Effect of extraction techniques on properties of polysaccharides from Enteromorpha prolifera and their applicability in iron chelation

Chi, Yongzhou; Li, Yinping; Zhang, Gaoli; Gao, Yaqi; Ye, Han; Gao, Jian; Wang, Peng

doi:10.1016/j.carbpol.2017.11.104

Cited by 116 publications

(62 citation statements)

References 45 publications

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“…When increasing the temperature from 70 °C to 90 °C, the iron contents in the galactomannan–iron(III) complex decreased in a similar fashion. This temperature-based phenomenon was also reported in the work of Chi et al [12], who prepared similar complexes using polysaccharides from a different biological source. In the reported work, the iron content of the polysaccharide–iron(III) complexes obtained from Enteromorpha prolifera decreased when the synthesis temperature increased above 50 °C.…”

Section: Resultssupporting

confidence: 79%

“…According to a recent report, it was found that polysaccharides from Astragalus membranaceus [5], Enteromorpha prolifera [12], and the mushroom Inonotus obliquus [2] are potential candidates for preparing less biologically-hazardous polysaccharide–iron(III) complexes. These newly investigated complexes have demonstrated additional beneficial bioactivities beyond those of iron, including anti-tumor and anti-oxidation activity.…”

Section: Introductionmentioning

confidence: 99%

“…It was speculated that these additional benefits are derived from the original biological activities of the component polysaccharides. Regarding polysaccharide–iron(III) complexes, the polysaccharide acts as the ligand and non-specifically interacts with the Fe 3+ (electron acceptor) to form polysaccharide–iron(III) complexes that can be administered as organic iron supplements [11,12]. Recently, it was found that the galactomannan from Sesbania seed has been identified as a non-noxious and bioactive polysaccharide for living organisms [13,14].…”

Section: Introductionmentioning

confidence: 99%

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Synthesis and Characterization of an Antioxidative Galactomannan–Iron(III) Complex from Sesbania Seed

Huang¹,

Tao²,

Li³

et al. 2018

Polymers

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Galactomannan, a water-soluble polymer in the cell wall of leguminous plants, has been proven to possess anticancer and antioxidative activity. In this work, galactomannan with different molecular weights (GM-40 and GM-65) was obtained from Sesbania seeds and synthesized into galactomannan–iron(III) complexes, which are termed as GM-40-Fe and GM-65-Fe, respectively. These galactomannan–iron(III) complexes are intended to function as organic iron supplements to treat iron deficiency with the added benefit of antioxidative activity. The prepared galactomannan–iron(III) complexes were characterized for chemical composition, morphology, antioxidant capacity, and bioavailability in vitro. The results showed that galactomannan–iron(III) complexes could be produced with iron contents as high as 65.4 mg/g. Antioxidant assays indicated that both GM-40-Fe and GM-65-Fe exhibited antioxidant activities for scavenging radicals in vitro. The iron release/bioavailability assays showed that the iron was easily released into artificial gastric and intestinal juices, resulting in iron release rates of 88–94% over 300 min. These results suggest that galactomannan–iron(III) complexes synthesized from Sesbania seed polysaccharides are capable of being administered as organic iron supplements to patients with iron deficiency.

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Section: Resultssupporting

confidence: 79%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Synthesis and Characterization of an Antioxidative Galactomannan–Iron(III) Complex from Sesbania Seed

Huang¹,

Tao²,

Li³

et al. 2018

Polymers

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“…Polysaccharides commonly exist in various animals, plants, fungus, and algae as polymeric carbohydrate molecule and have exhibited anticoagulant, antioxidant, antitumor, and immunoregulatory activities [9–11] with relatively low toxicity [12]. As is known to us, the properties and bioactivities of polysaccharides were directly associated with their chemical characteristics [13], which could be influenced by extraction techniques [14, 15]. Hot water extraction has been commonly employed to extract crude polysaccharides due to the simple process and low cost [16, 17].…”

Section: Introductionmentioning

confidence: 99%

Structural Characterization and Antitumor Activity of Polysaccharides from Kaempferia galanga L.

Yang

Feng

et al. 2018

Oxidative Medicine and Cellular Longevity

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The water-soluble polysaccharides from Kaempferia galanga L. (KGPs) were extracted and purified, and their structural characteristics and antitumor activity were further investigated. The UV spectrum, high-performance gel permeation chromatography (HPGPC), Fourier-transform infrared spectroscopy (FTIR), and ion chromatography (IC) were employed to evaluate the structural characteristics, and H22 tumor-bearing mice model was established to demonstrate the antitumor activity. Physicochemical analysis and UV spectrum results showed that the proportions of total sugar, protein, and uronic acid in KGPs were 85.23%, 0.54%, and 24.17%, respectively. HPGPC, FTIR, and IC indicated that KGPs were acidic polysaccharides with skeletal modes of pyranose rings and mainly composed of arabinose and galactose with the average molecular weight of 8.5 × 105 Da. The in vivo antitumor experiments showed that KGPs could effectively protect the thymus and spleen of tumor-bearing mice from solid tumors and enhance the immunoregulatory ability of CD4+ T cells, the cytotoxic effects of CD8+ T cells and NK cells, and finally resulting in the inhibitory effects on H22 solid tumors. This study provided a theoretical foundation for the practical application of KGPs in food and medical industries.

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“…Maca polysaccharide (LMP) can be prepared by solvent extraction, acid extraction, and alkaline extraction methods (Zha, Zhao, Chen, et al, 2014b). The extraction processes have inevitable correlations with the quality level of the extracts (Chi et al, 2018, Kim & Iwahashi, 2015, Skenderidis, Petrotos, Giavasis, Hadjichristodoulou, & Tsakalof, 2017. Therefore, efficient and controllable extraction processes not only can guarantee the quality of the Maca polysaccharide, but also play the role of enhancing and promoting the biological activities of Maca polysaccharide.…”

mentioning

confidence: 99%

Optimization of Maca polysaccharide extraction process and its chemo‐protective effects on cyclophosphamide‐induced mice

Wang

Huang

et al. 2018

J Food Process Engineering

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In this study, the extraction process of Maca polysaccharide (LMP) was deeply studied and its chemo‐protective effects on Cyclophosphamide (Cy)‐induced mice were evaluated. By single factor investigation and response surface methodology optimization, it was found that the liquid‐to‐solid ratio, extraction time, and extraction temperature were the main factors which affected the extraction yield of LMP. The optimum extraction process was obtained by Box–Behnken design combined with response surface methodology. It was found that, at the condition of liquid‐to‐solid ratio of 30:1; extraction time of 2.3 hr; and extraction temperature of 90°C, the yield of the LMP could reach 46.56%. Furthermore, by oral administration of LMP, the cyclophosphamide‐induced mice showed the proliferation of lymphocyte and the phagocytic capacity of macrophages’ enhancements. The number of white blood cell, red blood cell, and platelets in the peripheral blood were up‐regulated; the spleen total superoxide dismutase, Catalase, and Glutathione‐peroxidase activities were enhanced, while the Serum malondialdehyde and Triacylglycerols content was reduced. Therefore, our work provided an effective way for extraction of LMP and demonstrated the chemo‐protective effects of LMP on the animal level. Practical application Natural polysaccharides from functional food sources are widely accepted for their bioactive activities. As extraction conditions significantly affected the quality and yield of polysaccharides, the effective extraction process for polysaccharides should be systematically investigated. In the current work, the optimum conditions for polysaccharides extraction from Maca (Lepidium meyenii Walp.) (LMP) were obtained using liquid‐to‐solid ratio of 30:1 for 2.3 hr at 90°C. The Mass characterization and Monosaccharide Composition of LMP were also determined. Furthermore, the extracted LMP exhibited well Chemo‐protective activities on Cyclophosphamide‐induced mice. Therefore, our work showed the potential applications of Maca polysaccharides as the easily accessible source of natural immunopotentiating agents in function food and pharmaceutical industry.

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Effect of extraction techniques on properties of polysaccharides from Enteromorpha prolifera and their applicability in iron chelation

Cited by 116 publications

References 45 publications

Synthesis and Characterization of an Antioxidative Galactomannan–Iron(III) Complex from Sesbania Seed

Synthesis and Characterization of an Antioxidative Galactomannan–Iron(III) Complex from Sesbania Seed

Structural Characterization and Antitumor Activity of Polysaccharides from Kaempferia galanga L.

Optimization of Maca polysaccharide extraction process and its chemo‐protective effects on cyclophosphamide‐induced mice

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