromosome 2H had only a small eVect on the level of tolerance in those lines carrying only Ryd2, or Ryd3 or a combination of both, but the eVect in comparison to lines carrying no tolerance allele was signiWcant. Overall, these results show that the combination of Ryd2 and Ryd3 leads to quantitative resistance against BYDV-PAV instead of tolerance.

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Section: Discussionsupporting

confidence: 77%

Pyramiding of Ryd2 and Ryd3 conferring tolerance to a German isolate of Barley yellow dwarf virus-PAV (BYDV-PAV-ASL-1) leads to quantitative resistance against this isolate

et al. 2011

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“…However, most focused on pathogen control over short periods of time (one or a few crop seasons or a limited number of pathogen generations) and did not take into account that the pathogen population might evolve (e.g., Brown et al, 1996; Porter et al, 2000; Maqbool et al, 2001; Jahier et al, 2009; Tan et al, 2010; Brunner et al, 2012; Obala et al, 2012; Zeller et al, 2012; Cingel et al, 2014; McCarville et al, 2014; Rule et al, 2014; Vu et al, 2014; Fukuoka et al, 2015). These comparative studies did not actually compare the durability of resistance genes.…”

Section: Comparisons Of Strategies For the Short-term Control Of Pathmentioning

confidence: 99%

Combining Selective Pressures to Enhance the Durability of Disease Resistance Genes

Delmotte¹,

Bourguet²,

Franck

et al. 2016

Front. Plant Sci.

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The efficacy of disease resistance genes in plants decreases over time because of the selection of virulent pathogen genotypes. A key goal of crop protection programs is to increase the durability of the resistance conferred by these genes. The spatial and temporal deployment of plant disease resistance genes is considered to be a major factor determining their durability. In the literature, four principal strategies combining resistance genes over time and space have been considered to delay the evolution of virulent pathogen genotypes. We reviewed this literature with the aim of determining which deployment strategy results in the greatest durability of resistance genes. Although theoretical and empirical studies comparing deployment strategies of more than one resistance gene are very scarce, they suggest that the overall durability of disease resistance genes can be increased by combining their presence in the same plant (pyramiding). Retrospective analyses of field monitoring data also suggest that the pyramiding of disease resistance genes within a plant is the most durable strategy. By extension, we suggest that the combination of disease resistance genes with other practices for pathogen control (pesticides, farming practices) may be a relevant management strategy to slow down the evolution of virulent pathogen genotypes.

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“…Likewise, inferences about genomic similarities amongst closely related or related species can be performed with the application of GISH, thereby making evolutionary and phylogenetic studies possible (Lim et al, 2000b). The application of GISH in plant breeding programs has made the study of chromosomal introgression possible once it exhibits, for example, the karyotype formation of backcrossed hybrids (Jahier et al, 2009).…”

Section: Introductionmentioning

confidence: 99%

Establishment of the genomic in situ hybridization (GISH) technique for analysis in interspecific hybrids of Passiflora

Melo¹,

Silva²,

Souza³

2015

Genet. Mol. Res.

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ABSTRACT. The genomic in situ hybridization (GISH) technique was applied to Passiflora interspecific F 1 HD13-133 hybrids (Passiflora sublanceolata x Passiflora foetida) and HD15-101 (Passiflora gardineri x Passiflora gibertii), and the backcrossed hybrids (BC 1 ) HD18-106 and HD18-113 (Passiflora sublanceolata x HD13-133). GISH was performed using genomic probes prepared with the DNA from the paternal genitor, whereas the maternal DNA was used as blocking DNA and employed at various concentrations (20X, 40X, 60X, and 100X) in relation to the probe concentration. At the same time, GISH was applied with the use of simultaneous probes from both genomes, paternal and maternal, that were detected with avidin-FITC and antidigoxigenin-rhodamine, respectively. Both methodologies allowed the distinguishing of the maternal and paternal genomes, thus confirming the hybrid nature of all the analyzed genotypes. Furthermore, the presence of recombinant chromosomes in BC 1 hybrids revealed the occurrence of meiotic recombination in HD13 hybrids. This application 2177 ©FUNPEC-RP www.funpecrp.com.br Genetics and Molecular Research 14 (1): 2176-2188 (2015 GISH technique for Passiflora of the GISH technique is an important step towards genomic analyses of Passiflora hybrids: it can broaden the phylogenetic and evolutionary studies of the genus and, at the same time, contribute to breeding programs.

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Effect of combining two genes for partial resistance to Barley yellow dwarf virus‐PAV (BYDV‐PAV) derived from Thinopyrum intermedium in wheat

Cited by 15 publications

References 34 publications

Pyramiding of Ryd2 and Ryd3 conferring tolerance to a German isolate of Barley yellow dwarf virus-PAV (BYDV-PAV-ASL-1) leads to quantitative resistance against this isolate

Pyramiding of Ryd2 and Ryd3 conferring tolerance to a German isolate of Barley yellow dwarf virus-PAV (BYDV-PAV-ASL-1) leads to quantitative resistance against this isolate

Combining Selective Pressures to Enhance the Durability of Disease Resistance Genes

Establishment of the genomic in situ hybridization (GISH) technique for analysis in interspecific hybrids of Passiflora

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