Effect of glycated albumin and cranberry components on interleukin‐6 and matrix metalloproteinase‐3 production by human gingival fibroblasts

Abstract

G-HSA ± LPS may have differential effects on IL-6 and MMP-3 production by human gingival fibroblasts, but both are inhibited by NDM. The study suggests that cranberry phenols may be useful in regulating the host response and perhaps treating periodontitis in patients with poorly controlled diabetes.

Vitamin D attenuates human gingival fibroblast inflammatory cytokine production following advanced glycation end product interaction with receptors for AGE

“…However, HSA did not significantly stimulate IL‐6 or IL‐8 production (except at the highest concentrations tested and at the longest exposure time). This is consistent with our previous work, and others have shown that G‐HSA stimulated IL‐8 and/or IL‐6 production in human chondrocytes and fibroblast‐like synovial cells . Glycated HSA and/or glycated bovine serum albumin (BSA) also stimulated IL‐6 and IL‐8 produced by gingival fibroblasts and monocyte/macrophages .…”

“…Endotoxin levels in G‐HSA and HSA were measured at 13 ± 0.5 and 8.3 ± 1 pg/mL, respectively (Table ). In our previous studies and others, 1 μg/mL Escherichia coli or P. gingivalis LPS (10 6 ‐fold greater than levels detected in these preparations) was needed to stimulate IL‐6 production by human gingival fibroblasts.…”

“…In initial experiments, the cells were exposed for 1, 3, or 6 days to serum‐free DMEM with or without G‐HSA or HSA (50‐125 μg/mL). These concentrations were found to be non‐toxic to this cell line in previous work . In later experiments, cells were exposed to combinations of G‐HSA or HSA and IL‐1β (0.01 nmol/L), IL‐17 (100 ng/mL) or (IL‐1β + IL‐17).…”

“…An HGF cell line derived from a healthy patient with non‐inflamed gingiva was used in this study. The protocol used to establish it and its identification as fibroblastic are described in our earlier work . The cells were maintained in Dulbecco's modified Eagle medium (DMEM; Life Technologies, Grand Island, NY) containing 10% (v/v) newborn calf serum (NCS; Life Technologies) and gentamicin (100 μg/mL; Sigma‐Aldrich, St. Louis, MO) (growth medium), at 37°C in a humidified atmosphere of 5% CO 2 in air.…”

“…Several proteins bind AGEs, including the receptor for AGEs (RAGE) expressed on HGFs and many other cells . Because AGE‐RAGE interaction may upregulate oxidative damage, inflammatory cytokines, and tissue‐destructive matrix metalloproteinases (MMPs), much effort has been placed on blocking RAGE to attenuate inflammation and periodontal destruction. Indeed, blockage of RAGE in diabetic mice diminished bone loss and decreased cytokine and MMP production …”