Effect of hexachlorocyclohexane feeding on testicular tissue of pure inbred Swiss mice

Sk, Nigam; Bc, Lakkad; Ab, Karnik; Kn, Thakore; Dk, Bhatt; Ka, Babu; Sk, Kashyap

doi:10.1007/bf01769983

Cited by 16 publications

(4 citation statements)

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“…A large number of reports revealed that the infertility and varying degrees of testicular dysfunctions in men (Whorton et al -1977) and experimental animals (Keplinger et al -1968, Harris et al -1974 have resulted from exposure to different pesticides or insecticides. It has also been reported that different organochlorine and organophosphate compounds produced detrimental changes in the seminiferous epithelium of experimental animals (Datta and Dikshth -1973;Nigam et al -1979) although the histological appearance of the Leydig cells and Sertoli cells remained unaltered (Potashnik et al -1978). Recently Shivanandappa and Krishnakumari (1983) histochemically revealed an inhibition in testicular 3P-HSD and 170-HSD activities following long-term feeding of hexachlorocyclohexane.…”

Section: Introductionmentioning

confidence: 97%

Effect of Quinalphos on Testicular Steroidogenesis in Rats

et al. 2009

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The effect of quinalphos (250 pg/kg i.p.) an organophosphorus insecticide treatment for 13 and 26days on the testicular steroidogenic enzymes viz. 30-Hydroxysteroid Dehydrogenase and 170-Hydroxysteroid Dehydrogenase, as well as cholesterol content and histology of the testes of the Wistar strain rats was studied. The time duration of 13 days is approximately equivalent to one cycle of the seminiferous epithelium in Wistar strain rats. Treatment of quinalphos for 13 days failed to produce any effect on the relative weights of the testes and accessory sex glands. However, significant inhibition of 3PHSD activity and increased cholesterol ]eve] in testis were observed. The rats treated for 26 days similarly showed a highly significant inhibition of the activity of both 30-HSD and 170-HSD. The relative weights of the testes and accessory sex glands were also significantly reduced. Histological examination of the testis revealed that quinalphos treatment produced detrimental changes in the seminiferous epithelium. Treatment with quinalphos for 13days produced no toxic effect with the exception of a significant increase in serum alkaline phosphatase. However, after 26days of treatment toxicity was significantly increased as reflected on serum transaminases, phosphatases and blood urea levels of rat. Present study indicated that quinalphos impairs testicular functions in rats. Der EinfluS von Quinalphos auf die testikulare SteroidbildungZusammenfassung: Anhand einer Behandlung mit 250 pg/kg Korpergewicht iiber 13 bzw. 26 rage wurde bei Wistarratten der EinfluP von Quinalphos, einem Organophosphat-Insektizid, auf die testikular steroidwirksamen Enzyme, 3-0-Hydroxysteroid-Dehydrogenase und 17-0-Hydroxysteroid-Dehydrogenase sowie auf Cholesterolgehalt und Histologie des Hodens untersucht.Das Zeitintervall von 13 Tagen entspricht ungefahr der Zyklusdauer des Keimepithels der Wistarratte. Die Behandlung mit Quinalphos iiber 13 Tage zeigte keinen EinfluP auf das relative Gewicht von Hoden und akzessorischen Geschlechtsdriisen. Dennoch wurden eine signifikante Hemmung der 3-0-HSD-Aktivitat und erhohte Cholesterolwerte im Hoden beobachtet. Die Ratten, welche iiber 26 Tage behandelt worden waren, zeigten gleichermalkn eine hochsignifikante Hemmung der 3-0-HSD und 17-0-HSD-Aktivitaten. Das relative Gewicht von Hoden und akzessorischen Geschlechtsdriisen war ebenfalls signifiiant verringert. Die histologische Untersuchung des Hodens deckte auf, dai3 Quinalphos schadliche Veriinderungen am Keimepithel verursacht. Die Behandlung mit Quinalphos iiber 13 Tage rief keinen toxischen Effekt hervor mit Ausnahme eines signifikanten Anstiegs der alkalischen Phosphatase im Serum. Die Behandlung iiber 16 Tage fiihrte zu einem signifikanten Anstieg der Toxizitiit, wie am Serumspiegel der Transaminasen und Phosphatasen sowie am Blutharnstoffspiegel festgestellt werden konnte. Die vorliegende Studie belegt, dai3 Quinalphos die testikularen Funktionen bei Ratten beeintdchtigt.

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Section: Introductionmentioning

confidence: 97%

Effect of Quinalphos on Testicular Steroidogenesis in Rats

et al. 2009

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“…This may be correlated with an HCH induced increase in Ca +2 -Mg +2 -ATPase activity of the testis. HCH has been reported to cause testicular hypertrophy and cellular damage when fed orally to mice (Nigam et al 1979). Intra-testicular injection of HCH to rats resulted in degeneration of seminiferous tubules and total or partial arrest of spermatogenesis (Dikshith and Datta 1972).…”

Section: Resultsmentioning

confidence: 99%

Age-Related Change in Rat Testicular ATPase Activities in Response to HCH Treatment

Roy

Chainy

1996

Bull. Environ. Contam. Toxicol.

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1 HCH (Y-hexachlorocyclohexane), an organochlorine insecticide is used widely for agricultural and hygienic purposes in most of the developing countries including India. Like other organochlorine, HCH can also enter into the body by various means such as through food chain, through inhalation and through dermal contact. It has been reported that HCH preferably accumulates in adipose tissues and in membrane lipid layer (Zhu et al. 1986; Lopez-Aparicio et al.1 988). Besides exhibiting neurological (Woolley et al. 1985;Tusell et al. 1987) and hepatological (Junqueira et al.1988) toxicities, HCH is also reported to produce adverse effect on male reproductive systems of rats. HCH-induced toxicity in male reproductive system includes degeneration of seminiferous tubules (Dikshith et al.1978 ; Srinivasan et a1.1988) and inhibition of stimulatory activity of adrenergic agonist upon CAMP accumulation in prostatic epithelial cells (Carrero et al. 1990). Mechanism of action of HCH on male reproductive system particularly in the testis is poorly understood. Mitochondrial fraction from rat testis has been shown to metaboiize ATP at a faster rate than that from liver or kidney (Hollinger 1971). Although testis from immature and mature rats catabolized ATP at a very high rate (Hollinger 1971), by means of Ca +2 and Mg +2 ATPase, there is a marked change in the enzyme activity during maturation (DelhumeauOngay et al.1973). It is understood that pesticide-induced testicular damage is more pronounced in immature rats in comparison to adult rats (Sjoberg et al.1985 Jinna et al.1989. Keeping this in view, an effort has been made in the present study to examine the effect of chronic exposure of HCH on testicular Ca +2 and Mg +2 -ATPase and Mg +2 ATPase in immature (30 days old) and adult (90 days old) rats since no information on this subject is yet available. MATERIALS AND METHODSMale wistar rats of 30 day old (weighing 60-80 g.) and 90 day old (weighing 300-350 g ) were purchased from National Institute of Nutrition, Hyderabad, India. They were acclimated to laboratory conditions for about 10 days before any experimentation. Rats were maintained with artificial illumination for 12 h followed by a dark period of 12 h. They were fed gram (Cicer arietinum) and freshly prepared diet containing flour and vitaminized powered milk. Tap water was supplied ad libitum.Rats of each age group were divided into three groups containing 5 animals per group. The second and third group were fed 10 mg and 20 mg HCH per kg. body weight respectively by oral intubation for 7, 15 and 30 days. The first group which served as control, was fed only olive oil in the similar manner. The technical grade HCH, obtained

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“…D'autres études ont mis en évidence, sur un plan anatomique, une diminution du poids des testicules et des glandes annexes (Srinavasan et al, 1988;Chowdhury et al, 1987;Pius et al, 1990). Sur un plan histo-logique, il a été décrit des altérations dégénératives des tubules séminifères (Dikshith et al, 1978), un ballonnement des cellules de Sertoli qui perdent leurs organites (Datta et Dikshith 1973;Dikshith et al, 1978;Nigam et al, 1979;Shivanandappa & Kris, 1983 ;Chowdhury et al, 1987;Dalsenter et al, 1996) et une dégénérescence des cellules de Leydig responsables de la synthèse de la testostérone (Chowdhury & Gautham, 1994). Enfin, le lindane entraîne sur un plan physiologique une diminution de la production des hormones stéroïdes et une réduction de la mobilité des spermatozoïdes (Chowdhury & Gautham, 1994.…”

Section: Lindane Administré Chez Le Mâle Adulteunclassified