Effect of Green Tea Polyphenol Epigallocatechin-3-gallate on the Aggregation of αA(66-80) Peptide, a Major Fragment of αA-crystallin Involved in Cataract Development

Kumar, Vijay; Gour, Shalini; Peter, Ocan Simon; Gandhi, Shraddha; Goyal, Pankaj; Pandey, Janmejay; Harsolia, Ram Swaroop; Yadav, Jay Kant

doi:10.1080/02713683.2017.1324628

Cited by 12 publications

(11 citation statements)

References 52 publications

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“…A variety of small molecules are known to inhibit fibrillation by affecting β-amyloid oligomerization, fibrillation or both (Necula et al, 2007;Porat et al, 2006). Fibrillation of αA 66−80 peptide itself was inhibited by epigallocatechin-3-gallate (Kumar et al, 2017) and fibrillation of HU γD-crystallin and bovine α-crystallin under denaturing conditions was blocked by certain dyes (Sharma and Ghosh, 2017), as well as by carnosine (Attanasio et al, 2009), respectively. In addition to reducing protein disulfide, DTT is known to interact with domains of proteins that lack cysteine residues (Alliegro, 2000), and thus the molecule may have been able to interfere with fibrillation via hydrogen bonding to the GP αAcrystallins.…”

Section: Discussionmentioning

confidence: 99%

RETRACTED: Peptide-induced formation of protein aggregates and amyloid fibrils in human and guinea pig αA-crystallins under physiological conditions of temperature and pH

Anbarasu

Jeyarajan

Cheon

et al. 2019

Experimental Eye Research

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αA 66−80 peptide forms with age in the lens nucleus of humans and guinea pigs, and binds to αAcrystallin by hydrophobic bonds to produce protein aggregates. The purpose of the present study was to use negative-stain transmission electron microscopy (TEM) to investigate peptide/crystallin structures that form when αA 66−80 peptide is incubated with recombinant human (HU) and guinea pig (GP) αA-crystallin, and GP αA ins -crystallin, under physiological conditions of temperature and pH. HU and GP αA-crystallin each consists of 173 amino acid residues with only eight differences. GP αA ins -crystallin contains an insertion of 23 amino acids into the sequence for normal GP αA-crystallin. Synthetic αA 66−80 peptide was incubated with each crystallin for 24 hrs in phosphate buffered saline at 37 o C, pH 7.4. Samples were loaded onto electron microscopy grids and analyzed by TEM. With GP αA-crystallin, the αA 66−80 peptide appeared to first form up to 700 nm long, independent peptide fibrils, which subsequently bound numerous αAcrystallin oligomers along the entire length of the fibril to form up to 30 nm thick peptide/oligomer fibril structures. In contrast, HU αA-crystallin somehow prevented independent αA 66−80 peptide fibrillation, and instead joined with the peptides to form amorphous aggregates and linear chains of αA oligomers. When incubated alone, GP αA ins -crystallin eventually lost all oligomeric

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Section: Discussionmentioning

confidence: 99%

RETRACTED: Peptide-induced formation of protein aggregates and amyloid fibrils in human and guinea pig αA-crystallins under physiological conditions of temperature and pH

Anbarasu

Jeyarajan

Cheon

et al. 2019

Experimental Eye Research

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“…Moreover, a polyphenol derived from green tea, epigallocatechin-3-gallate (EGCG) attenuated and reversed the aggregation of αA (66)(67)(68)(69)(70)(71)(72)(73)(74)(75)(76)(77)(78)(79)(80), a peptide fragment derived from αA-crystallin peptide. Since αA (66)(67)(68)(69)(70)(71)(72)(73)(74)(75)(76)(77)(78)(79)(80) aggregates play a role in the destabilization of native crystallin structures, EGCG can potentially prevent onset and reverse cataract formation [122]. In corroboration, green tea (Camellia sinensis) leaf extract mitigated Na selenite-induced reduction on SOD, GSH-Px and CAT activities, and increase in lipid peroxidation in Wistar rat lenses, ex vivo [123].…”

Section: Plant-derived Compounds and Herbal Extractsmentioning

confidence: 89%

Current Trends in the Pharmacotherapy of Cataracts

et al. 2020

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Cataracts, one of the leading causes of preventable blindness worldwide, refers to lens degradation that is characterized by clouding, with consequent blurry vision. As life expectancies improve, the number of people affected with cataracts is predicted to increase worldwide, especially in low-income nations with limited access to surgery. Although cataract surgery is considered safe, it is associated with some complications such as retinal detachment, warranting a search for cheap, pharmacological alternatives to the management of this ocular disease. The lens is richly endowed with a complex system of non-enzymatic and enzymatic antioxidants which scavenge reactive oxygen species to preserve lens proteins. Depletion and/or failure in this primary antioxidant defense system contributes to the damage observed in lenticular molecules and their repair mechanisms, ultimately causing cataracts. Several attempts have been made to counteract experimentally induced cataract using in vitro, ex vivo, and in vivo techniques. The majority of the anti-cataract compounds tested, including plant extracts and naturally-occurring compounds, lies in their antioxidant and/or free radical scavenging and/or anti-inflammatory propensity. In addition to providing an overview of the pathophysiology of cataracts, this review focuses on the role of various categories of natural and synthetic compounds on experimentally-induced cataracts.

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“…Moreover, a polyphenol derived from green tea, epigallocatechin-3-gallate (EGCG) attenuated and reversed the aggregation of αA(66-80), a peptide fragment derived from αA-crystallin peptide. Since αA(66-80) aggregates play a role in the destabilization of native crystallin structures, EGCG can potentially prevent onset and reverse cataract formation [122]. In corroboration, green tea (Camellia sinensis) leaf extract mitigated Na selenite-induced reduction on SOD, GSH-Px and CAT activities, and increase in lipid peroxidation in Wistar rat lenses, ex vivo [123].…”

Section: Plant-derived Compounds and Herbal Extractsmentioning

confidence: 89%

“…Human γcrystallin EGCG attenuated and reversed peroxide-induced aggregation of αA(66-80), a peptide fragment derived from αA-crystallin peptide [122] Green tea (Camellia sinensis) leaves extract Sodium selenite (100 µM)…”

Section: Wistar Rat Lensesmentioning

confidence: 99%

Current Trends in the Pharmacotherapy of Cataracts

Heruye¹,

Nkenyi²,

Singh³

et al. 2020

Updates in Pharmacology

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Cataracts, one of the leading causes of preventable blindness worldwide, refers to lens degradation that is characterized by clouding, with consequent blurry vision. As life expectancies www.videleaf.com antioxidant and/or free radical scavenging and/or antiinflammatory propensity. In addition to providing an overview of the pathophysiology of cataracts, this review focuses on the role of various categories of natural and synthetic compounds on experimentally-induced cataracts.

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Effect of Green Tea Polyphenol Epigallocatechin-3-gallate on the Aggregation of αA(66-80) Peptide, a Major Fragment of αA-crystallin Involved in Cataract Development

Cited by 12 publications

References 52 publications

RETRACTED: Peptide-induced formation of protein aggregates and amyloid fibrils in human and guinea pig αA-crystallins under physiological conditions of temperature and pH

RETRACTED: Peptide-induced formation of protein aggregates and amyloid fibrils in human and guinea pig αA-crystallins under physiological conditions of temperature and pH

Current Trends in the Pharmacotherapy of Cataracts

Current Trends in the Pharmacotherapy of Cataracts

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